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Drug-loaded microspheres loaded with oncolytic virus and preparation method thereof

An oncolytic virus and drug-loaded microsphere technology, applied in the field of biomedicine, can solve the problems of insufficient therapeutic response, complicated administration of oncolytic virus, etc., achieve safe and convenient administration, improve intratumoral replication rate, and time release Effect

Inactive Publication Date: 2020-01-03
兆科(广州)肿瘤药物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the defects in the prior art that the administration of oncolytic viruses such as Pexa-Vec is complicated and the treatment response is not sufficient by means of intravenous injection and intratumoral injection, the present invention provides a drug-carrying drug containing oncolytic virus The purpose of the microsphere and its preparation method is to position and target the tumor site through interventional administration, thereby enhancing the anti-tumor effect and realizing adjustable and controllable drug release.

Method used

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  • Drug-loaded microspheres loaded with oncolytic virus and preparation method thereof
  • Drug-loaded microspheres loaded with oncolytic virus and preparation method thereof
  • Drug-loaded microspheres loaded with oncolytic virus and preparation method thereof

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Embodiment 1

[0026] Embodiment 1: A method for preparing drug-loaded microspheres loaded with anti-tumor monoclonal antibodies provided by the present invention comprises the following steps:

[0027] (1) dissolving the gelatin material methacrylylated gelatin and the photoinitiator LAP in a phosphate buffer to obtain a gelatin hydrogel solution, which is then used as the aqueous phase;

[0028] (2) configure an oily solution containing surfactant span 80, the mass percentage of surfactant span 80 in the oily solution is 2%, leave the oily solution for 6 hours to remove air bubbles, and use it as an oily phase for subsequent use;

[0029] (3) Connect the water phase prepared in step (1) and the oil phase prepared in step (2) to the micropump syringe respectively. The quality of the oil phase is three times that of the water phase. According to the particle size of the microspheres to be obtained Set the flow rate according to the size, and use blue light to irradiate the microsphere precur...

Embodiment 2

[0037] Embodiment 2: A method for preparing drug-loaded microspheres loaded with anti-tumor monoclonal antibodies provided by the present invention comprises the following steps:

[0038] (1) dissolving the gelatin material methacrylated gelatin and the photoinitiator LAP in a phosphate buffer to obtain a gelatin hydrogel solution, and the gelatin hydrogel solution is used as an aqueous phase for stand-by;

[0039] (2) configure an oily solution containing surfactant span 80, the mass percentage of surfactant span 80 in the oily solution is 2%, leave the oily solution for 3 hours to remove air bubbles, and use it as an oily phase for subsequent use;

[0040] (3) Connect the water phase prepared in step (1) and the oil phase prepared in step (2) to the micropump syringe respectively. The quality of the oil phase is at least three times that of the water phase, such as 5 times. The particle size of the microspheres is used to set the flow rate, and the blue light is used to irra...

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Abstract

The invention discloses a preparation method of drug-loaded microspheres loaded with oncolytic virus. The preparation method of the drug-loaded microspheres loaded with the oncolytic virus comprises the following steps: (1) preparing an aqueous-phase gelatin-hydrogel solution; (2) preparing an oil-phase solution; (3) irradiating microsphere precursors made of a mixture of the aqueous phase and theoil phase at a collection end, so that the microsphere precursors cross-link to form no-load microspheres; (4) performing post-treatments, namely greasy-dirtf removing and freeze-drying, on the dulycross-linked no-load microspheres obtained in the step (3); and (5) mixing freeze-dried microspheres obtained in the step (4) with an oncolytic virus solution so as to obtain the drug-loaded microspheres loaded with the oncolytic virus. The preparation method of the drug-loaded microspheres loaded with the oncolytic virus has the following advantages: the microspheres are prepared by adopting bio-compatible materials, including gelatin and the like, and used in intervention of oncolytic virus administration; and thus, immunogenicity of intravenously administrated oncolytic virus can be reducedwith in-tumor replication rate of oncolytic virus improved and anti-tumor ability of a drug enhanced in local hypoxia micro-environment created by the drug-loaded microspheres.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a drug-loaded microsphere loaded with an oncolytic virus and a preparation method thereof. Background technique [0002] Oncolytic viruses (OV) refer to a class of native or recombinant viruses that can selectively kill tumor cells without damaging normal cells. Oncolytic virus therapy (Oncolytic virotherapy) is a new type of tumor-targeted therapy. It is an anti-tumor agent that uses tumor-selective and multi-killing oncolytic viruses as its main component. It can directly kill tumor cells to achieve oncolytic effects. , and release tumor-specific antigens in the process of tumor cell lysis, and activate the body's anti-tumor immune response. Currently, oncolytic poxviruses commonly used for genetic modification mainly include Lister strain, WR (Western Reverse) strain, Wyeth strain, Copenhagen strain and Tiantan strain. [0003] Pexa-Vec (pexastimogene dvacirepvec) virus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/42A61K35/768A61P35/00
CPCA61K9/5057A61K35/768A61P35/00
Inventor 胡海燕
Owner 兆科(广州)肿瘤药物有限公司
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