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Method for detecting gypenoside A in gelan Xinning soft capsules by adopting HPLC-UV method

A technology of Gypenoside and Gypenoside, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor accuracy and cumbersome operation process, and achieve good durability, simple processing and detection process, and good accuracy Effect

Active Publication Date: 2019-12-20
XIAN CHIHO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the quality standard of total glycosides of Gypenoside, with Gypenoside A as a control, the content of total glycosides in the sample is determined by visible spectrophotometry, which is cumbersome and has poor accuracy

Method used

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  • Method for detecting gypenoside A in gelan Xinning soft capsules by adopting HPLC-UV method
  • Method for detecting gypenoside A in gelan Xinning soft capsules by adopting HPLC-UV method
  • Method for detecting gypenoside A in gelan Xinning soft capsules by adopting HPLC-UV method

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Embodiment 1

[0035] Embodiment 1: A kind of detection method adopting HPLC-UV method to measure gypenosides in Gelanxinning soft capsule comprises the following steps:

[0036] Step 1: Preparation of GLAXINING Soft Capsules and Negative Control Capsules

[0037] 1. Preparation of Granxinine Soft Capsules

[0038] Gelanxinning soft capsule prescription: 200g total flavonoids of kudzu root, 60g hawthorn extract, 20g gypenosides, 254g salad oil, 6g beeswax, 20g hydrogenated palm oil, 10g soybean lecithin, 0.5g methyl silicone oil, made into 1000 capsules.

[0039] Preparation method of Gelanxinning soft capsule: take salad oil, add beeswax and hydrogenated palm oil, heat to dissolve, let cool, add soybean lecithin, stir well, add kudzu root flavonoids, hawthorn extract, gypenosides, stir well, Grind the colloid evenly, add methyl silicone oil, decompress and defoam, and press the medicine to make soft capsules.

[0040] 2. Preparation of Negative Control Capsules

[0041] Gypenosides were ...

Embodiment 2

[0115] Embodiment 2: A method for detecting gypenoside A in Gelanxinning soft capsules by HPLC-UV method, characterized in that: after Gelanxinning soft capsules are pretreated, high performance liquid chromatography (HPLC- UV) measurement, that is, each Gelanxinning soft capsule contains gypenosides and gypenoside A (C 46 h 74 o 17 ), not less than 0.5mg, the specific steps are as follows:

[0116] (1) Preparation of the test solution: take 8 capsules of Glacicin soft capsules, add 90ml of 10% methanol, weigh them, reflux in a water bath for 1 hour, let cool, weigh again, add 10% methanol to reduce the weight. Lost weight, shake well, centrifuge;

[0117] After centrifugation, take the supernatant, discard the oil layer, weigh 23ml of the water layer, put it in a separatory funnel, shake and extract once with 23ml of methyl tert-butyl ether, discard the ether solution, and shake the water solution with n-butanol saturated with water. Shake and extract 3 times, 23ml each t...

Embodiment 3

[0122] Embodiment 3: a kind of method adopting HPLC-UV method to detect gypenoside A in Gelanxinning soft capsule, it is characterized in that: after Gelanxinning soft capsule is pretreated, directly adopt high-performance liquid chromatography (HPLC- UV) measurement, that is, each Gelanxinning soft capsule contains gypenosides and gypenoside A (C 46 h 74 o 17 ), not less than 0.5mg, the specific steps are as follows:

[0123] (1) The preparation of need testing solution: get Glandine soft capsule: 11, add 10% methyl alcohol 110ml, weigh, water-bath reflux, let cool, weigh again, supplement lost with 10% methanol weight, shake, centrifuge;

[0124] After centrifugation, take the supernatant, discard the oil layer, weigh 27ml of the water layer, put it in a separatory funnel, shake and extract twice with 27ml of methyl tert-butyl ether, discard the ether solution, and shake the water solution with n-butanol saturated with water. Shake and extract 5 times, 27ml each time, co...

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Abstract

The invention relates to a method for detecting gypenoside A in a gelan Xinning soft capsule by adopting an HPLC-UV method. The gelan Xinning soft capsule is subjected to pretreatment and is measuredby adopting a high performance liquid chromatograph (HPLC-UV), and the measurement result is that the gypenoside contained in each gelan Xinning soft capsule is calculated by gypenoside A (C46H74O17)and is not less than 0.5mg. The detection method is good in specificity, good in instrument precision, good in linearity, precision, accuracy and durability, simple, convenient and accurate in processing and detection processes, and suitable for detection application in production of the gelan Xinning soft capsules.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a method for detecting gypenoside A in Gelanxinning soft capsules by using an HPLC-UV method. Background technique [0002] Gelanxinning Soft Capsule is a traditional Chinese medicine produced by the applicant Xi'an Qianhe Pharmaceutical Co., Ltd. The clinical application in hospitals all over the country shows that it has significant clinical effects in the treatment of coronary heart disease and angina pectoris caused by congestion and obstruction. Preparation of Gelanxinning Soft Capsules: 200g of Pueraria total flavonoids, 60g of hawthorn extract, 20g of gypenosides, 254g of salad oil, 6g of beeswax, 20g of hydrogenated palm oil, 10g of soybean lecithin, 0.5g of methyl silicone oil, made into 1000 capsules ; Preparation method: take salad oil, add beeswax, hydrogenated palm oil, heat to dissolve, let cool, add soybean lecithin, stir well, add kudzu roo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/36G01N30/74G01N30/34G01N30/8679G01N2030/027
Inventor 胡江可张琼胡小虎谭祥和吕慧锋
Owner XIAN CHIHO PHARMA
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