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Combination protein and application thereof

A combination protein and annexin technology, applied in the biological field, to achieve good immune protection effect and high immune protection effect

Active Publication Date: 2020-01-07
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is currently no commercially available vaccine for dogs (the ultimate host) to prevent E. granulosus infection in dogs

Method used

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  • Combination protein and application thereof
  • Combination protein and application thereof
  • Combination protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Animal immune protection test

[0037] 1. Test grouping

[0038] See Table 1.

[0039] Table 1 Grouping of experimental animals

[0040]

[0041]

[0042] 2. Preparation of mixed vaccine

[0043]The rEgANX / rEgTIM recombinant protein and saponin Quil A were diluted with sterile PBS to prepare an immunization mix containing 100 μg rEgANX, 100 μg rEgTIM and 1 mg Quil A per ml, respectively; an immunizing mix containing 200 μg rEgANX and 1 mg Quil A per ml; each ml Immunization mix containing 200 μg rEgTIM and 1 mg Quil A.

[0044] 3. Immunization program

[0045] Each dog in the experimental group was subcutaneously injected with 1 mL of the immunization mixture in the neck for two immunizations.

[0046] 4. Artificial infection

[0047] Two weeks of immunity, artificial infection was performed, and 70,000 protozoa were orally administered to each beagle dog in the experimental group and the control group.

[0048] 5. Blood collection

[0049] Befo...

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Abstract

The invention relates to the field of a biological technology, and discloses combination protein and an application thereof. The combination protein comprises combination protein comprising echinococcus granulosus annexin EgANX and triose phosphate isomerase EgTIM or fusion protein granule echinococcus granulosus annexin EgANX and triose phosphate isomerase EgTIM. The granule echinococcus granulosus annexin EgANX and the triose phosphate isomerase EgTIM are used as antigranule echinococcus granulosus annexin for immunization experiment, and results show that two kinds of protein have good immunoprotection effects on dogs, can produce a stable antibody, and have the potential for developing a granule echinococcus vaccine for the dogs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a combined protein and its application. Background technique [0002] Cystic hydatid disease, also known as cystic echinococcosis, is a worldwide zoonotic parasitic disease caused by the larvae of Echinococcus granulosus. The disease causes economic losses of about 3 billion yuan every year. The disease occurs in more than 20 provinces in my country, mainly in Qinghai, Tibet, Xinjiang and other places. It is estimated that there are about 350,000 to 1.3 million hydatid patients in China. Hydatid disease is a second-class animal disease in my country, and it is one of the 16 animal diseases that are prioritized and prevented in China. [0003] Echinococcus granulosus needs intermediate hosts (sheep, cattle and other livestock) and final hosts (dogs) to complete its life cycle. Blocking any part of the transmission route can effectively block the spread and infection of hydatid diseas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C12N9/90C07K19/00A61K39/00A61P33/10
CPCA61K39/0003A61P33/10C07K14/43554C07K2319/00C12N9/90C12Y503/01001
Inventor 杨光友华瑞其宋宏宇
Owner SICHUAN AGRI UNIV
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