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Foot-and-mouth disease O-type antigen epitope polypeptide and its preparation method and application

An antigen peptide, foot-and-mouth disease technology, applied in the field of medicine, can solve the problems affecting the use of new vaccines, immune effects, economic problems, and inability to effectively protect animals, etc.

Active Publication Date: 2021-07-06
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In terms of research on new vaccines for foot-and-mouth disease, there have been research reports on genetically engineered subunit vaccines, foot-and-mouth disease virus vector vaccines, and genetically engineered vaccines for foot-and-mouth disease.
In addition, these vaccines are often less effective against currently circulating strains that have mutated and cannot effectively protect animals

Method used

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  • Foot-and-mouth disease O-type antigen epitope polypeptide and its preparation method and application
  • Foot-and-mouth disease O-type antigen epitope polypeptide and its preparation method and application
  • Foot-and-mouth disease O-type antigen epitope polypeptide and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the solid-phase synthesis of polypeptide antigen of foot-and-mouth disease synthetic peptide vaccine

[0025] The present invention studies the variation of the main antigenic sites of the FMD by determining the sequence of the newly popular strains of the domestic FMD and combining the sequences of the FMD vaccine strains, and counts the variation frequency of the main mutated amino acid sites, and at the same time combines computer-aided FMD Analysis and prediction of antigenic sites, chemical synthesis of possible antigenic site peptides, that is, according to the statistical variation frequency of variable sites, different amino acids are used at these sites to obtain a multiplicity covering all current possible variable sites A candidate polypeptide antigen. Furthermore, these candidate polypeptide antigens were screened through a large number of animal experiments, and the polypeptide antigens capable of inducing an immune response in animals with a...

Embodiment 2

[0057] Embodiment 2, the preparation of foot-and-mouth disease O type synthetic peptide vaccine

[0058] 1.1 Preparation of antigen aqueous phase

[0059] Weigh the synthetic peptide antigens represented by polypeptide 1 or polypeptide 2 prepared according to Example 1, mix them in a molar ratio of 1:1, and then dilute the total concentration of the synthetic peptide antigens to 50 μg / ml with sterile water for injection. The resulting antigen solution was filtered through a filter with a pore size of 0.2 μm and sterilized.

[0060] 1.2 Preparation of oil phase adjuvant

[0061] Sterilize the oil phase adjuvant at 50V at 121°C for 30min, and set aside.

[0062] 1.3 Emulsion of synthetic peptide vaccine

[0063] Clean the IKA emulsification equipment with 2000ml of sterilized distilled water for 3 times. Under the condition of 25-27°C, according to the volume ratio of the polypeptide antigen water phase and the adjuvant 1:1, first add the adjuvant into the emulsification ta...

Embodiment 3

[0065] Embodiment 3, Efficacy test of foot-and-mouth disease type O synthetic peptide vaccine

[0066] 1. Materials and methods

[0067] 1.1 Materials

[0068] 1.1.1 Synthetic peptide vaccine and control vaccine

[0069] Prepare the polypeptide antigen such as polypeptide 1 (sequence 1) and polypeptide 2 (sequence 2) selected in embodiment 3 according to embodiment 1, then prepare three batches of foot-and-mouth disease containing polypeptide 1 and polypeptide 2 mixed components according to embodiment 2 Type synthetic peptide vaccines, the corresponding batch numbers are: O1801, O1802, O1803, and the corresponding batch number O1804 of the vaccine made of polypeptide 1 (sequence 1), and the corresponding batch number O1805 of the vaccine made of polypeptide 2 (sequence 2).

[0070] Control vaccine: porcine foot-and-mouth disease type O inactivated vaccine, batch number: 201907002

[0071] 1.1.2 Test animals

[0072] Select the same species, 4 months old, weighing about ...

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Abstract

The invention provides a foot-and-mouth disease O-type antigen epitope polypeptide, a preparation method and application thereof. The foot-and-mouth disease O-type synthetic peptide vaccine includes an antigenic polypeptide, which is the polypeptide shown in Sequence 2. The foot-and-mouth disease synthetic peptide vaccine provided by the invention has good immune efficacy, and the antigen of the vaccine is a chemically synthesized polypeptide, does not contain foot-and-mouth disease virus nucleic acid particles, and has high safety. Therefore, the vaccine of the invention can effectively deal with the current epidemic strains of foot-and-mouth disease, has no biological safety, is easy to synthesize on a large scale, and has good application prospects. The invention also provides the preparation method of the polypeptide and the peptide vaccine and their pharmaceutical application.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular, the invention relates to a foot-and-mouth disease synthetic peptide vaccine and a preparation method thereof. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, highly contagious, febrile infectious disease of cloven-hoofed animals, which is widely distributed in the world. Foot-and-mouth disease is highly contagious and spreads rapidly. After infecting pigs, pigs, sheep and other livestock, it will lead to the death of young animals and a sharp decline in the production capacity of adult animals. Therefore, it seriously endangers the development of animal husbandry and the production and supply of meat and animal products. At present, foot-and-mouth disease has greatly blocked and restricted the market circulation and international trade of animals and animal products, causing huge economic losses to animal husbandry production in endemic countries and region...

Claims

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Application Information

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IPC IPC(8): C07K14/09A61K39/135A61P31/14
CPCA61K39/12A61K2039/552A61P31/14C07K14/005C12N2770/32122C12N2770/32134
Inventor 肖进吴冬荀毕建敏郭丽清张蕾蔡薇向王震
Owner CHINA ANIMAL HUSBANDRY IND
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