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Marker for distinguishing gingival stem cells and deciduous tooth pulp stem cells and application thereof

A dental pulp stem cell and stem cell technology, which is applied in the field of stem cells to achieve the effects of high specificity and sensitivity, low price and suitable for popularization and application

Active Publication Date: 2019-12-13
KYBIOSTEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No one has yet used it to differentiate gingival stem cells from deciduous dental pulp stem cells

Method used

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  • Marker for distinguishing gingival stem cells and deciduous tooth pulp stem cells and application thereof
  • Marker for distinguishing gingival stem cells and deciduous tooth pulp stem cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Screening of Identification Markers for Gingival Stem Cells and Dental Pulp Stem Cells of Deciduous Teeth

[0031] 1. Extraction of total RNA from gingival stem cells and deciduous pulp stem cells

[0032] In this experiment, gingival stem cell samples and corresponding dental pulp stem cell samples from five different groups of individuals were used.

[0033] Before the experiment, treat the operating environment with RNA inhibitors.

[0034] In the following operations, wear a mask, take the contents of the Erlenmeyer flask containing pipette tips, centrifuge tubes, etc. horizontally, and then immediately cover the bottle mouth to avoid environmental pollution.

[0035] 1) Pour off the medium in the culture dish / bottle, wash with PBS 2-3 times, add 0.7mL Trizol solution (red), blow off the cells attached to the culture dish / bottle with a gun, and transfer directly to a 1.5mL centrifuge Then add 0.5mL Trizol to the petri dish to wash the petri dish to harve...

Embodiment 2

[0060] Example 2 further verifies the application of ANXA2 in the identification of gingival stem cells and deciduous dental pulp stem cells

[0061] In order to further confirm whether ANXA2 can reliably distinguish gingival stem cells from deciduous tooth pulp stem cells, we expanded the sample group, and in the cell samples of 20 kinds of gingival stem cells and 20 kinds of deciduous tooth pulp stem cells different from Example 1, according to The method in Example 1 again carried out the detection of the expression level of ANXA2 and the expression level of GAPDH and the calculation of the expression value. The results showed that the expression level of ANXA2 in gingival stem cells was significantly higher than that in deciduous dental pulp stem cells (P<0.01).

[0062] Further, another 10 groups of samples were used for testing, and each group of samples contained 1 gingival stem cell sample and 1 deciduous dental pulp stem cell sample. Without telling them whether they...

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Abstract

The invention provides a marker for distinguishing gingival stem cells and deciduous tooth pulp stem cells and a distinguishing method thereof. According to the method, ANXA2 is used as the marker. The identification method is convenient and quick, is low in cost, has a relatively high reference value for distinguishing of the gingival stem cells and the deciduous tooth pulp stem cells and dentaltissue engineering, and is suitable for promotion and application.

Description

technical field [0001] The invention belongs to the field of stem cells, and in particular relates to a marker for distinguishing gingival stem cells and deciduous dental pulp stem cells and a method for distinguishing them. Background technique [0002] In recent years, with the deepening of research, a variety of oral stem cell subsets have been identified, such as gingival stem cells, deciduous dental pulp stem cells, apical papilla stem cells, periodontal ligament stem cells, etc. However, due to the different sources of oral stem cells, the quality of the cells has great heterogeneity; therefore, one of the key and difficult points of research at home and abroad in recent years is how to deeply understand the surface markers of various stem cell subpopulations derived from the oral cavity, And how to quickly and accurately distinguish and screen functional subgroups. [0003] Gingival stem cells and deciduous dental pulp stem cells have similar growth and differentiati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6876C12Q1/6851G01N33/68
CPCC12Q1/6876C12Q1/6851G01N33/68C12Q2600/166G01N2333/4703C12Q2531/113C12Q2563/107
Inventor 雷童张晓霜陈鹏杜宏武
Owner KYBIOSTEM CO LTD
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