Stem cell growth factor ethosome containing penetration enhancer peptide and preparation method thereof

A stem cell factor and growth factor technology, which is applied in the field of stem cell growth factor ethosomes containing penetration-promoting peptides and its preparation, can solve the problems of harsh storage conditions, inability to store for a long time, and loss of biological activity of stem cell factors, so as to improve permeability Skin absorption effect, simple preparation method, effect of reducing oxidation degree

Inactive Publication Date: 2019-12-03
陕西中鸿科瑞再生医学研究院有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

Moreover, the storage conditions of stem cell factors are harsh and cannot be stored at 4°C for a long time, otherwise the biological activity will be lost

Method used

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  • Stem cell growth factor ethosome containing penetration enhancer peptide and preparation method thereof
  • Stem cell growth factor ethosome containing penetration enhancer peptide and preparation method thereof
  • Stem cell growth factor ethosome containing penetration enhancer peptide and preparation method thereof

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Embodiment Construction

[0029] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0030] The embodiments of the present invention provide a stem cell growth factor ethosome containing a penetration-promoting peptide and a preparation method thereof, comprising the following steps:

[0031] (1) Primary isolation and culture of umbilical cord stem cells:

[0032] In the ultra-clean workbench, cut the umbilical cord into small pieces of about 5 cm, wash the residual blood on the surface of the umbilical cord with PBS; into 2mm 3 Left and right tissue pieces; transfer the shredded tissue to a 50mL centrifuge tube, centrifuge at 300g for 10min; carefully discard the upper culture medium, add a mixed digestive enzyme equal to the volume of the tissue, and shake and digest in a shaker at 37°C for 2h. The mixed digestive enzymes contain 2g / L type II collagenase, 0.8g / L neutral protease, and 0.03g / L hyaluronidase; centrifuge...

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Abstract

The invention relates to a stem cell growth factor ethosome containing penetration-promoting peptide and a preparation method thereof, which belong to the technical field of biomedical preparations, and the preparation method specifically comprises the following steps: collecting a stem cell culture supernate, and enriching stem cell secretion factors as a water phase; dissolving phospholipid, cholesterol and vitamin E with an alcoholic solution to obtain an oil phase; mixing the two phases to obtain a stem cell factor ethosome solution; homogenizing and unifying the particle size, and then mixing with a permeability-promoting peptide to obtain the stem cell growth factor ethosome containing the permeability-promoting peptide. The permeability-promoting peptide has the effect of further improving the permeability capacity of the ethosome to enable the ethosome to reach the deep layer of skin tissues, so that the curative effect of stem cell factors wrapped by the ethosome is enhanced.The ethosome produced according to the invention has the advantages of stable property, higher encapsulation efficiency and simple technological process, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of biomedical preparations, and discloses a stem cell growth factor ethosome containing a penetration-promoting peptide and a preparation method thereof. Background technique [0002] Transdermal drug delivery is a drug delivery method in which drugs are smeared or applied on the skin surface. Compared with oral or injection administration, transdermal administration has the advantages of convenient use, less medication frequency, avoiding gastrointestinal irritation and liver first-pass effect, maintaining stable and long-lasting blood drug concentration, reducing drug toxicity and side effects, and improving safety and efficacy, etc. It has many advantages, so the market prospect is broad, and it is listed as the three major drug delivery systems along with oral administration and injection. However, due to the existence of the stratum corneum of the skin, the absorption of drugs will be greatly restricte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K35/51A61K47/42A61P17/02
CPCA61K9/0014A61K9/1271A61K35/51A61K47/42A61P17/02
Inventor 王哲明磊国王清霞李阿峰
Owner 陕西中鸿科瑞再生医学研究院有限公司
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