Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Co-crosslinking immobilization method of porcine pancreatic lipase

A porcine pancreatic lipase, co-crosslinking technology, applied in immobilized enzymes, biochemical equipment and methods, immobilized on/in organic carriers, etc., can solve the problem of high reactivity, excessive crosslinking of enzymes, loss of enzyme activity and other problems, to achieve the effect of improving the catalytic activity and improving the microenvironment

Active Publication Date: 2020-02-07
NINGBO UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most commonly used cross-linking agent is water-soluble glutaraldehyde, which has high reactivity and is difficult to control the dosage. It is easy to cause excessive cross-linking of the enzyme and cause a great loss of enzyme activity. In addition, the traditional cross-linking method often It is necessary to precipitate and aggregate enzyme molecules before cross-linking, which will not only cause waste of enzymes, but also block mass transfer channels, and cannot fully exert the catalytic efficiency of enzymes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Co-crosslinking immobilization method of porcine pancreatic lipase
  • Co-crosslinking immobilization method of porcine pancreatic lipase
  • Co-crosslinking immobilization method of porcine pancreatic lipase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] Enzyme immobilization

[0016] 1) Bisphenol A epoxy resin (brand name is E-44, epoxy value is 0.44, number average molecular weight is 454), three kinds of components of methanol and diethylenetriamine are mixed according to the mass ratio of 2: 2: 1, Stir and react in the range of 25-35°C for 4-5 hours, pour the mixture into water, wash the precipitate repeatedly with water to remove methanol and a small amount of amine, then put it in a vacuum oven and dry it at room temperature to obtain an aminated epoxy resin;

[0017] 2) Add epoxy resin amides and β-cyclodextrin into water at a molar ratio of 1:2.1 to 1:2.3, heat and stir until all epoxy resin amides are converted into molecular complexes and dissolved in water, and keep the The total mass concentration of the aqueous solution is in the range of 5 to 6wt.%.

[0018] 3) Dissolving porcine pancreatic lipase in a phosphate buffer solution with pH=7.0, keeping the enzyme concentration in the range of 1.0-7.0 mg / mL; ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a co-crosslinking immobilization method of porcine pancreatic lipase. Oil-soluble butanediol diacrylate is used as a cross-linking agent, reactants in an aqueous phase are amino-containing porcine pancreatic lipase and a supermolecular complex formed by aminated epoxy resin and beta-cyclodextrin, through a Michael addition reaction of dual bonds and amino, a co-crosslinking polymerization reaction is generated at a low temperature, and immobilized porcine pancreatic lipase of different loading capacities can be prepared. The cross-linking degree is controlled, so thatthe dispersibility is improved, and internal mass transfer microenvironment can be improved. The immobilized enzyme has high catalytic activity, when the loading capacity is 42mg of enzymes for 1g ofcarriers, the activity achieves a highest value.

Description

technical field [0001] The invention relates to the technical field of immobilized enzyme biocatalysis, in particular to a method for co-crosslinking and immobilizing porcine pancreatic lipase. The novel immobilized enzyme can be used for hydrolysis of fats and also for dynamic resolution of chiral compounds. Background technique [0002] Porcine pancreatic lipase (EC 3.1.1.3), also known as triacylglycerol hydrolase, is an important acyl hydrolase that can catalyze the hydrolysis of triglycerides to produce diglycerides, monoglycerides, glycerol and fatty acids. Its molecular weight is between 45,000 and 50,000 Da, and it is an acidic protein (the isoelectric point is 5.0). Structurally, porcine pancreatic lipase is ellipsoidal, with a three-dimensional size of 4.6nm×2.6nm×1.1nm. It contains a large number of hydrophobic amino acids, which can form hydrophobic regions, and its polarity index is 47. In terms of composition, except for isoleucine which is different from othe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N11/096C12N11/10
CPCC12N11/08C12N11/10C12N9/20C12Y301/01003Y02P20/50
Inventor 吴嘉沁张瑞丰李艳肖通虎龙能兵
Owner NINGBO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com