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Method for extracting hydroxycitrate from garcinia cambogia fruits

A hydroxycitric acid and fruit technology, applied in the directions of carboxylate preparation, carboxylate preparation, chemical instruments and methods, etc., can solve problems such as the reduction of biological activity, reduce the formation of lactones, improve the purity and biological activity Effect

Active Publication Date: 2019-12-20
HUNAN UNIV OF HUMANITIES SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Hydroxycitric acid is an organic acid containing 2 hydroxyl groups and 3 free carboxyl groups. It is easy to condense into lactone during the separation and extraction process, which reduces the biological activity of the product.

Method used

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  • Method for extracting hydroxycitrate from garcinia cambogia fruits
  • Method for extracting hydroxycitrate from garcinia cambogia fruits
  • Method for extracting hydroxycitrate from garcinia cambogia fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] S1. Take Garcinia cambogia fruit powder that is crushed to 40 meshes;

[0035] S2. In step S1, add water according to the ratio of material-to-liquid ratio of 1:14, then cook under high pressure at 115°C for 15 minutes, after cooling, filter and squeeze out the filtrate with 5 layers of medical gauze, and centrifuge the filtrate at 4000×g for 15 minutes;

[0036] S3. Concentrate the supernatant by evaporation under reduced pressure at 55°C, the concentration factor is 5 times, and then add 95% ethanol to precipitate pectin in the concentrated solution at a ratio of 1:1 (v / v);

[0037] S4. Filter the mixture obtained in step S3 with gauze and centrifuge at 4000×g for 15 min to remove pectin. Add 95% ethanol to the supernatant and adjust it at room temperature with a 40-70 degree alcohol meter (Hebei Wuqiang Huapeng Instrument Factory) The alcohol content of the solution is up to 60;

[0038] S5. Then add 40% KOH to adjust the pH to 12;

[0039] S6. Discard the supernat...

Embodiment 2

[0041] S1. Take Garcinia cambogia fruit powder that is crushed to 30 meshes;

[0042] S2. In step S1, add water according to the ratio of material-to-liquid ratio of 1:15, then cook under high pressure at 110°C for 20 min, after cooling, filter and extrude the filtrate with 5 layers of medical gauze, and centrifuge the filtrate at 4000×g for 15 min;

[0043] S3. Concentrate the supernatant by evaporation under reduced pressure at 55°C, the concentration factor is 6 times, and then add 90% ethanol to precipitate pectin in the concentrated solution at 1:2 (v / v);

[0044] S4. Filter the mixture obtained in step S3 with gauze and centrifuge at 4000×g for 15 min to remove pectin. Add 95% ethanol to the supernatant and adjust it at room temperature with a 40-70 degree alcohol meter (Hebei Wuqiang Huapeng Instrument Factory) The alcohol content of the solution is up to 58;

[0045] S5. Then add 40% KOH to adjust the pH to 11.5;

[0046] S6. Discard the supernatant after the oily pr...

Embodiment 3

[0048] S1. Take Garcinia cambogia fruit powder that is crushed to 50 mesh;

[0049] S2. In step S1, add water according to the ratio of material-to-liquid ratio of 1:13, then cook under high pressure at 120°C for 10 minutes, after cooling, filter and squeeze out the filtrate with 5 layers of medical gauze, and centrifuge the filtrate at 4000×g for 15 minutes;

[0050] S3. Concentrate the supernatant by evaporation under reduced pressure at 55°C, the concentration factor is 4 times, and then add 90% ethanol to precipitate pectin in the concentrated solution at 1:1 (v / v);

[0051] S4. Filter the mixture obtained in step S3 with gauze and centrifuge at 4000×g for 15 min to remove pectin. Add 95% ethanol to the supernatant and adjust it at room temperature with a 40-70 degree alcohol meter (Hebei Wuqiang Huapeng Instrument Factory) The alcohol content of the solution is up to 62;

[0052] S5. Then add 40% KOH to adjust the pH to 12.5;

[0053] S6. Discard the supernatant after t...

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Abstract

The invention relates to the field of plant extraction, in particular to a method for extracting a hydroxycitrate from garcinia cambogia fruits. The method comprises the following steps: S1, pulverizing garcinia cambogia fruits; S2, adding water into the garcinia cambogia fruits pulverized in the S1 according to a material-liquid ratio of 1:(13-15), carrying out boiling at 110-120 DEG C for 10-20min, carrying out cooling, then carrying out filtering, and retaining the filtrate; S3, concentrating the filtrate in step S2 by 4-6 times, and adding 90-95% ethanol with 1-2 times of the volume of theconcentrated solution; S4, filtering the solution obtained in the S3, and adding 90-95% ethanol into the filtrate to adjust the alcohol content to 58-62%; S5, adding an alkali solution in the solution obtained in the S4, and adjusting the pH value of the solution to 11.5-12.5; and S6, filtering the solution obtained in the S5, and carrying out washing, evaporating and drying. Through the method,hydroxycitric acid is in an alkaline atmosphere rather than directly forms a neutral salt with alkaline substances. As the carboxyl cannot form carboxyl salts, the alkaline condition is not beneficialto an esterification reaction, so that formation of lactone is effectively reduced, and purity and biological activity of the hydroxycitrate are improved.

Description

technical field [0001] The invention relates to the field of plant extraction, in particular to a method for extracting hydroxycitrate from garcinia cambogia fruit. Background technique [0002] Garcinia Cambogia ( Garcinia cambogia L.) is a kind of Garciniaceae tree distributed in the subtropical region of South Asia. The peel of its fruit is rich in hydroxycitric acid, and the fruit has a sour taste. It is often used as food seasoning and traditional medicine in South Asian countries. Hydroxycitric acid is a strong competitive inhibitor of ATP citrate lyase, which can inhibit fat synthesis and promote glycogenogenesis, increase the utilization rate of pyruvic acid and lactic acid, and suppress appetite. Therefore, hydroxycitric acid is often used as an active ingredient in weight loss functional foods. Recent studies have shown that hydroxycitric acid can also inhibit the formation of calcium oxalate crystals in the kidney. [0003] Hydroxycitric acid is an organic aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/41C07C51/42C07C59/245
CPCC07C51/412C07C51/42C07C59/245
Inventor 胡一鸿金晨钟张雪娇倪弦之罗泽伟袁源李真
Owner HUNAN UNIV OF HUMANITIES SCI & TECH
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