Sample purification and concentration device

Abstract

The invention discloses a sample purification and concentration device which comprises an end cover, a trough pipe, a filter membrane support, a filter membrane and a centrifugal pipe, a feed liquid runner is arranged on the inner side of the filter membrane support; the runner outlet is straight to the bottom, the trough pipe is provided with a sample accommodating cavity; a liquid outlet communicated with the sample accommodating cavity is formed outside the side of the trough pipe; the filter membrane is fixed on the outer side of the trough pipe and covers the liquid outlet, the filter membrane support is vertically or obliquely fixed on the trough pipe after being tightly attached to the filter membrane, then the filter membrane support and the trough pipe are sleeved into the centrifugal pipe together, and the end cover is tightly covered, the sample containing cavity, the filter membrane, the feed liquid flow channel and the centrifugal pipe are sequentially communicated to forma purification and concentration channel. The device is used for separating, cleaning, purifying, filtering, desalting and concentrating a sample under the action of driving force or centrifugal force; in the operation process, other media are not needed, other pipetting devices are not needed in the operation process, any sample loss is avoided, and a very small number of samples can be separated, purified, concentrated and the like.

Description

technical field [0001] The invention relates to a sample purification and concentration device, which is mainly used in the field of biological products such as separation, purification, concentration and desalination. Background technique [0002] Centrifugal filtration devices are mainly used for separation, cleaning, concentration, desalination and purification of biological product samples, such as urine, diagnostic reagents, monoclonal antibodies, plasma, spinal fluid, viruses, etc., with small processing capacity and high concentration multiples , the separation of samples, the speed of filtration and purification process and the recovery of residual samples have extremely important scientific research value for scientific research. However, the currently commercially available filter centrifuge tubes have low concentration after purification and concentration, and have a large sample loss, which is difficult to meet the high requirements of clinical and laboratory, es...

AI Technical Summary

Problems solved by technology

[0004] However, the conventional ultrafiltration centrifuge tubes currently available in the market have various defects. There is a certain inclination between the filter membrane and the feed liquid. The centrifugal filtration is a dead-end filtration, which is very easy to form concentration polarization. Accumulation on the surface of the membrane makes it difficult to concentrate. At the same time, it is easy to lead to long filtration time, low protein recovery rate, and the product is often easily damaged. At the same time, the products on the market are manufactured in multiple processes. The filter membrane is first fixed on the support plate. Then it is connected with the casing, which will easily cause membrane damage during the production process, the product qualification rate is low, and the price is high; therefore, it is urgent to increase the filtration speed, simplify the production process, and improve the product qualification rate to reduce production costs.

[0005] The patent notification number is CN206924902U discloses a purification and concentration device, after the filter membrane of the device is fixed on the inside of the filter plate, it is vertically or obliquely fixed on the side wall of the filter membrane support or horizontally fixed on the bottom of the filter membrane support; The membrane is fixed on the filter membrane support, and then they are welded on the trough tube, the membrane is easy to break, and the yield is low; the feed liquid of the trough tube passes through the membrane and enters the trough of the support plate, and the outlet is in the trough On the back, and it is a pinhole hole, it is very difficult to process

[0006] In the current commercially available purification and concentration devices, there is a certain inclination angle between the membrane and the feed liquid. Under the action of an external driving force, molecules larger than the membrane pore size cannot pass through the membrane pores, and are easy to accumulate in the membrane pores and block the membrane pores, resulting in slow concentration. , until the membrane pores are completely blocked, resulting in the failure of separation and concentration

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