Active Ganoderma lucidum polysaccharide peptide reference substance, and preparation method and application thereof
A technology of Ganoderma lucidum polysaccharide peptide and reference substance, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., and can solve the problems that cannot truly reflect the quality of Ganoderma lucidum products, cannot exclude the interference of excipients, and the content of polysaccharides in Ganoderma lucidum products is too high.
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Embodiment 1
[0064] 1. An active ganoderma polysaccharide peptide (GL-PPS) reference substance, which has the following characteristics:
[0065] (1) Properties: light brown to brown powder;
[0066] (2) Properties: soluble in water, insoluble in ethanol, acetone, ether, etc., easy to be affected by moisture;
[0067] (3) Purity: over 99%;
[0068] (4) Peak molecular weight (MP) 1.06~1.2×10 4 ;
[0069] (5) Chemical structure: composed of rhamnose (Rha), mannose (Man) and glucose (Glc), containing 17 kinds of amino acids, and the glycosidic bond is β-configuration;
[0070]
[0071] Its main chain is composed of 1,3- and 1,4-glucose alternately connected, and there are branches at the 6-position of part of the main chain glucose, and the non-reducing ends are all composed of glucose, and the main chain is also composed of 1, 6 or 1 , the composition of 3-linked β-D-Glcp, with branches at the 2 or 3 position of the main chain glucose of part 1.6-;
[0072] (6) The percent molar rati...
Embodiment 2
[0075] A preparation method of active ganoderma lucidum polysaccharide peptide (GL-PPS) reference substance, its steps are as follows:
[0076] (1) Select the fruiting body of Ganoderma lucidum, cut it into thin slices (2-20mm) or crush it, pass through a 40-60 mesh screen, add 10-30 times the volume of deionized water, soak it at 90°C for 2 hours, 90-100 Extract at ℃ for 1.5-2.0 hours, stir and stir intermittently, centrifuge at 3000-4000rpm, remove the precipitate and take the extract, add water 5-10 times the weight of the atomic entity to the residue after centrifugation, repeat the extraction twice, and combine the extracted supernatant;
[0077] (2) Concentrate the supernatant to 5-10 times of the original feeding amount, centrifuge to remove impurities, leave the clear liquid, and dialyze (36MD) for 48 hours, concentrate half of the dialysate, and centrifuge again (10000-12000rpm) to remove impurities , keep the supernatant;
[0078] (3) Slowly add three times the volu...
Embodiment 3
[0092] Example 3. The method for detecting the content of ganoderma lucidum polysaccharide peptide products using GL-PPS as a reference substance:
[0093] 1. Detection condition test
[0094] 1.1 Selection of wavelength: scan on a spectrophotometer with 0.25% GL-PPS (900-450nm), the maximum absorption peak is at 761.50nm, and the absorption value is 0.509. Therefore, the detection absorption wavelength is selected as 760nm.
[0095] 1.2 Preparation of test solution
[0096] 1.2.1 Preparation of Ganoderma lucidum test solution: Accurately weigh 5 g of crushed Ganoderma lucidum fruiting body and place it in a 100 mL Erlenmeyer flask, add 20 times the volume of distilled water, ultrasonicate at 60°C for 15 minutes, cool and filter to obtain the filtrate ①. Add 10 times the volume of distilled water to the filter residue, sonicate at 60°C for 15 minutes, cool and filter to obtain the filtrate ②. Combine the filtrates from ① and ②, concentrate by evaporation, centrifuge at 4000...
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