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Preparation method and application of molecularly imprinted fluorescence sensor for detecting two types of viruses simultaneously and visually

A fluorescent sensor and molecular imprinting technology, applied in the field of analytical chemical detection, can solve problems such as simultaneous detection of similar viruses and difficulty in imprinting, and achieve the effect of visual detection

Active Publication Date: 2020-01-31
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the larger size of the virus, the weaker the structure formed by its self-assembly, the more difficult imprinting is; at the same time, viral molecular imprinting usually relies on the specific recognition of the shell structure on the surface of the virus, because the structural units of similar viral shells are also similar[ Cumbo, A., Lorber, B., Corvini, P.F.X., Meier, W., Shahgaldian, P. Nat. Commun. 2013, 4, 1925– 1930.] Therefore, capturing and identifying multiple different viruses simultaneously is currently It is still a very big challenge, and it is difficult to realize the simultaneous detection of multiple similar viruses

Method used

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  • Preparation method and application of molecularly imprinted fluorescence sensor for detecting two types of viruses simultaneously and visually
  • Preparation method and application of molecularly imprinted fluorescence sensor for detecting two types of viruses simultaneously and visually
  • Preparation method and application of molecularly imprinted fluorescence sensor for detecting two types of viruses simultaneously and visually

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: A method for preparing a molecularly imprinted fluorescent sensor for simultaneous visual detection of two viruses

[0032] (1) Preparation of CdTe QDs: Mix 38.3mg tellurium powder with 40mg NaBH 4 Add it to a mixed solution of absolute ethanol (1.0 mL) and ultrapure water (1.0 mL), and react under nitrogen protection to obtain the NaHTe intermediate. 92.4mg Cd(NO 3 ) 2 2.5H 2 O and 63 μL of mercaptopropionic acid (MPA) were dissolved in 75 mL of ultrapure water, and the resulting solution was adjusted to a pH of 9.0–10.0 with NaOH (1.0 M), and dissolved oxygen was removed by nitrogen blowing. Subsequently, 1 mL of NaHTe solution was added to the above solution, refluxed at 100 °C for 1.5 h to obtain G-QDs, and refluxed for 30 h to obtain R-QDs.

[0033] (2)G-CdTe@SiO 2 and R-CdTe@SiO 2 Preparation: Add 5 mL of G-QDs (R-QDs) into 40 mL of ethanol, then add 20 μL of 3-aminopropyltriethoxysilane (APTES) and stir for 12 h, then, dropwise add 300 μL of tetrae...

Embodiment 2

[0037] Example 2: Characterization of the performance, morphology and structure of the G-MIPs / R-MIPs fluorescent sensor and intermediate products.

[0038] The structure and morphology of all the prepared materials were characterized by Fourier transform infrared spectrometer, potentiometer and scanning electron microscope. image 3 (A) is G-CdTe(a), G-CdTe@SiO 2 (b), G-CdTe@SiO 2 C=C(c), G-MIP(d)G-NIP; (B) is R-CdTe(a), R-CdTe@SiO 2 (b), R-CdTe@SiO 2 C=C(c), R-MIP(d) IR spectra of R-NIP particles. The absorption peaks of G-QDs and R-QDs appear at 1537-1555cm, respectively -1 , 1390-1398cm -1 Office; 1037-1043cm -1 is the stretching vibration peak of Si-O-Si, 786 cm -1 is the bending vibration peak of Si-O-Si, so it proves that the silica ground is coated on the quantum dot; the 1720-1728cm that appears after the template is eluted -1 The peaks are attributed to C=O stretching vibrations in zinc acrylate; NIPs show no significant difference compared to MIPs, indicati...

Embodiment 3

[0041] Example 3: Application of the G-MIPs / R-MIPs fluorescence sensor.

[0042] The experimental conditions of this example are: the mass ratio of G-MIPs to R-MIPs is 1:1.5, the dosage of G-MIPs and R-MIPs is 10 mg / mL, the pH is 7.5, the adsorption time is 20 min, and the temperature is 25°C. The specific implementation method is: take specific concentrations of HAV and HBV and add them to a 10 mg / mL mixed solution of G-MIPs and R-MIPs, adjust the pH of the entire system to 7.5, and measure the fluorescence intensity after shaking and adsorbing at 25°C for 20 minutes .

[0043] (1) Simultaneous detection and analysis of different concentrations of HAV and HBV by G-MIPs / R-MIPs fluorescent sensor

[0044] According to the above experimental steps, the HAV and HBV mixed solutions of different concentrations are detected and analyzed with the G-MIPs / R-MIPs fluorescent sensor of the present invention, the results are as follows Figure 6 As shown, the prepared sensor has an anal...

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Abstract

The invention provides a preparation method and application of a molecularly imprinted fluorescence sensor for detecting two types of viruses simultaneously and visually. According to the preparationmethod, two types of quantum dots are used as fluorescence signal sources, functional monomers and hydrophilic monomers are added simultaneously, HAV and HBV are used as templates to be imprinted on different carriers respectively, obtained imprinted polymers are mixed after the templates are eluted, and the fluorescence sensor capable of detecting the two types of viruses simultaneously is obtained. Through the sensor, the advantages of the hydrophilic monomers N-isopropyl acrylamide (NIPAAm) and the advantages of the functional monomers zinc acrylate are combined simultaneously, and high-specificity recognition of the two types of viruses HAV and HBV is realized. Since the quantum dots have different fluorescence quenching degrees after being combined with target objects with different concentrations, visual detection is realized. The provided strategy lays a good foundation for simultaneously detecting multiple similar viruses through virus molecule imprinting and meanwhile has a potential ability in clinical treatment and diagnosis of viral diseases.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry detection, and in particular relates to a preparation method and application of a molecularly imprinted fluorescent sensor for visually detecting two viruses at the same time. Background technique [0002] Molecular imprinting technology has attracted more and more attention and application in the field of virus detection because of its advantages of easy preparation, high stability, sensitivity and selectivity. However, due to the larger size of the virus, the weaker the structure formed by its self-assembly, the more difficult imprinting is; at the same time, viral molecular imprinting usually relies on the specific recognition of the shell structure on the surface of the virus, because the structural units of similar viral shells are also similar[ Cumbo, A., Lorber, B., Corvini, P.F.X., Meier, W., Shahgaldian, P. Nat. Commun. 2013, 4, 1925– 1930.] Therefore, capturing and identifyi...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/569G01N33/576G01N33/58
CPCG01N21/6428G01N33/56983G01N33/576G01N33/582G01N2021/6432Y02A50/30
Inventor 蔡昌群陈思宇罗谅晖陈小明
Owner XIANGTAN UNIV
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