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Transcranial electrical stimulating assembly, device, system and application

An electrical stimulation and transcranial technology, applied in applications, electrodes, electrotherapy, etc., can solve the problems of lack of transcranial electrical stimulation devices, achieve the effects of reducing pulsation, realizing long-term living imaging, and preventing external environmental infection

Active Publication Date: 2020-02-14
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, experiments using two-photon microscopy to study the mechanism of cortical plasticity produced by transcranial electrical stimulation are all acute experiments. The existing technology can only conduct short-term electrical stimulation observations, and there is a lack of long-term two-photon imaging transcranial electrical stimulation devices.

Method used

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  • Transcranial electrical stimulating assembly, device, system and application
  • Transcranial electrical stimulating assembly, device, system and application
  • Transcranial electrical stimulating assembly, device, system and application

Examples

Experimental program
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Embodiment 1

[0041] This embodiment provides a transcranial electrical stimulation component. refer to figure 1 and figure 2 As shown, there are imaging holes and installation holes on the metal head ring. The round glass piece covers the animal craniotomy cortex. There are silver wires and conductive tape around the glass piece. The round glass piece is covered with Compaite medical glue and dental cement fixed on the animal skull.

Embodiment 2

[0043] This embodiment provides the method for installing and using the transcranial electrical stimulation device in Embodiment 1, which specifically includes the following steps:

[0044] (1) Surgical instruments and transcranial electrical stimulation devices are sterilized in an autoclave;

[0045] (2) 8-week-old mice were fed normally, anesthetized with isoflurane gas, and fixed on a stereotaxic apparatus. Remove the local scalp and fascia, rinse with low-concentration cephalosporin (cephalosporin and saline ratio 1:250), and determine the craniotomy area of ​​the cranial window, the diameter of which can be 3-4mm. After craniotomy, the virus vector AAV-Syn-GCaMP6f-WPRE was injected into the exposed cortex to transfect the cortical neurons, thereby expressing the GCamp6f fluorescent protein in the neurons, and then realizing the later two-photon calcium imaging to study the calcium activity of the neurons Variety. After viral vector injection, the round glass slide and ...

experiment example 1

[0051] This experimental example is an electrical stimulation and two-photon imaging experiment performed using the mouse model in Example 2. The expression effect of GCamp6f fluorescent protein in neurons after 3 weeks of mouse cortical injection image 3 As shown in Figure A, it can be seen from the figure that neurons normally express GCamp6f fluorescent protein. Set the electrical stimulation parameters as bidirectional pulses, with a frequency of 50 Hz, a current intensity of 0.3 mA, and a duration of 2 seconds. Experimental results refer to image 3 As shown in panel B in , the calcium signal of neurons evoked by electrical stimulation through the silver wire changed significantly.

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Abstract

The invention discloses a transcranial electrical stimulating assembly, device and system and an application, and relates to the technical field of experiment equipment. The transcranial electrical stimulating assembly, device and system are used for performing animal skull electrical stimulation. The skull electrical stimulating assembly comprises a headband, a glass sheet, a wire and a conducting adhesive tape, wherein an imaging hole is formed in the headband; the glass sheet is used for being in contact with the cortex of the animal; the wire is used for surrounding the periphery of the glass sheet and is in contact with the skull of the animal; and the conducting adhesive tape is adhered to and covers the wire and the skull. Through the adoption of the transcranial electrical stimulating assembly, device and system and the application disclosed by the invention, long-term transcranial electrical stimulation of animals can be realized; and the transcranial electrical stimulating assembly can be used for researching the changes of cellular morphology and functions in the long-term electricity stimulating process of the animals under a sober state, and the plasticity mechanism generated by electrical stimulating can be further researched.

Description

technical field [0001] The present invention relates to the technical field of experimental equipment, in particular to a transcranial electrical stimulation component, device, system and application. Background technique [0002] At present, experiments using two-photon microscopy to study the mechanism of cortical plasticity produced by transcranial electrical stimulation are all acute experiments. The existing technology can only conduct short-term electrical stimulation observations, and there is a lack of long-term two-photon imaging transcranial electrical stimulation devices. Transcranial electrical stimulation requires repeated daily stimulation to be effective, so long-term imaging is important to study the plasticity mechanism of electrical stimulation. [0003] In view of this, the present invention is proposed. Contents of the invention [0004] The object of the present invention is to provide a transcranial electrical stimulation component, device, system an...

Claims

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Application Information

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IPC IPC(8): A61B5/00A61N1/36A61N1/04
CPCA61B5/0071A61N1/36014A61N1/0456A61N1/0496A61B2503/40A61B2503/42
Inventor 马增光杜晓琅魏良鹏毕慧玲谌辉罗明艳刘淑静
Owner TIANJIN MEDICAL UNIV
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