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DNA marker closely linked to wheat 7DL chromosome scab-resistant gene and application thereof

A technology for wheat scab and scab, applied in the field of SSR markers, can solve problems such as stability differences, and achieve the effect of improving the efficiency of breeding work

Active Publication Date: 2020-03-13
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Studies at home and abroad have shown that scab resistance is controlled by quantitative trait locus (QTL) (immunity genes have not been found yet). More than 250 QTLs for scab resistance have been reported at home and abroad, and these QTLs have different degrees of disease resistance. , a single QTL can explain 2.1% to 50.0% of the degree of disease resistance, and usually a QTL that explains a relatively large degree of disease resistance (eg >20%) is called a major QTL, but most of the reported QTLs are minor QTLs
In addition, there are also differences in the stability of these QTLs. Some QTLs only express disease resistance in certain genetic backgrounds and certain environmental conditions, while other QTLs are stable in different genetic backgrounds and various environmental conditions. Stable expression of disease resistance

Method used

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  • DNA marker closely linked to wheat 7DL chromosome scab-resistant gene and application thereof
  • DNA marker closely linked to wheat 7DL chromosome scab-resistant gene and application thereof
  • DNA marker closely linked to wheat 7DL chromosome scab-resistant gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: Acquisition and mapping of the SSR marker cau7DL278 of wheat head blight resistance QTL

[0059] 1. Identification of scab resistance

[0060] 154 recombinant inbred lines of Luke×AQ were tested for resistance to scab in a wheat production field in Suiping County, Henan Province (the county is a severe scab-affected area), and were sown in early October, using a complete block design , the test plot consists of a single row of 1m long, with a row spacing of 25cm, and about 40 seeds of a recombinant inbred line are sown in each plot. In the early stage of wheat flowering in the next year, choose a sunny and windless afternoon, use spray inoculation, use a sprayer to evenly spray the suspension of Gibberella spores to the wheat canopy, then cover the inoculated plants with plastic film, and moisturize overnight; repeat the next day For inoculation, within 72 hours after inoculation, the wheat canopy was sprayed and moisturized every hour for three to ten minut...

Embodiment 2

[0077] Example 2: Application of SSR marker cau7DL278 in the identification of QTL QFhb.cau-7DL for wheat head blight resistance

[0078] Wheat to be tested: 84 wheat varieties (see Table 2) that are popularized in the collection and production of wheat scab endemic areas such as Huanghuai, according to the statistical data of the National Agricultural Technology Extension Center of the Ministry of Agriculture, the planting area of ​​these varieties is relatively large, And their pedigrees show relatively large genetic diversity, so they have better representation. The wheat cultivar Sumai 3, which is highly resistant to scab at home and abroad, was used as a disease-resistant control.

[0079] Table 2 Tested wheat varieties

[0080]

[0081]

[0082]

[0083] 1. Determination of genotypes of SSR marker cau7DL278 in different wheat varieties

[0084] (1) extracting the genomic DNA of the wheat plant to be tested;

[0085] (2) taking the genomic DNA of the plant to ...

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Abstract

The invention discloses a DNA marker closely linked to a wheat 7DL chromosome scab-resistant gene and an application thereof. The invention provides a molecular marker cau7DL278 located on a 7DL chromosome and linked to wheat scab resistance. The marker is a marker closely linked to scab resistance QTL QFhb. cau-7DL on the wheat 7DL chromosome, and is a codominant marker with high linkage degree (genetic distance is 0.1 cM). The molecular marker provided by the invention has the characteristics of rapidity, accuracy and high efficiency, and can be used to detect the scab resistance QTL QFhb. cau-7DL on the wheat 7DL chromosome and rapidly and accurately screen plants with the locus, thus improving breeding efficiency, laying a theoretical foundation for breeding wheat varieties with high scab resistance and providing molecular marker-assisted selection methods.

Description

technical field [0001] The invention relates to the fields of molecular biology, plant pathology and crop genetic breeding, in particular to an SSR marker closely linked with a new scab-resistant gene on the wheat 7DL chromosome and its application. Background technique [0002] Wheat (Triticum aestivum L.) is the main food crop in the world, providing staple food for nearly 40% of the global population. At present, the global wheat planting area is about 220-240 million hectares 2 , the total global production in 2018 was about 750 million tons (http: / / faostat.fao.org / faostat); my country is the world's largest wheat producer and consumer, and wheat is mainly planted with common winter wheat, and the sown area is around 24 million hm 2 The total production accounts for about 17% of the world's total wheat production. The safe production of wheat is very important, but it is vulnerable to a variety of diseases, among which wheat head blight is one of the important diseases...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156Y02A90/10
Inventor 王振任俊达彭友良赵文生权威张忠军
Owner CHINA AGRI UNIV
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