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A kind of preparation method of hydroxyapatite/plga double-layer scaffold

A hydroxyapatite and double-layer technology is applied in the field of preparation of hydroxyapatite/PLGA double-layer scaffolds to achieve the effects of simple preparation process, suitable biodegradability and high mechanical strength

Active Publication Date: 2021-10-01
SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned technical problems in the prior art, the present invention provides a method for preparing a hydroxyapatite / PLGA double-layer stent, the preparation method of this hydroxyapatite / PLGA double-layer stent is close to the existing The preparation method of the hydroxyapatite scaffold in the technology is cumbersome, which is not conducive to the technical problem of loading bioactive drugs

Method used

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  • A kind of preparation method of hydroxyapatite/plga double-layer scaffold
  • A kind of preparation method of hydroxyapatite/plga double-layer scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Preparation of α-TCP powder with uniform particle size

[0017] Calcium hydrogen phosphate and calcium carbonate are weighed at a molar ratio of 2:1, mixed in a pure water medium by wet ball milling, the ball milling speed is 400rpm, and the ball milling time is 2-4h. After ball milling, place them in an oven at 80°C to dry overnight. After drying, the powder is calcined in a muffle furnace at 1250-1400°C for 2-4 hours, then taken out, and rapidly cooled in a blast environment. Then use zirconia ball milling, use ethanol as the ball milling medium, wet ball milling at 450 rpm for 4 hours, and dry in an oven at 80°C to obtain α-TCP (α-tricalcium phosphate) powder with uniform particle size.

Embodiment 2

[0019] Preparation concentration is the gelatin solution (50-100g / L) of 5-10% (w (g) / v (ml)), the above-mentioned gelatin solution and glycerin, mass percent concentration are 1wt% glutaraldehyde solution by volume ratio 100 : 10:1 for mixing, and at the same time, the above-mentioned α-TCP powder of Example 1 was added to the mixed solution at a solid-to-liquid ratio of 0.6-1g / mL to the gelatin solution to obtain a self-curing calcium phosphate bone cement 3D printing "ink". The mixed solution of gelatin, glycerin and glutaraldehyde in the above ratio is used as the solidification solution of α-TCP, so that the bone cement blending slurry has suitable injectability and viscosity. The "ink" has good injectability and can pass 0.3- The 0.5mm printing needle is used for printing, and its viscosity meets the requirements of vertical accumulation at room temperature, which can be applied to vertical deposition 3D printing technology.

Embodiment 3

[0020] Embodiment 3 Preparation of hydroxyapatite / PLGA double-layer scaffold

[0021] Put the self-curing calcium phosphate bone cement 3D printing "ink" in the above example 2 into the low-temperature printing nozzle, and the discharge needle is selected as 0.3-0.5mm; put the PLGA particles into the high-temperature printing nozzle, and the molecular weight of the PLGA particles is 100,000-40 10,000, LA / GA is 75 / 25-90 / 10, both ends of the PLGA molecular chain can be terminated by ester groups, hydroxyl groups or carboxyl groups, the discharge needle is 0.17-0.4mm, and the printing temperature is 180-200°C. Using the dual-nozzle printing mode of the 3D printer, the two materials were printed sequentially according to the model design to obtain a hydroxyapatite / PLGA double-layer scaffold.

[0022] A PLGA base with a diameter of 6 mm and a double-layer scaffold of hydroxyapatite with a diameter of 4 mm were printed and prepared by the above method. After printing, the material ...

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Abstract

The invention provides a method for preparing a hydroxyapatite / PLGA double-layer stent, comprising a step of preparing α-tricalcium phosphate powder; a step of preparing calcium phosphate bone cement 3D printing solidification solution; and a step of preparing hydroxyapatite / PLGA double-layer stent, put the calcium phosphate bone cement 3D printing solidification liquid into the low-temperature printing nozzle; put PLGA particles into the high-temperature printing nozzle, use the dual-nozzle printing mode of the 3D printer, and print two materials in sequence according to the model design, After printing, the material was left at room temperature to allow the calcium phosphate cement to solidify naturally, and finally a hydroxyapatite / PLGA bilayer scaffold was obtained. The entire 3D printing process of the present invention is carried out at normal temperature, and the hydroxyapatite scaffold is generated by one-step reaction without high-temperature calcination to sinter the hydroxyapatite and remove the binder, and the preparation process is more convenient.

Description

technical field [0001] The invention belongs to the field of tissue engineering and relates to a 3D printing technology, in particular to a method for preparing a hydroxyapatite / PLGA double-layer scaffold. Background technique [0002] 3D printing technology has been used in the preparation of bone repair materials in tissue engineering, and the research materials are also extensive. In vivo degradable materials include degradable polyester polymer scaffolds, collagen, sodium alginate cross-linked scaffolds, hydroxyapatite Stone and other inorganic calcium scaffolds, etc. Among them, in the preparation process of 3D printing technology, the hydroxyapatite scaffold is mainly prepared by printing after compounding with an adhesive, and then calcining at a high temperature. [0003] The preparation method of the traditional hydroxyapatite scaffold requires a calcination process, which is cumbersome and is not conducive to loading bioactive drugs; due to the high-temperature ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/12A61L27/18A61L27/22A61L27/50A61L27/58
CPCA61L27/12A61L27/18A61L27/222A61L27/50A61L27/58A61L2430/02C08L67/04
Inventor 吴宁杨迪诚朱君
Owner SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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