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Gene and protein for quickening fruits to be mature, carrier, recombination gene engineering bacteria, and application of gene and protein

A technology of genetically engineered bacteria and proteins, applied in the field of agricultural biology, can solve problems such as undiscovered patent publications, achieve good application prospects, accelerate fruit ripening, and reduce sugar content

Active Publication Date: 2019-12-31
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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  • Gene and protein for quickening fruits to be mature, carrier, recombination gene engineering bacteria, and application of gene and protein
  • Gene and protein for quickening fruits to be mature, carrier, recombination gene engineering bacteria, and application of gene and protein

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Embodiment Construction

[0028] The embodiments of the present invention will be described in detail below. It should be noted that this embodiment is descriptive and not restrictive, and the protection scope of the present invention cannot be limited by this.

[0029] The raw materials used in the present invention are conventional commercial products unless otherwise specified; the methods used in the present invention, unless otherwise specified, are conventional methods in the field.

[0030] A gene for accelerating fruit ripening, the gene is: the coding region is the DNA molecule shown in SEQ ID NO.1;

[0031] Or: the coding region is a DNA molecule with 90% or more homology with the DNA molecule shown in SEQ ID NO.1.

[0032] The purpose of the present invention is to provide a gene for accelerating fruit ripening (FRA gene, Fruit ripening accelerator). Due to the particularity of the nucleotide sequence, any variant of the polynucleotide shown in SEQ ID NO.1, as long as it is compatible with the polynu...

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Abstract

The invention relates to a gene for quickening fruits to be mature. The gene is characterized in that a coding region is a DNA (deoxyribonucleic acid) molecule disclosed by SEQ ID NO.1, or the codingregion is the DNA molecule which is disclosed by SEQ ID NO.1 and has 90% homology and above. The invention mines a gene (FRA gene) which accelerates fruits to be mature in advance for the first time in addition to an ethylene biosynthesis approach, a carrier is constructed, a transgenosis method is used for applying the gene to the innovative research and development of the new germplasm of premature fruits, and an overexpress FRA gene can quicken the fruits to be mature.

Description

Technical field [0001] The invention belongs to the field of agricultural biotechnology, in particular to a gene (FRA gene), protein, carrier, recombinant genetic engineering bacteria and application thereof for accelerating fruit ripening. Background technique [0002] The phenomenon of fruit ripening is mainly controlled by a series of physiological and biochemical reactions triggered by ethylene. In the fruit ripening process, respiration is strengthened, starch is hydrolyzed into soluble sugars, organic acid is decomposed, and the sweetness of the fruit is increased, and the sourness is reduced; the hydrolysis of pectin makes the cell wall thinner, the hardness of the fruit is reduced and soft, and the chlorophyll pigment is degraded , Anthocyanins and carotenoid pigments are synthesized in a large amount, so that the color of the peel changes from green to red or yellow. EFE (ethylene forming enzyme) is a key enzyme in ethylene biosynthesis. The researchers achieved the go...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/74C12N15/82A01H5/00A01H6/82
CPCC07K14/415C12N15/8261
Inventor 冯涛李爱
Owner TIANJIN AGRICULTURE COLLEGE
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