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Isolated culture and rapid propagation method of Ilex asprella

A technology of in vitro culture and gang plum, applied in the field of in vitro culture and rapid propagation of gang plum, can solve the problems of no factory production of gang plum, and achieve the effect of guaranteeing the supply market, promoting comprehensive utilization, and promoting multiplication

Inactive Publication Date: 2019-12-24
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some Chinese medicinal materials have also successfully established tissue culture technology systems, and some varieties have carried out industrialized seedling cultivation, but there is no report on industrialized production of Gangmei.

Method used

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  • Isolated culture and rapid propagation method of Ilex asprella
  • Isolated culture and rapid propagation method of Ilex asprella
  • Isolated culture and rapid propagation method of Ilex asprella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1 Materials and methods

[0028] 1.1 Materials

[0029] In the spring of 2018, the vigorously growing Gangmei seedlings in Guangzhou were cultivated in the Key Laboratory of Crop Genetic Improvement, Crop Research Institute, Guangdong Academy of Agricultural Sciences.

[0030] 1.2 Medium and culture conditions

[0031] Document 1 described in this embodiment is "Plant Tissue Culture Rapid Propagation Technology" edited by Qiu Yunliang and Duan Penghui published by Chemical Industry Press. Formulation of the original MS medium described in this example References 1 page 2-1MS medium (Murashige and Skoog, 1962); Section 7 on page 27 - preparation of culture medium.

[0032] The formulation of the improved MS medium: use Ca(NO 3 ) 2 4H 2 O instead of CaCl in the original MS medium 2 2H 2 O, add ferrous sulfate, add sucrose, add agar, other components have no change; wherein Ca(NO 3 ) 2 4H 2 The concentration of O is 95mg / L, the concentration of ferrous sulfate i...

Embodiment 2

[0087] A method for in vitro culture and rapid propagation of Gangmei, characterized in that the steps are as follows:

[0088] (1) Preparation of improved MS medium

[0089] The improved MS medium was prepared according to the formula of the improved MS medium as follows: with Ca(NO 3 ) 2 4H 2 O instead of CaCl in the original MS medium 2 2H 2 O, adding ferrous sulfate, adding sucrose, adding agar, no change in other components; where Ca(NO 3 ) 2 4H 2 The concentration of O is 100mg / L, the concentration of ferrous sulfate is 60mg / L, and the concentration of sucrose is 32g / L -1 , the concentration of agar is 6.0g / L -1 ;

[0090] (2) Disinfection of explants and induction of adventitious buds

[0091] Select the young and tender branches of Gangmei as explants, remove the leaves, and clean up the dust on the surface; on the ultra-clean workbench, cut the cleaned branches into stems with 1-2 buds, and place the stems at 70 Soak in % alcohol for 30s, rinse 3 times with...

Embodiment 3

[0100] A kind of in vitro culture rapid propagation method of Gangmei, the steps are as follows:

[0101] (1) Preparation of improved MS medium

[0102] The improved MS medium was prepared according to the formula of the improved MS medium as follows: with Ca(NO 3 ) 2 4H 2 O instead of CaCl in the original MS medium 2 2H 2 O, adding ferrous sulfate, adding sucrose, adding agar, no change in other components; where Ca(NO 3 ) 2 4H 2 The concentration of O is 90mg / L, the concentration of ferrous sulfate is 50mg / L, and the concentration of sucrose is 38g / L -1 , the concentration of agar is 5.0g / L -1 ;

[0103] (2) Disinfection of explants and induction of adventitious buds

[0104] Select the young and tender branches of Gangmei as explants, remove the leaves, and clean up the dust on the surface; on the ultra-clean workbench, cut the cleaned branches into stems with 1-2 buds, and place the stems at 70 Soak in % alcohol for 30s, rinse 3 times with sterile water, each ti...

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Abstract

The invention discloses an isolated culture and rapid propagation method of Ilex asprella. The method includes the steps: replacing CaCl2*2H2O in an MS medium by Ca(NO3)2*4H2O, adding ferrous sulfate,sucrose and agar to prepare an improved MS medium, and inoculating an explant of the Ilex asprella into the improved MS medium added with 6-BA and IBA to perform inducing culture; inoculating taken adventitious buds of the Ilex asprella into a cluster bud proliferation medium based on the improved MS medium and added with 6-BA, AD and NAA, transferring the adventitious buds to a rooting medium, and adjusting the dosage of a lot of elements in the improved MS medium as half of the original dosage, and adding activated carbon, NAA and IBA; finally, performing indoor acclimatization and transplantation on Ilex asprella seedlings to obtain the Ilex asprella. According to a tissue culture technique, a lot of Ilex asprella seedlings are supplied in a short term, the problem of progeny segregation is solved, a supply market is guaranteed, incomes of planters and sellers are improved, and comprehensive utilization of the Ilex asprella is facilitated.

Description

technical field [0001] The invention relates to a plant tissue culture rapid propagation technology, in particular to a rapid propagation method for in vitro culture of sage plums. Background technique [0002] Ilex asprella, also known as Gangmei, belongs to the Ilex genus of the Ilex family, and its roots (Ilex asprella) and leaves are mostly used as medicine; its roots are bitter, sweet, and cold in nature, and return to the lungs, liver, and large intestine. Classic, non-toxic, can clear away heat and detoxify, promote blood circulation, promote fluid production, and can cure colds, dizziness, headache, sore throat, whooping cough, gonorrhea and bruises. The leaves are bitter and sweet in nature, cold in nature, return to the heart, lung and liver channels, clear away heat and detoxify, and can cure colds and traumatic injuries. The leaves also contain ursolic acid, which has a therapeutic effect on angina pectoris and coronary heart disease. Gangmei is one of the raw ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王继华蔡时可谢梅新梅瑜
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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