Acarus-killing traditional Chinese medicine composition, as well as preparation method and application thereof
The technology of a composition and a traditional Chinese medicine component is applied in the field of acaricide-removing traditional Chinese medicine composition and its preparation field, which can solve the problems of high toxicity of pesticides and strong smell of sulfur, achieve high-efficiency extraction, energy-saving optimization, and prevent the mating of mites. effects of reproduction
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Embodiment 1-3
[0041] Embodiment 1-3 a kind of anti-mite Chinese medicine composition
[0042] A traditional Chinese medicine composition for removing mites, the composition of which is shown in Table 1.
[0043] Table 1 embodiment 1-3 Chinese medicine composition (by weight)
[0044]
[0045]
[0046] The preparation method of the above-mentioned mite-removing traditional Chinese medicine composition: remove impurities, wash, and dry the raw materials of Chinese medicine, crush them, pass through a 40-mesh sieve, and mix them evenly to obtain a compound Chinese medicine mixture; add the compound Chinese medicine mixture to a volume 12 times the weight of the Chinese medicinal The fraction is 50% ethanol-water solution, placed in a conventional extraction tank, mixed and soaked for 3 hours, then the mixed solution of the Chinese medicine compound mixture and the solvent is passed through a continuous normal temperature micro-jet wall-broken extractor (referring to the public document C...
experiment example 1
[0055] Experimental example 1 Evaluation of mite removal effect
[0056] 1. Test object
[0057] The Chinese medicine composition extract prepared in Examples 1-3 and Comparative Examples 1-21 is diluted and dissolved with normal saline to form an aqueous solution with a mass fraction of 1.0%, as the test sample solution, and a portion of normal saline is prepared as the blank control sample solution at the same time .
[0058] 2. Test method
[0059] Each test sample solution and blank control sample solution are tested according to the following steps:
[0060] ①Prepare 3 covered glass cylinders with a bottom side length of 400mmx400mm and a height of 100mm as petri dishes, and put a sponge with a thickness of 10mm and a side length of about 200mm in each petri dish.
[0061] ②Inject the test sample solution into each petri dish (the height of the solution just submerges the sponge); and put 0.05g mite feed into each petri dish;
[0062] ③Put 200 surviving mites into each ...
experiment example 2
[0071] Experimental example 2 antibacterial experiment
[0072] 1. Test object
[0073] The Chinese medicine composition extracts prepared in Examples 1-3 and Comparative Examples 1-21 were diluted and dissolved with physiological saline to prepare an aqueous solution with a mass fraction of 1.0%, which was used as a test sample solution.
[0074] 2. Test method
[0075] The tested bacteria were bacteria such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, Aspergillus niger and Propionibacterium acnes. Cultured under the following conditions respectively, the bacteria were cultured on lecithin Tween 80-nutrient agar medium at 30-37°C for 36-48 hours; Aspergillus niger was cultured on Bengal Red medium at 20-25°C for 120 hours; Potato dextrose agar medium, cultivated at 20-25°C for 24-48 hours. The bacterial concentration was diluted to 1.0×10 9 CFU / mL, the concentration of Propionibacterium acnes was diluted to 1.0×10 9 CFU / mL, the Aspergillus niger...
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