Drug-loaded microspheres loaded with oncolytic virus and preparation method thereof
An oncolytic virus and drug-loaded microsphere technology, applied in the field of biomedicine, can solve the problems of insufficient therapeutic response, complicated administration of oncolytic virus, etc., achieve safe and convenient administration, improve intratumoral replication rate, and time release Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] Embodiment 1: A method for preparing drug-loaded microspheres loaded with anti-tumor monoclonal antibodies provided by the present invention comprises the following steps:
[0027] (1) dissolving the gelatin material methacrylylated gelatin and the photoinitiator LAP in a phosphate buffer to obtain a gelatin hydrogel solution, which is then used as the aqueous phase;
[0028] (2) configure an oily solution containing surfactant span 80, the mass percentage of surfactant span 80 in the oily solution is 2%, leave the oily solution for 6 hours to remove air bubbles, and use it as an oily phase for subsequent use;
[0029] (3) Connect the water phase prepared in step (1) and the oil phase prepared in step (2) to the micropump syringe respectively. The quality of the oil phase is three times that of the water phase. According to the particle size of the microspheres to be obtained Set the flow rate according to the size, and use blue light to irradiate the microsphere precur...
Embodiment 2
[0037] Embodiment 2: A method for preparing drug-loaded microspheres loaded with anti-tumor monoclonal antibodies provided by the present invention comprises the following steps:
[0038] (1) dissolving the gelatin material methacrylated gelatin and the photoinitiator LAP in a phosphate buffer to obtain a gelatin hydrogel solution, and the gelatin hydrogel solution is used as an aqueous phase for stand-by;
[0039] (2) configure an oily solution containing surfactant span 80, the mass percentage of surfactant span 80 in the oily solution is 2%, leave the oily solution for 3 hours to remove air bubbles, and use it as an oily phase for subsequent use;
[0040] (3) Connect the water phase prepared in step (1) and the oil phase prepared in step (2) to the micropump syringe respectively. The quality of the oil phase is at least three times that of the water phase, such as 5 times. The particle size of the microspheres is used to set the flow rate, and the blue light is used to irra...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com