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protein-fes 2 Bioconjugated nanofibers and their preparation and use

A technology of nanofiber, -fes2, applied in the field of nano

Active Publication Date: 2022-05-20
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the construction of non-templated protein nanostructures has rarely been reported

Method used

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  • protein-fes <sub>2</sub> Bioconjugated nanofibers and their preparation and use
  • protein-fes <sub>2</sub> Bioconjugated nanofibers and their preparation and use
  • protein-fes <sub>2</sub> Bioconjugated nanofibers and their preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 (FeS 2 @BDA@Au optimal preparation scheme): In 9mL aqueous solution, add 2mmol bovine serum albumin and 2mmol dopamine (molar ratio of bovine serum albumin to dopamine 1:1), and stir magnetically for the first time at room temperature for 30min to make the solution evenly mixed. At room temperature, add ferrous ammonium sulfate solution to the above protein solution (volume of ferrous ammonium sulfate solution is 1mL, molar ratio of bovine serum albumin to ferrous ammonium sulfate is 2:3), and after magnetic stirring for the second time for 30min, adjust After the pH value of the mixed solution was 9, the sodium sulfide solution was added to the mixed solution of the protein and ammonium ferrous sulfate (the volume of the sodium sulfide solution was 1 mL, and the mol ratio of sodium sulfide and ammonium ferrous sulfate was 4:1), and the third magnetic After stirring for 30min, transfer to a water bath at 25°C and stir magnetically for 6h to obtain FeS 2 @B...

Embodiment 2

[0023]Example 2: In 9mL aqueous solution, add 2mmol bovine serum albumin and 1mmol dopamine (the molar ratio of bovine serum albumin to dopamine is 2:1), and stir magnetically for the first time at room temperature for 30min to make the solution evenly mixed. At room temperature, add ferrous ammonium sulfate solution to the protein solution (the volume of ferrous ammonium sulfate solution is 1 mL, the molar ratio of bovine serum albumin to ferrous ammonium sulfate is 2:3), and after magnetic stirring for the second time for 30 min, After the pH value of the mixed solution was adjusted to 9, the sodium sulfide solution was added to the mixed solution of the protein and ammonium ferrous sulfate (the volume of the sodium sulfide solution was 1 mL, and the mol ratio of sodium sulfide to ammonium ferrous sulfate was 4:1), the third time After magnetic stirring for 30 minutes, transfer to a water bath at 25°C and magnetically stir for 6 hours to obtain FeS 2 @BDA nanofibers. Will F...

Embodiment 3

[0024] Example 3: In 9 mL of aqueous solution, 2 mmol of bovine serum albumin and 2 mmol of dopamine were added (the molar ratio of bovine serum albumin to dopamine was 1:1), and the solution was mixed uniformly by magnetic stirring for the first time at room temperature for 30 min. At room temperature, add ferrous ammonium sulfate solution to the protein solution (the volume of ferrous ammonium sulfate solution is 1 mL, the molar ratio of bovine serum albumin to ferrous ammonium sulfate is 2:1), and after magnetic stirring for the second time for 30 min, After the pH value of the mixed solution was adjusted to 9, the sodium sulfide solution was added to the mixed solution of the protein and ammonium ferrous sulfate (the volume of the sodium sulfide solution was 1 mL, and the mol ratio of sodium sulfide to ammonium ferrous sulfate was 4:1), the third time After magnetic stirring for 30 minutes, transfer to a water bath at 25°C and magnetically stir for 6 hours to obtain FeS 2 ...

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Abstract

The invention belongs to the field of nanotechnology and relates to a gold nano-modified protein-FeS 2 Preparation of bioconjugated nanofibers and their use in bacterial inhibition. Specifically, dopamine was modified to bovine serum albumin by covalent coupling and used as a template to regulate protein assembly through the coordination of ferrous ions and the cross-linking effect of sulfide ions to prepare metal sulfide-induced biocrosslinking. protein nanofibers. The gold nanoparticles are modified on the surface of the protein nanofibers through the affinity between the sulfhydryl groups of the protein and the gold ions. The diameter of the nanofiber is about 2 μm. The invention prepares gold nano-modified protein-FeS2 biocoupled nanofibers, wherein the molar ratio of bovine serum albumin: ferrous ion: gold ion is 2:3:10, has good photothermal performance and can be used for the inhibition of bacteria.

Description

technical field [0001] The invention belongs to the field of nanotechnology and relates to a gold nano-modified protein-FeS 2 Preparation of bioconjugated nanofibers and their use in bacterial inhibition. Background technique [0002] Protein nanostructures are ubiquitous in nature, and proteins maintain cell growth by providing functional structural framework and molecular recognition. Protein nanostructures have potential applications in catalysis, biotechnology, and biomedicine. Finding appropriate methods to construct functionalized protein nanostructures has received increasing attention. Amino acid side chains of proteins can interact with organic dyes through hydrophobic interactions to form photothermal nanoparticles for biomedicine [Lu W,L; Lan Y.Q.; Xiao,K.J.; Xu,Q.M.; Qu,L.L.; Chen,Q.Y. ; Huang, T.; Gao, J. Zhao Y. BODIPY-MnNanoassemblies for accurate MRI and phototherapy of hypoxia cancer. J. Mater. Chem. B 2017, 5, 1275-1283; Mu W.Y.; .; Chen, Q.Y. A near-in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01F4/00D01F1/10D06M11/83D06M101/10
CPCD01F4/00D01F1/10D06M11/83D06M2101/10
Inventor 陈秋云穆威宇
Owner JIANGSU UNIV
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