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Dictyophora rubrovalvata mother strain culture method

The technology of a kind of Dictyophora edulis and its cultivation method is applied in the field of cultivating the mother species of Dictyophora edulis, which can solve the problems of long cultivation time, weak strain vigor, and long growth cycle of the strain, and achieve low cost and easy to be harvested. The effect of absorption and short growth cycle

Pending Publication Date: 2020-01-03
贵州金蟾大山生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dictyophora edulis is a species with better quality among the edible ones. It has high medicinal value and nutritional value. It is the main species cultivated artificially in China. Excellent strains are the main prerequisite for obtaining high-quality Dictyophora edulis. However, the current Dictyophora spp. growth cycle is long, and there is a phenomenon that the activity of the bacteria is not strong; as disclosed in the document with the patent application number 201510841397.1, a method for cultivating the solid parent species of Dictyophora spp. uses sawdust, wheat Bran, corn flour, soybean flour, sugar, gypsum, and an appropriate amount of water are mixed and boiled for a period of time to make a culture medium. The mature bamboo eggs are used as the inoculation source, and the mother species of Dictyophora edulis is cultivated under specific conditions. The cultivation time is 35- 45 days, the cultivation time is relatively long; another example is a method for making the female species of Fanjingshan wild red-twisted bamboo fungus disclosed in the document with the patent application number 201510268986.5, which is made by mixing soil ginseng, rice, glucose, agar, and appropriate amount of water The culture medium uses the eggs of wild Dictyophora edulis in Fanjing Mountain as the inoculation source, and cultivates the mother species of Dictyophora edulis under specific conditions. The cultivation time is about 45 days, and the cultivation time is relatively long;

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for cultivating the mother species of Dictyophora edulis, specifically comprising the following steps:

[0025] (1) Preparation of medium:

[0026] a, take by weight 100g potatoes, 5g glucose, 5g maltose, 2g yeast extract powder, 1g peptone, 10g bamboo soil, 15g masson pine needles, 5g coarse sawdust, 10g fine wood chips, 18g agar powder;

[0027] b. Take bamboo plant soil, masson pine needles, and coarse miscellaneous sawdust, mix them evenly, add 1500ml of water to the mixture and boil on high heat, then decoct on low heat for 1 hour, filter with 4 layers of medical degreasing gauze, and obtain filtrate A;

[0028] c. Peel the potatoes, cut them into 2-3mm thick slices, add 1000ml of water to the slices and cook for 20 minutes until the potatoes are soft but not rotten, filter with 4 layers of medical absorbent gauze to obtain filtrate B;

[0029] d. Mix filtrate A and filtrate B evenly, add water to make up to 800ml, and obtain mixed solution A;

[0030] e...

Embodiment 2

[0043] A method for cultivating the Dictyophora edulis strain, specifically comprising the following steps:

[0044] (1) Preparation of medium:

[0045] a, take by weight 150g potatoes, 15g glucose, 20g maltose, 2.5g yeast extract powder, 1.5g peptone, 15g bamboo soil, 18g masson pine needles, 60g coarse miscellaneous wood chips, 15g fine wood chips, 19g agar powder;

[0046] b. Take bamboo soil, masson pine needles, and coarse sawdust, mix them evenly, add 1800ml of water to the mixture, boil it on high heat first, then decoct it on low heat for 1.5h, filter it with 5 layers of medical degreasing gauze, and obtain filtrate A;

[0047] c. Peel the potatoes, cut them into thin slices with a thickness of 2-3mm, add 1200ml of water to the thin slices and cook for 20-30min until the potatoes are soft but not rotten, then filter with 5 layers of medical absorbent gauze to obtain the filtrate B;

[0048] d. Mix filtrate A and filtrate B evenly, add water to make the volume to 1000m...

Embodiment 3

[0062] A method for cultivating the mother species of Dictyophora edulis, specifically comprising the following steps:

[0063] (1) Preparation of medium:

[0064] a, take by weight 200g potatoes, 20g glucose, 5g maltose, 2g yeast extract powder, 1g peptone, 20g bamboo soil, 20g masson pine needles, 80g coarse miscellaneous sawdust, 16g fine sawdust, 18g agar powder;

[0065] b. Take bamboo soil, masson pine needles, and coarse sawdust, mix them evenly, add 2000ml of water to the mixture, boil it on high heat first, then decoct it on low heat for 2 hours, and filter it with 4 layers of medical degreasing gauze to obtain filtrate A;

[0066] c. Peel the potatoes, cut them into 2-3mm thick slices, add 1500ml of water to the slices and cook for 30 minutes until the potatoes are soft but not rotten, then filter with 4 layers of medical absorbent gauze to obtain filtrate B;

[0067] d. Mix filtrate A and filtrate B evenly, add water to make the volume to 1000ml, and obtain mixed s...

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Abstract

The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a dictyophora rubrovalvata mother strain culture method which includes the steps: (1) culture medium preparation: taking potatoes, glucose, malt sugar, yeast extract powder, peptone, bamboo plain soil, masson pine needles, coarse miscellaneous wood chips, fine wood chips and agar powder as raw materials by a certain weight to prepare a culture medium; (2) inoculation: selecting suitable strains, and inoculating the suitable strains onto the culture medium by an inoculation needle; (3) culture: culturing the inoculated culture medium under specific conditions for 20-25 days to obtain dictyophora rubrovalvata mother strains. The dictyophora rubrovalvata mother strain culture method is simple to operate, low in cost, free from pollution and short in growth cycle, and prepared dictyophora rubrovalvata mother strains are high in growth force and good in quality.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, in particular to a method for cultivating the mother species of Dictyophora edulis. Background technique [0002] Dictyophora, also known as bamboo fungus and bamboo ginseng, are common and edible. There are 4 kinds: long skirt bamboo fungus, short skirt bamboo fungus, spiny bamboo fungus and red bamboo fungus. Flower fungi are rich in nutrition, rich in fragrance and delicious in taste. They are called "Fairy in Snow Skirt", "Flower of Mountain Treasure", "Flower of Fungus" and "Queen of Fungi". Dictyophora edulis is a species with better quality among the edible ones. It has high medicinal value and nutritional value. It is the main species cultivated artificially in China. Excellent strains are the main prerequisite for obtaining high-quality Dictyophora edulis. However, the current Dictyophora spp. growth cycle is long, and there is a phenomenon that the activity of the bac...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/40A01G18/20
Inventor 陈翠翠庄继文肖忠建方晔梅健余蓉巩玉辉韩勇
Owner 贵州金蟾大山生物科技有限责任公司
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