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Identification method and application of pathogenic bacteria of weedy rice

An identification method and technology for weedy rice, which are applied in the determination/inspection of microorganisms, biochemical equipment and methods, measuring devices, etc., can solve the increasingly serious damage to the yield and quality of cultivated rice, and remain in the theoretical and early stage of exploration. , rice yield reduction and other issues, to achieve the effect of benefiting agricultural development, increasing farmers' income and reducing harm

Pending Publication Date: 2019-12-24
INST OF CROP SCI NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Weedy rice, also known as wild rice and miscellaneous rice, is a global weed damage. Weedy rice associated with cultivated rice is widely distributed in many countries and regions in the world. Their morphological characteristics are between wild rice and cultivated indica and japonica rice. Weedy rice is very similar to wild rice. The mature seeds are light, dormant, and easy to shatter. The tillering and flowering stages are generally earlier than the associated cultivated rice varieties. Compared with cultivated rice, weedy rice competes for nutrients, The dominance of water and light will result in a reduction in rice yield and quality. In rice production, the yield reduction caused by weedy rice can generally reach 4.3% to 8.4%. In severe cases, the yield can be greatly reduced.
[0003] In recent years, weedy rice has begun to occur in transplanted rice areas in northern China, and its distribution has gradually expanded, causing more and more serious damage to the yield and quality of cultivated rice. Weedy rice is the same species as cultivated rice, but weedy rice belongs to the wild type , follow the law of natural survival in the process of survival and reproduction, and its phenotype, stress resistance and other characteristics are somewhat different from those of cultivated rice. Weed pathogenic fungi are natural enemies of weeds, and there have been many successful examples of pathogenic microorganisms controlling weeds , the biological herbicides developed by using fungi are called fungal herbicides. Although the method of using biotechnology to control weedy rice is still at the theoretical and early stage of exploration, the idea of ​​using biotechnology to control weedy rice has broad potential. Foreground, therefore, using weed pathogens to develop fungal herbicides to control the damage of weedy rice is a new idea. The most basic work in the research and development of fungal herbicides is the investigation of weed pathogens. Research studies have not yet been reported

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  • Identification method and application of pathogenic bacteria of weedy rice
  • Identification method and application of pathogenic bacteria of weedy rice
  • Identification method and application of pathogenic bacteria of weedy rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The invention provides a method for identification of weedy rice pathogenic bacteria, the specific method is as follows:

[0029] S1. Extensive collection of weedy rice disease specimens: collect diseased weedy rice specimens from rice planting areas in the whole region at different periods, and collect specimens that are easy to separate and can produce conidia strains according to stems, leaves, seeds, etc., and place them in Take it back in a fresh-keeping bag, store it in a refrigerator at 4°C and store it in a sterile air-dry;

[0030] S2. Isolation, purification and preservation of weedy rice pathogenic bacteria:

[0031] S2.1. Select the stems, leaves and seeds of the diseased plants, and take the junction of the diseased joints. After washing away the dirt with clean water, rinse it with sterile water several times in an ultra-clean workbench, and cut it with sterilized scissors. into small pieces of 0.25 square centimeters, soaked in 75% (v / v) alcohol for 10 s...

Embodiment 2

[0042] The invention provides a method for identification of weedy rice pathogenic bacteria, the specific method is as follows:

[0043] S1. Extensive collection of weedy rice disease specimens: collect diseased weedy rice specimens from rice planting areas in the whole region at different periods, and collect specimens that are easy to separate and can produce conidia strains according to stems, leaves, seeds, etc., and place them in Take it back in a fresh-keeping bag, store it in a refrigerator at 4°C and store it in a sterile air-dry;

[0044] S2. Isolation, purification and preservation of weedy rice pathogenic bacteria:

[0045] S2.1. Select the stems, leaves and seeds of the diseased plants, and take the junction of the diseased joints. After washing away the dirt with clean water, rinse it with sterile water several times in an ultra-clean workbench, and cut it with sterilized scissors. Form into small pieces of 0.4 square centimeters, soak in 75% (v / v) alcohol for 10...

Embodiment 3

[0056] The invention provides a method for identification of weedy rice pathogenic bacteria, the specific method is as follows:

[0057] S1. Extensive collection of weedy rice disease specimens: collect diseased weedy rice specimens from rice planting areas in the whole region at different periods, and collect specimens that are easy to separate and can produce conidia strains according to stems, leaves, seeds, etc., and place them in Take it back in a fresh-keeping bag, store it in a refrigerator at 4°C and store it in a sterile air-dry;

[0058] S2. Isolation, purification and preservation of weedy rice pathogenic bacteria:

[0059] S2.1. Select the stems, leaves and seeds of the diseased plants, and take the junction of the diseased joints. After washing away the dirt with clean water, rinse it with sterile water several times in an ultra-clean workbench, and cut it with sterilized scissors. Form into small pieces of 0.5 square centimeters, soak in 75% (v / v) alcohol for 10...

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Abstract

The invention discloses an identification method and application of pathogenic bacteria of weedy rice, and particularly relates to the technical field of identification of the pathogenic bacteria of the weedy rice. The method includes the specific steps: S1, widely collecting weedy rice disease samples; S2, separating, purifying and storing the pathogenic bacteria of the weedy rice; S3, identifying the pathogenic bacteria of the weedy rice; and S4, determining pathogenicity of the pathogenic bacteria of the weedy rice. According to the method, investigation and screening of pathogenic fungi ofthe weedy rice are actively carried out, so that a pathogenic fungus resource database of the weedy rice is established; fungus herbicides are developed advantageously, and can be applied to rice production to reduce the harm to the weedy rice, the safety of rice planting is guaranteed to the maximum extent, the economic benefit of rice farmers is improved, agricultural development and farmer income increase are further facilitated, and important scientific significance and wide application prospects are achieved.

Description

technical field [0001] The invention relates to the technical field of identification of weedy rice pathogenic bacteria, more specifically, the invention relates to an identification method and application of weedy rice pathogenic bacteria. Background technique [0002] Weedy rice, also known as wild rice and miscellaneous rice, is a global weed damage. Weedy rice associated with cultivated rice is widely distributed in many countries and regions in the world. Their morphological characteristics are between wild rice and cultivated indica and japonica rice. Weedy rice is very similar to wild rice. The mature seeds are light, dormant, and easy to shatter. The tillering and flowering stages are generally earlier than the associated cultivated rice varieties. Compared with cultivated rice, weedy rice competes for nutrients, The dominance of water and light will result in reduced rice yield and quality. In rice production, the yield reduction caused by weedy rice can generally r...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12Q1/04
CPCC12Q1/18C12Q1/04G01N2333/37
Inventor 史延丽陈东升刘炜黄新玲李文强沙蓉黄玉峰杨生龙蒲丽丽王彩芬王坚
Owner INST OF CROP SCI NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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