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Culture medium for improving cordycepin of cordyceps militaris and preparation method of culture medium

A culture medium and Cordyceps militaris technology, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve the problems of low Cordycepin militaris content, reduced use value, and increased input cost, so as to increase the content and promote proliferation Effects of differentiating and stimulating uptake

Inactive Publication Date: 2020-01-03
江门市山海堂虫草有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, the content of cordycepin in wild Cordyceps sinensis is extremely low, and it is generally extracted from Cordyceps militaris. Because the existing culture medium of Cordyceps militaris lacks substances that promote the synthesis of cordycepin, the content of cordycepin in Cordyceps militaris Low, which directly leads to the reduction of its use value and the increase of input cost

Method used

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  • Culture medium for improving cordycepin of cordyceps militaris and preparation method of culture medium

Examples

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Effect test

Embodiment 1

[0024] The invention provides a Cordyceps militaris culture medium for increasing the content of cordycepin in Cordyceps militaris, comprising a liquid fermentation medium and a fruiting body solid medium: according to the mass percentage, the composition of the liquid fermentation medium is: 15% potato, white sugar 1.5%, egg white 1%, beef extract 1%, adenine 0.1%, glycine 0.2%, distilled water 70%; the described fruit body solid medium consists of: rice 20%, crushed corn kernels 5%, white sugar 2% %, silkworm chrysalis powder 1%, adenine 0.5%, glutamine 1%, distilled water 55%.

[0025] The present invention also provides a kind of preparation method of Cordyceps militaris culture medium comprising the steps:

[0026] S1) raw material preparation: prepare the raw material of the liquid fermentation broth medium by mass percentage;

[0027] S2) Potato dissolving and subpackaging: peel the potatoes, squeeze the juice, mix and dissolve with the above materials, then subpackage...

Embodiment 2

[0033] A Cordyceps militaris culture medium for increasing the content of cordycepin in Cordyceps militaris, comprising a liquid fermentation medium and a fruiting body solid medium: according to the mass percentage, the composition of the liquid fermentation medium is: 17% of potatoes, 1.8% of white sugar, 1.5% of egg white, 1.5% of beef extract, 0.15% of adenine, 0.3% of glycine, 75% of distilled water; the solid medium for fruiting body consists of: 25% of rice, 8% of crushed corn kernels, 2.5% of white sugar, silkworm chrysalis powder 1.5%, adenine 0.75%, glutamine 1.5%, distilled water 60%.

[0034] The Cordyceps militaris medium described in Example 2 was prepared according to the preparation method of Example 1.

Embodiment 3

[0036] A Cordyceps militaris culture medium for increasing the content of cordycepin in Cordyceps militaris, comprising a liquid fermentation medium and a fruiting body solid medium: according to the mass percentage, the composition of the liquid fermentation medium is: 20% of potatoes, 2% of white sugar, 2% of egg white, 2% of beef extract, 0.2% of adenine, 0.4% of glycine, and 80% of distilled water; the solid culture medium for fruiting body consists of: 30% of rice, 10% of crushed corn kernels, 3% of white sugar, silkworm chrysalis Powder 2%, adenine 1%, glutamine 2%, distilled water 65%.

[0037] The Cordyceps militaris medium described in Example 3 was prepared according to the preparation method of Example 1.

[0038] The cordycepin content of embodiment 1-3 improves as described in table one

[0039]

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Abstract

The invention discloses a culture medium for improving cordycepin of cordyceps militaris. The culture medium comprises a liquid fermentation broth culture medium and a fruiting body solid culture medium, and is characterized in that the liquid fermentation broth culture medium comprises the following components in percentages by mass: 15%-20% of potatoes, 1.5%-2% of white granulated sugar, 1%-2% of egg white, 1%-2% of beef paste, 0.1%-0.2% of adenine, 0.2%-0.4% of glycine and 70%-80% of distilled water; and the fruiting body solid culture medium comprises the following components: 20%-30% of rice, 5%-10% of crushed corn kernels, 2%-3% of white granulated sugar, 1%-2% of silkworm chrysalis powder, 0.5%-1% of adenine, 1%-2% of glutamine and 55%-65% of distilled water. The invention also provides a preparation method of the culture medium for improving the cordycepin of the cordyceps militaris. By the liquid fermentation broth culture medium and the fruiting body solid culture medium andin combination of the preparation method, growth of cordyceps militaris cells and uptake to sugars can be stimulated effectively, and the content of the cordycepin is increased.

Description

technical field [0001] The invention relates to the technical field of Cordyceps militaris cultivation, in particular to a culture medium for increasing cordycepin militaris, especially a culture medium for improving cordycepin militaris and a preparation method thereof. Background technique [0002] Cordycepin, also known as cordycepin, cordycepin, and militaris, is the main active ingredient of Cordyceps sinensis and Cordyceps militaris; cordycepin has the functions of antibacterial, anti-tumor, anti-inflammatory, improving metabolism, and scavenging free radicals. Supplements are becoming an extremely active field in medicinal chemistry, anti-aging, beauty, and health products. [0003] The worldwide distribution of Cordyceps militaris has very few natural resources. It is a complex composed of two parts, the sub-seat (that is, the grass part) and the sclerotia (that is, the dead body part of the insect). In winter, the larvae hibernate in the soil, where the fungi para...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20
CPCA01G18/20
Inventor 邓翠珍
Owner 江门市山海堂虫草有限公司
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