Application of trimethylamine oxide as biomarker of cerebral apoplexy
A technology of trimethylamine oxide and stroke, applied in the field of biomedicine, can solve the problems such as the relationship between TMAO and the risk of stroke, and the risk of cardiovascular events that are not further confirmed, and achieve the effect of reducing medical costs and preventing stroke.
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Embodiment 1
[0021] Example 1: Effect of TMAO level on mouse vascular endothelial function
[0022] Experimental method: 20 8-week-old healthy female C57BL / 6J mice (purchased from the Animal Experiment Center of the Third Military Medical University) were fasted overnight and divided into a blank group and a choline group, and the choline group was given a single intragastric administration Choline (choline, C7017 was purchased from Sigma, USA, 400mg / kg), the blank group was given the same dose of normal saline, and blood samples were collected from the tail vein 4 hours after gavage, and the plasma was separated by centrifugation and stored at -80°C for use . The blank group and the choline group were given normal saline and choline by intragastric administration every day, and blood samples were collected from the tail vein after 8 weeks, and the plasma was separated by centrifugation and stored at -80°C until use.
[0023] Detection method: LC / MS analysis was used to detect the level o...
Embodiment 2
[0032] Example 2. Effect of TMAO level on apoE- / -mice aortic atherosclerosis
[0033] Experimental method: 16 8-week-old healthy male apoE- / - mice (purchased from the Experimental Animal Center of Peking University Health Science Center) were randomly divided into a blank group and a choline group, and the choline group was given choline (choline, C7017 was purchased from Sigma, USA, 400mg / kg). The blank group was given the same dose of normal saline, and the blank group and the choline group were given normal saline and choline by gavage every day. They were killed after 16 weeks of administration, and blood was collected from the orbit.
[0034] Detection method: MDA was determined by thiobarbituric acid method, continuous paraffin sections of the aortic root, 5um, 40 pieces, one piece was taken every 5 pieces, a total of 8 sections were taken for HE staining, and AS plaque was determined by computer pathological image analysis software Block size; streptomycin and biotin co...
Embodiment 3
[0040] Example 3: A nested case-control study on the relationship between serum TMAO level and stroke risk (one) method
[0041] The study period was from 2008 to 2013, and the selection criteria for patients (subjects) were: 1) aged 45 to 75 years (inclusive), male or female; 2) meeting the diagnostic criteria for essential hypertension, excluding secondary hypertensive Blood pressure; 3) No history of stroke or myocardial infarction; 4) Voluntary participation and signed informed consent. The number of cases was 20,702, of which the enalapril folic acid group (group A) and the enalapril group (group B) each accounted for half, and the patients in the two groups took "Yiye" (10mg / 0.8mg) or enalapril Li tablets (10mg) were taken once a day, 1 tablet each time, and were taken continuously for 4.5 years (median). The primary efficacy indicator (primary endpoint) was the first symptomatic stroke (ischemic or hemorrhagic, excluding subarachnoid hemorrhage), and the secondary effi...
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