Preparation and detection method of mercury ion electrochemical biosensor

A biosensor and detection method technology, applied in the field of bioelectrochemistry, can solve the problems of high detection cost, time-consuming operation, complicated instruments, etc., and achieve the effects of good selectivity, convenient portability and simple operation.

Active Publication Date: 2020-03-24
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, a variety of equipment for detecting mercury ions has emerged as the times require, such as atomic absorption spectroscopy (AAS), atomic fluorescence spectroscopy (AFS), inductively coupled plasma mass spectrometry (ICP-MS), etc., but the detection costs of these methods are high, The instrument is complicated, the operation is time-consuming, and requires a special person to operate

Method used

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  • Preparation and detection method of mercury ion electrochemical biosensor
  • Preparation and detection method of mercury ion electrochemical biosensor
  • Preparation and detection method of mercury ion electrochemical biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: Step 1. Weigh 0.1g chitosan and stir it and dissolve it in 10ml of dilute hydrochloric acid aqueous solution; configure concentrated NaOH aqueous solution, slowly add it to the chitosan aqueous solution, white solids are precipitated, and the precipitated The white solid was centrifuged and washed several times with absolute ethanol and stored in absolute ethanol; respectively weighed 0.55g Tacetic acid, 0.57g EDC•HCl and 0.345g NHS, added 20ml of absolute ethanol to it, and stirred in an ice bath And react for 1h; add the absolute ethanol solution of chitosan to the activated T-acetic acid solution, first stir in ice bath for 30min, then react at room temperature for 24h; centrifuge and wash with absolute ethanol several times, and dry Obtain CsT compound, the reaction structural formula of chitosan and thymine is as figure 1 shown.

[0048] Step 2: Put the gold electrode on the polishing cloth of 1.0, 0.3, 0.05μm respectively with 1.0, 0.3, 0.05μm Al 2...

experiment example （1）

[0080] Experimental example (1): In the presence of 50 μmol / L Fc and Ru 3+ In the PBS (0.1M, pH=7.2) solution, the redox currents of bare Au, Cs-Au, Cat-Cs-Au and Cat-CsT-Au modified electrodes were measured by CVs method, and the results were as follows image 3 Shown (A: T-acetic acid concentration gradient UV-visible absorption spectrum; B: T-acetic acid standard curve and linear regression equation). After modifying Cs, since Cs is non-conductive and is an insulating film, it is observed from the figure that there is almost no peak on the Cs-Au modified electrode, indicating that Cs is modified to the surface of the Au electrode. Subsequently, Cat was modified on the surface of the Cs-Au electrode. Due to the electrochemical activity of Cat, Cat-CsT has redox activity, which can interact with Fc-Ru 3+ Electron transfer occurs between, and the figure shows a strong Fc oxidation peak and Ru 3+ Reduction peak, amplified Fc oxidation peak current and Ru 3+ The peak current ...

experiment example （2）

[0081] Experimental example (2): Weigh 1 mg of T-acetic acid and dissolve it in 5 ml of deionized water to prepare an aqueous solution with a concentration of 0.2 mg / ml, and then dilute the T-acetic acid aqueous solution by 1000 times, 800 times, 600 times, 400 times, 200 times, 100 times, 80 times, 60 times, 40 times, 20 times, the concentration is 0.0002g / L, 0.00025g / L, 0.00033g / L, 0.0005g / L, 0.001g / L, 0.002g / L, 0.0025g / L, 0.0033g / L, 0.005g / L, 0.01g / L, and measure its absorbance of ultraviolet and visible light, such as Pic 4-1 , along the direction of the arrow, the concentration of T-COOH is successively 54 μM, 27 μM, 18 μM, 14 μM, 11 μM, 5.4 μM, 2.7 μM, 1.8 μM, 1.4 μM, 1.1 μM; draw a standard curve, Pic 4-1 , and calculate the linear regression equation: y=0.04797x+4.93397, the correlation coefficient is 0.998. Pic 4-1 , the thymine grafting rate of 75%CsT is 30.9%, Figure 4-2 , 95% CsT thymine grafting rate was 47.6%.

[0082] Then respectively measure the UV-vis...

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Abstract

The invention provides a preparation and detection method of a mercury ion electrochemical biosensor. The electrochemical biosensor comprises a double-electron mediator, an electrode modifier and a functional substance, wherein the double-electron mediator is an Fc-Ru < 3 + > mediator, the electrode modifier is catechol and chitosan, and the functional substance is thymine. The electrochemical sensor can specifically recognize mercury ions, can be applied to mercury ion detection in water environment protection and wastewater treatment, and has the advantages of high sensitivity, good selectivity, low cost, simplicity in operation, convenience in carrying, short analysis time and the like.

Description

technical field [0001] The invention relates to a preparation and detection method of a mercury ion electrochemical biosensor, belonging to the field of bioelectrochemistry. Background technique [0002] It is well known that mercury ions, as one of metal carcinogenic ions, can generate free radicals and cause serious health problems. Mercury ions can accumulate in organisms and cause various diseases of the nervous, immune, reproductive and gastrointestinal systems. As a kind of heavy metal ion, mercury ion has become one of the most dangerous pollutants in water, which is extremely destructive to nature and seriously threatens human health. Therefore, rapid and accurate detection of mercury ions has become a key issue. In recent years, a variety of equipment for detecting mercury ions has emerged as the times require, such as atomic absorption spectroscopy (AAS), atomic fluorescence spectroscopy (AFS), inductively coupled plasma mass spectrometry (ICP-MS), etc., but the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/416
CPCG01N27/327G01N27/4163
Inventor 刘正春来庆腾姚晓霞陈智睿丁家峰梁波
Owner CENT SOUTH UNIV
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