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Tilia miqueliana tissue culture method

A culture method and a technology for rooting tissue culture, applied in the field of Nanjing linden tissue culture, can solve problems such as weak reproductive ability, achieve the effects of simple cleaning and disinfection, simple seedling raising steps, and reducing the degree of dependence

Inactive Publication Date: 2019-12-24
ANHUI SHUCHENG JINQIAO AGRI & FORESTRY SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to various reasons such as regional selectivity and weak reproductive ability, Nanjing Tilia has become an endangered tree species under key protection.

Method used

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  • Tilia miqueliana tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Nanjing Tilia tissue culture method, comprises the following steps:

[0024] 1) Select explants: in fine weather in March of the lunar calendar, select the thick and tender shoots on the southeast side branches of the 2-year-old Nanjing Tilia as the first explants, and the first explants are 50mm; select 1-year-old The shoots of the semi-lignified Nanjing Tilia in the northwest direction were used as the second explants, and the second explants were 3 mm in diameter.

[0025] 2) Pretreatment: soak the first explant in alkaline detergent water for 5 minutes, and then rinse it under tap water for 1 hour. The pH of the alkaline cleaner used was 7.5. Brush the fluff on the surface of the second explant first with a brush, then soak it in a neutral detergent for 3 minutes, and then rinse it under tap water for 5 minutes. After cleaning, put the first explant and the second explant into an ultra-clean workbench respectively, wash them with sterile water for 3 times, wash th...

Embodiment 2

[0037] Nanjing Tilia tissue culture method, comprises the following steps:

[0038] 1) Select explants: in the fine weather in April of the lunar calendar, select the thick and tender shoots on the southeast side branches of the 2-year-old Nanjing Tilia as the first explants, and the first explants are 60mm; select 1-year-old The shoots of the semi-lignified Nanjing Tilia in the northwest direction were used as the second explants, and the second explants were 4 mm in diameter.

[0039]2) Pretreatment: soak the first explant in alkaline detergent water for 8 minutes, and then rinse it under tap water for 1.5 hours. The pH of the alkaline cleaner used was 8. Brush the fluff on the surface of the second explant first with a brush, then soak it in a neutral detergent for 4 minutes, and then rinse it under tap water for 8 minutes. After cleaning, put the first explant and the second explant into an ultra-clean workbench respectively, wash them with sterile water for 3 times, was...

Embodiment 3

[0051] Nanjing Tilia tissue culture method, comprises the following steps:

[0052] 1) Select explants: in fine weather in March of the lunar calendar, select the thick and tender shoots on the southeast side branches of the 2-year-old Nanjing Tilia as the first explants, and the first explants are 80mm; select 1-year-old The shoots of the semi-lignified Nanjing Tilia in the northwest direction were used as the second explants, and the second explants were 5 mm in diameter.

[0053] 2) Pretreatment: soak the first explant in alkaline detergent water for 10 minutes, and then rinse it under tap water for 2 hours. The pH of the alkaline cleaner used was 8.5. Brush the fluff on the surface of the second explant first with a brush, then soak it in a neutral detergent for 5 minutes, and then rinse it under tap water for 10 minutes. After cleaning, put the first explant and the second explant into an ultra-clean workbench respectively, wash them with sterile water for 5 times, wash...

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Abstract

The invention discloses a tilia miqueliana tissue culture method, including the steps of selecting explants, to be specific, taking a strong shoot on the southeast branch of two-year-old tilia miqueliana as the first explant, and taking a shoot on the northwest semi-ligneous branch of one-year-old tilia miqueliana tissue culture as the second explant; preprocessing; performing cuttage grafting byparticularly grafting the second explant to the lateral side of the first explant in regular manners, fastening the grafted portions with linen thread, and cutting 2-3 wedged cuts on the circumferenceof the first explant; and finally, cultivating. The method has the advantages that the plant of tilia miqueliana cultured by the method has high adaptability to territory, and is easy to be cared during growth.

Description

technical field [0001] The invention relates to the technical field of seedling cultivation, in particular to a Nanjing Tilia tissue culture method. Background technique [0002] Nanjing Tilia is a unique tree species in Nanjing, distributed in Jiangsu, Anhui, Zhejiang and other provinces. This kind of tree has beautiful shape, majestic posture, thick leaves, long life, fragrant flowers, great ornamental value, and strong resistance to smoke and gas. It is a good shade tree, street tree, and an excellent honey source tree species. The application prospect is broad. However, due to various reasons such as regional selectivity and weak reproductive ability, Nanjing Tilia has become an endangered tree species under key protection. Contents of the invention [0003] The object of the present invention is to provide a method for tissue culture of Nanjing Tilia, which has the characteristics that the cultivated Nanjing Tilia has strong adaptability to the region and is easy to...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G2/30A01G7/06
CPCA01H4/008A01G2/30A01G7/06A01H4/001
Inventor 丁增成袁应菊曹雨薇
Owner ANHUI SHUCHENG JINQIAO AGRI & FORESTRY SCI & TECH CO LTD
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