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Lactobacillus plantarum LN66 and application thereof to product reducing halitosis risk

A technology of Lactobacillus plantarum and Fusobacterium nucleatum, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of exacerbation of bad breath symptoms, poor treatment effect, side effects and normal oral flora

Active Publication Date: 2020-01-21
JIAXING INNOCUL PROBIOTICS CO LTD
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  • Application Information

AI Technical Summary

Problems solved by technology

After the odor-causing bacteria form a biofilm, their resistance to antibiotics is significantly improved and it is difficult to leave the mouth with washing and washing, which will aggravate the symptoms of bad breath
At present, the main problem of using antibiotics to treat halitosis is that the resistance to antibiotics is greatly improved after the formation of biofilm by odor-causing bacteria, and excessive use of antibiotics will also cause some side effects and imbalance of normal oral flora, etc. The recurrence rate is also relatively low after stopping treatment high
It is also difficult to completely remove the odor-causing bacteria biofilm by mechanical scrubbing
The treatment of halitosis with probiotics is also receiving more and more attention. At present, the main choice is to choose probiotics that can inhibit the odor-causing bacteria or can neutralize the VSCs produced by the odor-causing bacteria, but the treatment effect is not good.

Method used

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  • Lactobacillus plantarum LN66 and application thereof to product reducing halitosis risk
  • Lactobacillus plantarum LN66 and application thereof to product reducing halitosis risk
  • Lactobacillus plantarum LN66 and application thereof to product reducing halitosis risk

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Experimental program
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Effect test

Embodiment 1

[0027] 1. Glucose metabolism test

[0028] After the LN66 strain was cultured at 37°C for 24 hours on the MRS solid medium, a single colony on the plate was scraped, and the biochemical reaction was detected using the API 50CH biochemical identification kit. The results are shown in Table 1:

[0029] Table 1 Lactobacillus plantarum LN66 sugar metabolism profile test results

[0030]

[0031]

[0032] Note: "+" means positive, "-" means negative

[0033] The results showed that the LN66 bacterial strain belonged to the genus Lactobacillus and the species Lactobacillus plantarum.

[0034] 2. Enzyme metabolism test

[0035] The enzyme activity of LN66 strain was semi-quantitatively analyzed with API ZYM kit (Mérieux, France). According to the operation instructions of the kit, the bacterial cells grown in the MRS culture medium were collected, and the bacterial suspension was prepared with sterile water for the next step of enzyme activity analysis. After adding bacteri...

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Abstract

The invention provides a lactobacillus plantarum LN66 and an application thereof to a product reducing a halitosis risk and relates to the technical field of halitosis prevention and treatment. An LN66 strain is preserved with the number of CGMCC No. 17369. The LN66 strain is directly separated from a healthy human body, is a Gram staining positive bacillus, is incapable of generating spores and is capable of growing in both aerobiotic and anaerobic environments. The LN66 strain has good tolerance to lysozyme and adapts to the oral environment, not aims at killing fusobacterium nucleatum, butaims at reducing the occurrence and development risks of halitosis by reducing the expression level of genes relevant to the formation of a biological film in the fusobacterium nucleatum, reducing thesecretion of extracellular polymeric substances (EPSs) of the fusobacterium nucleatum and specifically inhibiting or reducing the formation of fusobacterium nucleatum biological films.

Description

technical field [0001] The invention belongs to the technical field of halitosis prevention and treatment, and in particular relates to Lactobacillus plantarum LN66 and its application in products for reducing the risk of halitosis. Background technique [0002] Halitosis is a common clinical symptom, and even affects people's social psychology when it is severe. The formation of bad breath is mainly caused by some oral odor-causing bacteria decomposing the residues in the oral cavity to form unpleasant volatile sulfides (VSCs). Fusobacterium nucleatum is one of the main oral odor-causing bacteria. In addition to forming VSCs, it also forms oral Odor-causing bacteria are indispensable bacteria for biofilms, and play a crucial role in bridging the formation of biofilms. After the odor-causing bacteria form a biofilm, their resistance to antibiotics is significantly improved and it is difficult to leave the mouth with washing and washing, which will aggravate the symptoms of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/02C12R1/25
CPCA61K35/747A61P1/02C12N1/20C12N1/205C12R2001/25
Inventor 不公告发明人
Owner JIAXING INNOCUL PROBIOTICS CO LTD
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