Organelle-targeted aggregation-induced emission material and preparation method thereof

Abstract

The invention discloses an organelle-targeted aggregation-induced emission material and a preparation method thereof. The method comprises the following steps: 1) taking a triphenylamine borate and anaryl bromide compound as raw materials, fully and completely dissolving in a toluene solution, adding a potassium carbonate solution and ethanol solvent, taking tetrakis (triphenylphosphine) palladium as a reaction catalyst, carrying out nitrogen reaction, adding extraction, washing, removing of solvent, and carrying out column chromatography purification to obtain a target material; wherein whenthe aryl bromide compound in the step 1 is 2,5-dibromopyridine and 2, 5-dibromopyrazine, a product obtained in the step 1 and methyl iodide are taken as raw materials and are dissolved in toluene,heating reaction is carried out, toluene solvent is removed, then sodium hexafluorophosphate and acetonitrile are added to be fully dissolved, stirring is carried out for 12-24 hours at the room temperature, and a target fluorescent product can also be obtained after water washing, solvent removal under reduced pressure and recrystallization. The preparation and purification cost is low; the producthas excellent fluorescence emission performance, and can realize specific targeting imaging performance research of a plurality of organelles in cells.

Description

technical field [0001] The invention relates to the technical field of organic fluorescent materials and biological imaging thereof, in particular to a class of cell organelle-targeted aggregation-induced luminescent materials and a preparation method thereof. Background technique [0002] Compared with inorganic fluorescent materials, organic fluorescent materials usually have excellent luminescent properties and biocompatibility, and have shown good application prospects in biomedical fields such as cell imaging. At the same time, the good structural tunability of organic fluorescent materials can also provide the possibility to further meet their in-depth applications in the field of bioimaging. However, it is worth noting that for traditional organic fluorescent materials, although they can show good luminous efficiency and brightness in a single molecular state such as a solution, their luminous brightness usually falls short in an aggregated state such as a solid state...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of poor luminescent performance of traditional organic fluorescent materials in the aggregated state, and to achieve specific targeted imaging in cells, the purpose of the present invention is to provide a class of organelle-targeted aggregation-induced luminescent materials and a preparation method thereof; In the solution state, the constructed aggregation-induced luminescent materials show more excellent fluorescence emission properties after forming aggregates; at the same time, this type of material also has better biocompatibility, and can be better applied to lipid droplets and mitochondria, etc. The specific targeted imaging of organelles provides a reliable method and approach for further dynamic observation of cell states and understanding of interactions between intracellular organelles, etc.

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