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A drying method for hyaluronic acid

A drying method and technology of hyaluronic acid, applied in the field of biomacromolecule preparation, can solve the problems of difficult agglomeration, high water content of hyaluronic acid and its salts, and achieve the effects of enhancing stability, prolonging validity period and weakening hydrolysis reaction.

Active Publication Date: 2021-06-25
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of high water content of the current finished hyaluronic acid and its salt, the present invention provides a drying method for hyaluronic acid and its salt, the obtained product has low water content and is not easy to agglomerate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Add 2g of sodium hyaluronate (640,000 Daltons, loss on drying: 9.17%) to 500mL of normal saline to prepare a 4g / L sodium hyaluronate solution;

[0025] Prepare a mixture of hexamethyldisiloxane and absolute ethanol at a ratio of 5:95 (V / V) as organic solvent I containing 5% volatile silicone oil;

[0026] Slowly add 3 times the volume of organic solvent I to the sodium hyaluronate aqueous solution, and after standing still, discard the supernatant to obtain about 500 mL of precipitate;

[0027] (2) Prepare a mixture of hexamethyldisiloxane and absolute ethanol at a ratio of 50:50 (V / V) as organic solvent II containing 50% volatile silicone oil;

[0028] Take 2500mL of organic solvent II and add it to the precipitate in step (1), stir for 60 minutes, then let it stand and discard the supernatant to obtain the precipitate, repeat the above process 3 times, and filter the precipitate with filter cloth to obtain final sediment;

[0029] (3) Take the final precipitate ...

Embodiment 2

[0031] The experimental procedure is the same as in Example 1, except that the molecular weight is 150,000 Daltons, and the measured initial loss on drying of sodium hyaluronate is 7.09%; the measured loss on drying of the dried sodium hyaluronate obtained is 3.56%.

Embodiment 3

[0033] The experimental procedure is the same as that in Example 1, except that the molecular weight is 1.15 million Daltons, and the initial loss on drying of sodium hyaluronate is 10.67%; the dry weight loss on drying of the obtained sodium hyaluronate dry product is 4.27%.

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PUM

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Abstract

The invention provides a method for drying hyaluronic acid, which includes the following steps: adding organic solvent I containing volatile silicone oil to a solution containing hyaluronic acid, and after precipitating, discarding the supernatant; Wash with organic solvent II containing volatile silicone oil to obtain a precipitate, and obtain the product after drying. The volume fraction of the volatile silicone oil in organic solvent I is 1%-10%; the volume fraction in organic solvent II is 20%-60%. The hyaluronic acid drying method provided by the present invention is suitable for hyaluronic acid samples of different molecular weights and different concentrations. After drying using the method of the present invention, the weight loss on drying does not exceed 5%, and the organic solvent residues comply with regulations.

Description

technical field [0001] The invention belongs to the field of biomacromolecule preparation and relates to a drying method for hyaluronic acid and its salt. Background technique [0002] Hyaluronic acid (HA), also known as hyaluronic acid, is composed of (1-β-4) D-glucuronic acid and (1-β-3) N-acetyl-D-glucosamine disaccharide units Chain-like polyanionic mucopolysaccharide composed of repeated links. With its unique molecular structure and physical and chemical properties, hyaluronic acid shows a variety of important physiological functions in the body, such as lubricating joints, regulating the permeability of blood vessel walls, regulating protein, water and electrolyte diffusion and operation, and promoting wound healing. More importantly, hyaluronic acid has a special water-retaining effect, and is the most moisturizing substance found in nature so far, and is known as an ideal natural moisturizing factor. [0003] Hyaluronic acid is very easy to absorb moisture. At pre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/0072
Inventor 韩月梅李超杨盛林田晨颖刘建建郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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