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Environmentally-friendly biological microbial inoculum for repairing soil pollution and preparation method of environmentally-friendly biological microbial inoculum for repairing soil pollution

A biological agent and soil restoration technology, applied in the field of soil restoration, can solve the problems of soil biological flora destruction, structural stickiness, and destruction of microbial flora structure, so as to increase the number of viable bacteria, improve water retention, and promote reproduction Effect

Active Publication Date: 2019-12-13
NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extensive use of chemical fertilizers, chemical pesticides, and continuous cropping for many years during agricultural planting, the soil biological flora has been destroyed, and the process of microbial transformation of organic and inorganic substances has been destroyed, resulting in The ability of microorganisms to degrade harmful substances becomes lower, and the ecological environment of the biological flora is destroyed, which leads to the fact that heavy metals cannot be degraded by biological means after entering the soil, exceeding the carrying capacity of the soil itself and causing soil pollution
[0003] For heavy metal pollution, many use in-situ or ex-situ remediation technology, in-situ application of lime, zeolite, phosphate, sulfide, biochar and organic fertilizers to quickly repair the contaminated soil, or guest soil, leaching, electrodynamics Restoration and other ectopic repairs, these methods have achieved results to a certain extent, but many technologies need to overcome certain conditions to produce good results due to limitations such as high cost, easy to produce secondary pollution, and limited use
In the prior art, the in-situ remediation technology with passivating agents as the core is mostly used to degrade heavy metals in soil. These passivating agents have a single effect in the soil, and are mainly specially prepared for the degradation of heavy metals, but cannot fundamentally improve the damage. The ecological environment of microorganisms in the soil leads to a single effect, which eventually makes the passivator take a long time to degrade and maintain the effect of purifying heavy metals in the soil for a short time. agent
In addition, the in-situ remediation technology used in the prior art is mainly for remediation of acidic soil and neutral soil, while in alkaline soil, due to the deterioration of soil physical properties, the structure is sticky, the aeration is poor, and the activity of aerobic microorganisms in the soil is poor. , destroying the structure of the microbial flora in the soil, resulting in heavy metals entering the soil that cannot be degraded in time and causing pollution damage. When the passivating agent in the prior art is used to repair alkaline soil, the problem of single effect is more obvious

Method used

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  • Environmentally-friendly biological microbial inoculum for repairing soil pollution and preparation method of environmentally-friendly biological microbial inoculum for repairing soil pollution

Examples

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Embodiment 1

[0029] An environmentally friendly biological agent for remediating soil pollution, mainly comprising the following raw material components in parts by weight: 40 parts of mixed chitosan gel, 8 parts of Acetobacter xylinum, 10 parts of Trichoderma and 12 parts of modified microspheres.

[0030] A method for preparing an environmentally friendly biological agent for repairing soil pollution, the method for preparing an environmentally friendly biological agent for repairing soil pollution mainly includes the following preparation steps:

[0031](1) Mix cholesterol and pyridine in a beaker at a mass ratio of 1:25, and add succinic anhydride 0.8 times the mass of cholesterol to the beaker, and stir for 6 hours at a temperature of 60°C and a speed of 300r / min. , the material in the beaker was concentrated by rotary evaporation for 5 hours at a temperature of 80°C, a rotational speed of 120r / min, and a pressure of 600kPa to obtain a crude product, which was recrystallized in an eth...

Embodiment 2

[0037] An environmentally friendly biological agent for remediating soil pollution, mainly comprising the following raw material components in parts by weight: 40 parts of chitosan gel, 8 parts of Acetobacter xylinum, 10 parts of Trichoderma and 12 parts by weight modified microspheres.

[0038] A method for preparing an environmentally friendly biological agent for repairing soil pollution, the method for preparing an environmentally friendly biological agent for repairing soil pollution mainly includes the following preparation steps:

[0039] (1) Mix cholesterol and pyridine in a beaker at a mass ratio of 1:25, and add succinic anhydride 0.8 times the mass of cholesterol to the beaker, and stir for 6 hours at a temperature of 60°C and a speed of 300r / min. , the material in the beaker was concentrated by rotary evaporation for 5 hours at a temperature of 80°C, a rotational speed of 120r / min, and a pressure of 600kPa to obtain a crude product, which was recrystallized in an e...

Embodiment 3

[0045] An environmentally friendly biological agent for remediating soil pollution, mainly comprising the following raw material components in parts by weight: 40 parts of mixed chitosan gel, 8 parts of Acetobacter xylinum, 10 parts of Trichoderma and 12 parts microspheres.

[0046] A method for preparing an environmentally friendly biological agent for repairing soil pollution, the method for preparing an environmentally friendly biological agent for repairing soil pollution mainly includes the following preparation steps:

[0047] (1) Mix cholesterol and pyridine in a beaker at a mass ratio of 1:25, and add succinic anhydride 0.8 times the mass of cholesterol to the beaker, and stir for 6 hours at a temperature of 60°C and a speed of 300r / min. , the material in the beaker was concentrated by rotary evaporation for 5 hours at a temperature of 80°C, a rotational speed of 120r / min, and a pressure of 600kPa to obtain a crude product, which was recrystallized in an ethanol soluti...

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Abstract

The invention discloses an environmentally-friendly biological microbial inoculum for repairing soil pollution and a preparation method of the environmentally-friendly biological microbial inoculum for repairing soil pollution, and belongs to the technical field of soil repairing. The preparation method comprises the steps of modifying glucan, coating 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimidehydrochloride and N-hydroxy succinimide with the modified glucan and polylactic acid so as to obtain modified microspheres; then mixing chitosan with agarose, and adding the modified microspheres soas to obtain semi-finished products; and finally, mixing the semi-finished products with a bacterial culture solution, adding trichoderma and acetobacter xylinum, performing mixed culture, and performing filtration so as to obtain the environmentally-friendly biological microbial inoculum for repairing soil pollution. After the prepared environmentally-friendly biological microbial inoculum for repairing soil pollution is used, the content of viable counts in soil can be effectively increased, and the content of effective state heavy metal ions in the soil is reduced.

Description

technical field [0001] The invention relates to the technical field of soil remediation, in particular to an environment-friendly biological bacterial agent for remediating soil pollution and a preparation method thereof. Background technique [0002] At present, according to the survey of relevant national departments, soil damage is mainly caused by pollution, while land pollution is mainly concentrated in heavy metal pollution, such as: cadmium, chromium, lead, mercury and other heavy metals, and cadmium pollution is more common. The soil is a complex of organic and inorganic combinations, which is the basis of agricultural planting, and organic fertility is the key factor for the quality assurance of agricultural production. Because the soil itself has a variety of organic and inorganic nutrients and microbial groups composed of a variety of microorganisms , provide a good growth environment for the growth of crops through the interaction of microbial populations and nut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12R1/02C12R1/885B09C1/10B09C1/00
CPCB09C1/10B09C1/105B09C2101/00C12N11/10C12N11/096
Inventor 吴昊
Owner NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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