Rapid identification method for soybean varieties with different phosphorus efficiency genotypes
An identification method and genotype technology, applied in the field of rapid identification of soybean varieties with different phosphorus efficiency genotypes, can solve the problems of long test period, heavy workload, slow test progress, etc., so as to reduce the overall screening workload and work cycle. Effect
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[0028] 1 cultivated soybean
[0029] Adopt sand cultivation method to cultivate soybeans, and the cultivation plate used is made of PVC pipe, with an internal diameter of 16 cm and a height of 25 cm. Three small holes with a diameter of 0.2 cm are drilled at the bottom of the basin. The quartz sand after every basin is filled to the basin mouth 3-4cm place (the weight of quartz sand is about 5kg). Two treatments of normal phosphorus and low phosphorus were set up for each variety. Potassium dihydrogen phosphate (KH 2 PO 4 ), repeated three times, and randomized blocks.
[0030] Sow five seeds per pot, pour 500mL distilled water at 8:00 every day, and pour 1000mL distilled water at 4:00 p.m. Pour 500mL of semi-nutrient solution every hour, and pour 1000mL of distilled water at 4 o'clock in the evening. After one week, change the semi-nutrient solution to full nutrient solution until the soybeans mature.
[0031] The phosphorus concentration in the nutrient solution used fo...
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