Application of hemoglobin and hemoglobin derivatives in preparation of injection pharmaceutical preparations for resisting anemia

A technology for hemoglobin and pharmaceutical preparations, applied in the field of biomedicine, can solve the problems of increased risk of thrombosis and cardiovascular events, ineffective treatment, adverse reactions, etc., and achieve the effect of improving the concentration of hemoglobin and the number of red blood cells, with significant effects and restoring functions.

Pending Publication Date: 2019-12-03
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ESA has a good effect in clinical anemia treatment, but ESA also has obvious adverse reactions, and its common side effects include joint pain, rash, vomiting and headache, and ESA treatment may lead to increased risk of thrombosis and cardiovascular events
Research shows that high concentrations of ESA can lead to off-target phenomenon, resulting in ineffective treatment

Method used

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  • Application of hemoglobin and hemoglobin derivatives in preparation of injection pharmaceutical preparations for resisting anemia
  • Application of hemoglobin and hemoglobin derivatives in preparation of injection pharmaceutical preparations for resisting anemia
  • Application of hemoglobin and hemoglobin derivatives in preparation of injection pharmaceutical preparations for resisting anemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Firstly, cyclophosphamide (CTX) was used to establish chemotherapeutic anemic mice. The specific modeling plan was intraperitoneal injection of CTX 100mg / kg on day 1-3, intraperitoneal injection of CTX 150mg / kg on day 4, and intraperitoneal injection of CTX 200mg on day 5. / kg. After completing the preparation of the chemotherapy anemia model, the mice were randomly divided into groups of 8, and the experimental group was divided into five groups. / kg dose of porcine hemoglobin (pHb) was injected intraperitoneally for seven consecutive days, and the ESA group injected with a dose of 150U / kg per day was used as a positive control group, the model natural recovery group was used as a negative control group, and normal mice were injected with an equal volume of normal saline in the Normal group. Blood samples were taken 24 hours after the last injection.

[0046] Detection method:

[0047] 1.1 Determination of the number of hemoglobin and red blood cells: Blood was colle...

Embodiment 2

[0056] Firstly, adenine was prepared into a suspension with deionized water, and 250 mg / (kg.d) was continuously fed to rats for 6 weeks to obtain an anemia model of chronic kidney disease (CKD). After completing the CKD model preparation, the rats were randomly divided into groups of 8, and the experimental group was divided into five groups. Intravenous injection of polymerized porcine hemoglobin (pPolyHb) at a dose of kg for 21 consecutive days, daily injection of 150U / kg of ESA group as a positive control, model natural recovery group as a negative control group, normal rats injected with an equal volume of normal saline . Blood samples were taken 24 hours after the last injection.

[0057] Detection method:

[0058] 1.1 Determination of the number of hemoglobin and red blood cells: blood was collected from the eye socket of the rat after the last treatment, anticoagulant was added for anticoagulation, and the automatic five-classification multi-species blood analyzer was...

Embodiment 3

[0066] First, cyclophosphamide (CTX) was used to establish chemotherapeutic anemic mice. The specific modeling plan was intraperitoneal injection of CTX 100 mg / kg on day 1-3, intraperitoneal injection of CTX 150 mg / kg on day 4, and intraperitoneal injection of CTX 200 mg / kg on day 5. kg. After completing the preparation of the chemotherapy anemia model, the mice were randomly divided into groups of 8, and the experimental group was divided into six groups, which were injected with bovine hemoglobin (0.07mg / kg), bovine methemoglobin (0.8mg / kg), human carbon oxygen Hemoglobin (5mg / kg), canine deoxygenated hemoglobin (15mg / kg), double aspirin cross-linked sheep hemoglobin (40mg / kg), chicken sulphated hemoglobin (20mg / kg) and polymerized bovine hemoglobin mixture (20mg / kg), continuous injection for seven days The ESA group injected with 150U / kg per day was used as the positive control group, the model natural recovery group was used as the negative control group, and the normal mi...

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Abstract

The application discloses application of hemoglobin and hemoglobin derivatives in preparation of injection pharmaceutical preparations for resisting anemia. When an organism is in an anemia state, a small dosage, being 0.05 to 60 mg / kg / day, of hemoglobin or hemoglobin derivative is dosed through an abdominal cavity or veins or muscles, so that the hemoglobin concentration of the organism and the number of red blood cells are remarkably increased and the kidney function is enhanced. The injection dosage and the injection period are adjusted according to the actual conditions of people or animals until the organism are recovered to non-anemia states. The injection preparation also has a partial recovery effect on the damaged function of the kidney.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a new medical application of hemoglobin (Hb) and hemoglobin derivatives. Background technique [0002] Natural hemoglobin usually plays the role of carrying oxygen and releasing oxygen in red blood cells to supply the body with oxygen. Hemoglobin has a molecular weight of 64.5KD and contains four subunits, two α subunits and two β subunits. Each subunit in Hb is composed of a peptide chain and a heme (heme) molecule, and there is a ferrous iron on each heme molecule. Hemoglobin containing divalent iron is called ferrous hemoglobin, which has the function of carrying oxygen and releasing oxygen, and ferrous hemoglobin combined with oxygen is called oxyhemoglobin (O 2 Hb), ferrous hemoglobin without oxygen is called deoxygenated hemoglobin; ferrous hemoglobin is easily converted into other forms of hemoglobin, such as methemoglobin (metHb) (hemoglobin in which ferrous iron ...

Claims

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Application Information

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IPC IPC(8): G01N33/72G01N33/531
CPCG01N33/72G01N33/531
Inventor 严坤平
Owner NORTHWEST UNIV
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