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A kind of method for continuous enzymatic acylation of anthocyanin

A technology for acylation of anthocyanins and anthocyanins, which is applied in the field of acylation of anthocyanins, can solve problems such as poor selectivity, many steps, and severe reaction conditions, and achieve improved enzyme utilization, mild process conditions, and simple operation Effect

Active Publication Date: 2021-06-29
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many deficiencies and defects in the traditional chemical acylation method, such as poor reaction regioselectivity, easy to produce by-products, protective measures must be taken, many steps, long time-consuming, severe reaction conditions, and many solvents introduced, making separation and purification difficult
However, enzymatic acylation has the characteristics of mild reaction conditions, strong selectivity and high product quality, but the utilization rate of the enzyme in the enzymatic acylation reaction and the yield of the enzymatic acylation reaction are still low and need to be further improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This embodiment includes the following steps:

[0035] (1) Preparation of the immobilized enzyme column: load the macroporous resin immobilized enzyme into the column, the enzyme carrier is divinylbenzene resin, the enzyme is CALB, the column volume is 200mL, the diameter-to-height ratio is 1:4, and the volume fraction is 85 % ethanol washes the column 5BV at a flow rate of 0.5BV / h;

[0036] (2) Preparation of substrate: Dissolve 20g of anthocyanins (extracted from roselle, with an anthocyanin content of 79.3%) in 280g of ethanol with a volume mass fraction of 85%, and then add 110g of malic acid and 0.01g of Sodium ascorbate is fully stirred and dissolved at room temperature to obtain the substrate;

[0037] (3) Enzymatic acylation: put the substrate in step (2) on the immobilized enzyme column at a flow rate of 0.5 BV / h, keep the column temperature at 65±5°C, and collect the effluent.

[0038] (4) Low-temperature alcohol removal: Concentrate the effluent collected i...

Embodiment 2

[0042] (1) Preparation of the immobilized enzyme column: load the macroporous resin immobilized enzyme into the column, the enzyme carrier is styrene resin, the enzyme is RML, the column volume is 200mL, the diameter-to-height ratio is 1:9, and the volume mass fraction is 95 % ethanol washes the column 3BV with a flow rate of 1BV / h;

[0043] (2) Preparation of substrate: Dissolve 20g of anthocyanin (extracted from blueberry, anthocyanin content of 92.01%) in 180g of ethanol with a volume mass fraction of 90%, and then add 70g of ascorbic acid and 0.004g of coenzyme Q , fully stirred and dissolved at room temperature to obtain the substrate;

[0044] (3) Enzymatic acylation: put the substrate obtained in step (1) on the immobilized enzyme column at a flow rate of 1 BV / h, keep the column temperature at 35±5°C, and collect the effluent.

[0045] (4) Low-temperature alcohol removal: Concentrate the effluent collected in step (2) with a vacuum concentrator at a concentration tempera...

Embodiment 3

[0049] (1) Preparation of the immobilized enzyme column: the macroporous resin immobilized enzyme is loaded into the column, the enzyme carrier is acrylate resin, the enzyme is PSL, the column volume is 200mL, the diameter-to-height ratio is 1:6, and the volume mass fraction is 90 % ethanol washes the column 4BV with a flow rate of 1BV / h;

[0050] (2) Preparation of substrate: Dissolve 20g of anthocyanin (extracted from blueberry, anthocyanin content of 87.96%) in 140g of ethanol with a volume mass fraction of 95%, and then add 90g of lauric acid and 0.016g of β carrot The element was fully stirred and dissolved at room temperature to obtain the substrate;

[0051] (3) Enzymatic acylation: put the substrate obtained in step (2) on the immobilized enzyme column at a flow rate of 0.5BV / h, keep the column temperature at 55±5°C, and collect the effluent.

[0052] (4) Low-temperature alcohol removal: Concentrate the effluent collected in step (3) with a vacuum concentrator at a co...

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Abstract

A method for continuous enzymatic acylation of anthocyanins, comprising the following steps: (1) preparation of a substrate: dissolving anthocyanins and an acyl group donor together in an alcohol solvent containing antioxidants to obtain a substrate; ( 2) Enzymatic acylation: Put the substrate obtained in step (1) on the immobilized enzyme column, and collect the effluent; (3) Low-temperature alcohol removal: Concentrate the effluent collected in step (2), and recover alcohols solvent to obtain a concentrated solution; (4) resin adsorption: dilute the concentrated solution obtained in step (3) and put it on an adsorption resin column, desorb it with an alcohol solvent, and collect the desorbed solution; (5) Concentrate and dry: put The collected desorption solution is concentrated and dried to obtain the acylated anthocyanin product. The method of the invention adopts continuous enzymatic acylation of anthocyanin, and the whole process condition is mild, environment-friendly, and is relatively suitable for large-scale production.

Description

technical field [0001] The invention relates to a method for acylation of anthocyanin, in particular to a method for continuous enzymatic acylation of anthocyanin. Background technique [0002] Anthocyanin widely exists in the cell fluid of plant organs such as roots, stems, leaves, flowers and fruits, and is an important plant pigment and physiologically active functional component. Anthocyanins are safe and non-toxic, and have strong anti-oxidation and free radical scavenging effects; they can reduce high blood lipids, glycerides, and cholesterol levels; they have multiple functions such as anti-mutation, anti-tumor, anti-allergy, and protection of gastric mucosa. Cyanin has great application potential in the fields of food, cosmetics and medicine. However, due to the poor stability of anthocyanin structure, its wide application is limited. Improving the stability of anthocyanin is an urgent technical problem to be solved in the development and utilization of natural anth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06
CPCC12P17/06
Inventor 何安乐李伟黄华学
Owner HUNAN HUACHENG BIOTECH
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