Tumor sample pretreatment method

Abstract

The invention proposes a tumor sample pretreatment method. The method comprises: performing a first cleaning treatment on a fresh tumor sample in a washing buffer solution; removing the sample after the first cleaning treatment, so as to remove fat, blood, necrosis and interstitial mass fraction greater than 18-22%. tissue; and performing a second cleaning process on the removed tissue. This method effectively reduces the probability of microbial contamination after tumor sample culture, and improves the success rate of tumor sample culture. In the method, fat, blood, necrosis, and tissue with a mass fraction greater than 18-22% are selected. Strictly limited, on the one hand, it can remove some pollution, on the other hand, it makes the second cleaning more thorough and effective, and also greatly improves the tissue parts with more tumor cell content, improves the subsequent enzymatic hydrolysis efficiency and increases the subsequent tumor cell culture. Number of successful clones.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular, the invention relates to a pretreatment method of tumor samples. Background technique [0002] In the existing tumor organoid culture methods, the main focus is on sample enzymatic hydrolysis and medium formulation, and there are few detailed plans for sample pretreatment, but the sample pretreatment process is one of the main reasons for culture failure. [0003] For example, in the existing tumor organoid culture technology, the activity of the sample is likely to decrease during the transfer process, and the reduction in the number of viable cells will lead to culture failure; One of the main reasons for the failure; after the sample is obtained, the existing technology lacks the standard for selective elimination, and often uses tissue parts with less tumor cell content for culture, resulting in a small number of successfully cultured clones. [0004] The above reasons are the mai...

AI Technical Summary

Problems solved by technology

[0002] In the existing tumor organoid culture methods, the main focus is on sample enzymatic hydrolysis and medium formulation, etc., and there are few detailed plans for sample pretreatment, but the sample pretreatment process is one of the main reasons for culture failure

[0003] For example, in the existing tumor organoid culture technology, the activity of the sample is likely to decrease during the transfer process, and the reduction in the number of living cells will lead to culture failure; One of the main reasons for the failure; after the sample is obtained, the existing technology lacks the standard for selective elimination, and often uses tissue parts with less tumor cell content for culture, resulting in a small number of successfully cultured clones

[0004] The above reasons are the main reasons for failure in the pretreatment process of tumor organoid culture, and this will greatly increase the time and price cost of tumor organoid culture

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