Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Ultrasmall gold nanoparticles for mitochondrial targeting and rapid renal metabolism in tumor cells

A nanoparticle, tumor cell technology, applied in antitumor drugs, wave energy or particle radiation treatment materials, medical preparations with inactive ingredients, etc. To improve the degree of enrichment and residence time, facilitate metabolism, and achieve good biosafety effects

Active Publication Date: 2021-09-14
ZHEJIANG UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such nanomaterials lack tumor or organelle targeting functions, are not conducive to accumulation in tumor tissues and interact with specific organelles, and have poor radiosensitization effects.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ultrasmall gold nanoparticles for mitochondrial targeting and rapid renal metabolism in tumor cells
  • Ultrasmall gold nanoparticles for mitochondrial targeting and rapid renal metabolism in tumor cells
  • Ultrasmall gold nanoparticles for mitochondrial targeting and rapid renal metabolism in tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1) Add the peptide CCEKEKVFrFKFrFKVGFLGVEKEKEKEKEKEK to 10 mL of chloroauric acid (1 mM) solution, and stir vigorously at room temperature for 15 minutes to make the peptide concentration reach 2 mM. Sodium borohydride was added to bring the concentration to 10 mM. After stirring vigorously at room temperature for 0.5-1 hour, a polypeptide-stabilized ultra-small gold nanoparticle suspension is obtained. Its transmission electron microscope pictures are shown in figure 1 .

[0044] 2) Put 10mL of the gold nanoparticle suspension obtained in step 1) into a dialysis centrifuge tube (molecular weight cut-off 3000Da), centrifuge at 5000 rpm for 10 minutes, and the solution containing excess polypeptide ligand and sodium borohydride will be collected at the bottom of the centrifuge tube, The ultra-small gold nanoparticles obtained by concentration are retained on the upper part of the centrifuge tube.

[0045] 3) The gold nanoparticles (Au@Res Peptide, 100 μg / mL) obtained ...

Embodiment 2

[0049] Same as Example 1, the difference lies in step 1): Add polypeptide CCEKEKVFrFKFrFKVGFLGVEKEKEKEKEKEK to 10mL of chloroauric acid (1mM) solution, stir vigorously at room temperature for 15 minutes, so that the concentration of the polypeptide reaches 0.25, 0.5, 1.0, 2.0mM respectively. Sodium borohydride was added to bring the concentration to 10 mM. After stirring vigorously at room temperature for 0.5-1 hour, the peptide-stabilized ultra-small gold nanoparticle suspension prepared under different raw material feeding ratios is obtained. The results of the relationship between the size of gold nanoparticles and the proportion of raw materials can be found in figure 2 . As the ratio of polypeptide to chloroauric acid gradually increased, the particle size of the obtained nano-gold gradually decreased. It can be seen that to obtain gold nanoparticles with a particle size below 5.5nm, the molar ratio of polypeptide to chloroauric acid needs to be 1-2:1.

Embodiment 3

[0051] Same as Example 1, the difference lies in step 1): in 10mL of chloroauric acid (1mM) solution, add different polypeptides respectively:

[0052] (1) CCEKEKVFrFKFrFKVGFLGVEKEKEKEKEKEK;

[0053] (2) CEKEKVFrFKFrFKVGFLGVEKEKEKEKEKEK;

[0054] (3) CVFrFKFrFKVGFLGVEKEKEKEKEKEK;

[0055] (4) CEKVFrFKFrFKVGFLGVEKEKEKEKEKEK.

[0056] Vigorously stir at room temperature for 15 minutes to make the peptide concentration reach 2.0 mM. Sodium borohydride was added to bring the concentration to 10 mM. After stirring vigorously at room temperature for 0.5-1 hour, a stable ultra-small gold nanoparticle suspension of different polypeptides is obtained. The results of the relationship between the size of gold nanoparticles and peptides can be found in Figure 6 . It can be seen that all four kinds of polypeptides can obtain gold nanoparticles with a particle size below 5.5nm.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses an ultra-small gold nanoparticle with the characteristics of tumor cell mitochondria targeting and rapid kidney clearance, with a diameter of 2.5-5.5nm, which is prepared by modifying the surface of the gold nanoparticle with a cell-penetrating peptide, and the cell-penetrating peptide is composed of FrFKFrFK sequence, VGPLGV sequence and EKEKEKEKEKEK sequence. The ultra-small gold nanoparticle of the present invention has a very small size, is beneficial to metabolism through the kidney, and has good biological safety. The multifunctional responsive surface is stable under normal physiological conditions. After responding to the tumor microenvironment, the internal mitochondrial targeting molecules are exposed, which are efficiently endocytosed by liver tumor cells and targeted to mitochondria, thereby improving the enrichment and concentration of gold nanoparticles in tumors. Dwelling time: Gold nanoparticles contact with mitochondria, under radiation irradiation, can more effectively destroy the structure and function of mitochondria, and play a better effect of radiosensitization.

Description

[0001] (1) Technical field [0002] The invention relates to an ultra-small gold nanoparticle with the characteristics of tumor cell mitochondria targeting and rapid renal clearance, its preparation method and application. [0003] (2) Background technology [0004] Radiation therapy for tumors is a local treatment method that uses radiation to treat tumors. Radiation includes α, β, γ rays produced by radioactive isotopes and x-rays, electron beams, proton beams and other particle beams produced by various x-ray therapy machines or accelerators. The role and status of radiotherapy in tumor treatment has become increasingly prominent, and it has become one of the main means of treating malignant tumors. About 70% of cancer patients need radiation therapy in the course of cancer treatment, and about 40% of cancers can be cured by radiation therapy. However, due to the large dose required for radiotherapy and a certain course of treatment, the problem of side effects during the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/64A61P35/00
CPCA61K41/0057A61K47/64A61P35/00
Inventor 吴李鸣毛峥伟羊红玉冯靖祎
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com