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Method capable of preventing browning of stem segments of litsea coreana in tissue culture and culture medium thereof

A leopard skin camphor, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of inhibiting the growth and differentiation of explants, prone to browning in tissue culture, reducing tissue culture efficiency and the like , to achieve the effect of facilitating promotion, promoting exudation and reducing browning rate

Active Publication Date: 2019-12-20
CHONGQING ACADEMY OF FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its tissues contain a large amount of polyphenolic compounds, which makes it difficult to root when cutting seedlings
Therefore, the use of tissue culture and rapid propagation technology is one of the main methods to solve the artificial breeding of leopard skin. However, because the polyphenol oxidase in the leopard skin is activated, the phenolic compounds in the cells are aggregated into dark black macromolecules. Substances that are prone to browning in tissue culture can inhibit the growth and differentiation of explants, thereby reducing the efficiency of tissue culture and even leading to the death of explants

Method used

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  • Method capable of preventing browning of stem segments of litsea coreana in tissue culture and culture medium thereof
  • Method capable of preventing browning of stem segments of litsea coreana in tissue culture and culture medium thereof
  • Method capable of preventing browning of stem segments of litsea coreana in tissue culture and culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of anti-browning medium for tissue culture of Leopard camphora:

[0033] Each liter of anti-browning medium contains MS basal medium, 2mg / L6-BA, 0.1mg / LNAA, 30mg / L sucrose, 6.5mg / L agar, riboflavin, water is used as solvent, and the balance is water. Set up 8 treatments, add riboflavin with different concentration gradients in each treatment as shown in Table 1, each treatment has 6 parallels, 3 repetitions, no riboflavin is added to the control group, and the steps of preparing the medium can be conventionally prepared in this field to operate. The MS basal medium is configured according to the formula routinely used by those skilled in the art. The specific processing is shown in Table 1:

[0034] Table 1

[0035] processing 1 processing 2 processing 3 Process 4 Process 5 Process 6 Process 7 Process 8 Riboflavin mg / L 1 2 3 4 5 6 7 8

[0036] Adopt the culture medium prepared above to carry out the tissue cultu...

Embodiment 2

[0050] The preparation of anti-browning medium and anti-browning gel is as in Example 1.

[0051] Explant treatment: After cleaning the picked stems of Cinnamomum camphora, they are directly transferred to the aseptic operating table, and do not soak in 1‰ PVP solution at low temperature for 24 hours.

[0052] Explant disinfection: as in Example 1.

[0053] Anti-browning cultivation: as in Example 1.

[0054] Observe the growth status of the explants of Cinnamomum camphora, the judging criteria for the growth status: as in Example 1.

[0055] The browning rate of the explants of Cinnamomum camphora is counted, and the statistical method is as in Example 1.

[0056] Statistical browning rate and growth status are as shown in Table 3:

[0057] Table 3 Cinnamomum camphora explants growth status and anti-browning situation under the different treatments of embodiment 2

[0058]

[0059] From the experimental data in Table 3, it can be seen that after the anti-browning gel a...

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Abstract

The invention discloses a method capable of preventing browning of stem segments of litsea coreana in tissue culture and a culture medium thereof, and belongs to the technical field of tissue culture.The culture medium contains an MS basic culture medium, 8 mg / L riboflavin, 2 mg / L of 6-BA, 0.1 mg / L of NAA, 30 mg / L of sucrose, and 6.5 mg / L of agar. The method capable of preventing browning of thestem segments of the litsea coreana in the tissue culture includes the steps of explant treatment, explant disinfection, and browning-preventing culture, and can be adopted to effectively reduce the browning rate of the stem segments of the litsea coreana in the tissue culture and increase the proliferation coefficient.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for preventing browning of stem segments in tissue culture of camphor chinensis and a culture medium thereof. Background technique [0002] Litsea coreana Levl.var.lanuginose (Migo) Yang et P.H.Huang is a small tree belonging to the genus Zingiberaceae of Lauraceae, and is the main raw material plant for the ancient tea drink Eagle Tea in Southwest China. In recent years, because of its rich flavonoid antioxidants such as kaempferol and quercetin, Eagle tea has the effect of lowering blood sugar and blood lipids, and is purely natural, pollution-free, and unique in flavor. It has been favored by the market and performed relatively well. application value and economic value. [0003] Leopard skin camphor is a dioecious plant. The fruit grows long and is easy to be eaten by birds. Occasionally, the seeds naturally exist on the mother tree. The seeds contain substa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 朱恒星黄飞逸戴前莉卢敏黄馨
Owner CHONGQING ACADEMY OF FORESTRY SCI
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