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Composition and reagent kit for early cervical cancer detection

A composition and technology for cervical cancer, applied in the field of compositions for early cervical cancer detection, can solve the problems of high false negative and low specificity of detection means

Active Publication Date: 2019-12-13
北京鑫诺美迪基因检测技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a high-sensitivity and high-specificity early cervical cancer detection primer composition, covering more than 98% of different types of cervical cancer, overcoming the problems of high false negative or low specificity of existing detection methods, as Auxiliary reagents for early diagnosis of cervical cancer

Method used

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  • Composition and reagent kit for early cervical cancer detection
  • Composition and reagent kit for early cervical cancer detection
  • Composition and reagent kit for early cervical cancer detection

Examples

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Embodiment 1

[0075] Example 1 Detection test of the kit for detecting the methylation of cervical cancer-related genes EPB41L3 and JAM3 genes

[0076] The components of the kit are listed in Table 1 below:

[0077] Table 1

[0078]

[0079] 162 cervical exfoliated cell samples with known pathological results were selected: 102 samples were identified as cervical cancer samples; 60 samples were low-grade intraepithelial lesion (CIN1) samples.

[0080] 1. Cell DNA was extracted from the above-mentioned 162 cases of cervical exfoliated cell samples. After the extraction was completed, the sample concentration and OD260 / 280 were measured using a Nanodrop-300 micro-spectrophotometer, and the OD260 / 280 was between 1.8 and 2.0.

[0081] 2. After the extraction of cellular DNA is completed, bisulfite conversion is performed on the extracted DNA, and the unmethylated cytosine (C) is converted into uracil (U), while the methylated cytosine (C) remains unchanged. Purified bis-DNA was obtained. ...

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Abstract

The invention discloses a composition and reagent kit for early cervical cancer detection. The composition comprises 3 CpG island regions subjected to high methylation in an EPB41L3 gene and 3 CpG island region amplifying primers subjected to high methylation in a JAM3 gene, 98% or above of different types of cervical cancer can be covered, and detection of polygene polymethylation regions can berealized through a one-pipe reaction and detection. A specific MGB probe and a degenerate closed primer are preferably selected for cooperation, so that sensitivity and accuracy are further increased.The whole detection process is free from wounds, simple and convenient to operate and easy to interpret, universal qPCR equipment can meet detection, a wholly-closed form is adopted in the PCR process, and the possibility of cross-contamination can be avoided; besides, three segments of two markers are detected at the same time, so that the result is more accurate; and all the factors are integrated, so that the composition disclosed by the invention has social promotion properties. Due to high sensitivity of detection, the composition is suitable for non-invasive early cervical cancer screening.

Description

technical field [0001] The invention relates to the field of nucleic acid in vitro diagnosis, in particular to a composition for early cervical cancer detection, which can be used for early screening and auxiliary diagnosis of cervical cancer by detecting specific gene methylation. Background technique [0002] Cervical cancer is the fourth most common cancer among women in the world, with approximately 567,600 new cases in 2018, and the fourth leading cause of death, with approximately 315,000 deaths in 2018. In China, cervical cancer is currently the fifth most common cancer among women, with 106,430 new cases in 2018, and it is the third most deadly cancer among women aged 15-44. [0003] Human papillomavirus (HPV, full name human papillomavirus) is the culprit of most cervical cancers, and the genomic DNA of HPV can be identified in about 95% of malignant cervical lesions. The cumulative risk of HPV infection in a person's life is as high as 80%, but most HPV infections...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6886C12Q2600/154C12Q2600/166C12Q2531/113C12Q2537/143C12Q2563/107C12Q2523/125C12Q2545/101
Inventor 刘沛王林海
Owner 北京鑫诺美迪基因检测技术有限公司
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