Parathyroid hormone kit for detecting peripheral blood, and preparation method and application thereof

A parathyroid hormone and kit technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of large demand for blood samples, difficulty in repeated extraction, cumbersome operation, etc., and achieve short sample processing time and excellent accuracy and precision, the effect of simple preparation

Inactive Publication Date: 2019-11-22
BEIJING ELCOTEQ BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the commonly used methods for clinical detection of parathyroid hormone include chemiluminescence, colloidal gold immunochromatography, enzyme-linked immunoassay, and electrochemiluminescence. Most of these detection methods need to process the collected whole blood samples, which is time-consuming. Long, cumbersome operation, the blood samples used are in large demand and all are venous blood
However, for infants, children, and patients with extensive burns, it is difficult to collect enough blood samples and it is not easy to repeatedly draw blood samples.

Method used

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  • Parathyroid hormone kit for detecting peripheral blood, and preparation method and application thereof
  • Parathyroid hormone kit for detecting peripheral blood, and preparation method and application thereof
  • Parathyroid hormone kit for detecting peripheral blood, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the preparation of parathyroid hormone (PTH) kit

[0042] 1.1 Preparation of parathyroid hormone (PTH) immunochromatographic detection card

[0043] 1) Another strain of mouse anti-parathyroid hormone (PTH) monoclonal anti-labeled latex fluorescent microspheres

[0044] Take 1mL latex fluorescent microspheres (purchased from Merck Millipore), add 1mg carbodiimide (EDC), 1mg N-hydroxysuccinimide (NHS) and stir at room temperature for 3h, then add 0.1mg of another mouse antiparathyroid hormone Monoclonal antibody, stirred at room temperature for 1 h, then added 10 mg of BSA blocking solution, and continued to stir for 1 h. Centrifuge at 11000r / min for 30min at 2-8°C, and remove the supernatant. Finally, take 1mL of 0.2M phosphate buffer solution (pH=7.4) and add it to the solid precipitate obtained after centrifugation, then add 1μL of Proclin300, shake on a 40Hz ultrasonic oscillator for 1min to mix well, and then store at 4°C stand-by.

[0045] 2) Late...

Embodiment 3

[0068] Embodiment 3 comparison experiment

[0069] The parathyroid hormone kit prepared in Examples 1 and 2 was compared with the Siemens parathyroid hormone detection kit. Detect by collecting 20 patients' fingertip peripheral blood and venous blood (serum, plasma, whole blood), to determine whether the parathyroid hormone test kit prepared in the application of the present invention is applicable to the detection of parathyroid hormone in fingertip peripheral blood Detection, in which the fingertip blood detection was repeated twice. The test results are shown in Table 2 below.

[0070] Table 2 Detection data

[0071]

[0072] In the embodiment of the present invention 1, parathyroid hormone kit detects fingertip peripheral blood and Siemens parathyroid hormone kit detects the detection result of venous blood (serum) and carries out correlation analysis such as Figure 5 As shown, the regression equation is y=1.0257x+1.4929, R 2 =0.9993, n=20, showing that the parathy...

Embodiment 4

[0074] The preparation of embodiment 4 parathyroid hormone kit

[0075] 4.1 Preparation of parathyroid hormone immunochromatographic detection card

[0076] This process is consistent with the process in "1.1 Preparation of Parathyroid Hormone Immunochromatographic Detection Card" in Example 1.

[0077] 4.2 Preparation of release agent

[0078] 463mg of dithiothreitol (DTT), 1.8g of sodium dodecyl sulfate (SDS), 8.77g of ethylenediaminetetraacetic acid (EDTA), 17.65g of sodium citrate, 15.48g of tricitrate Add sodium and 40g of ethanol into the beaker, add water while stirring until completely dissolved, then quantify it to 1000mL with water to obtain a release agent for future use.

[0079] The detection of the parathyroid hormone kit of embodiment 5 embodiment 4

[0080] This process is consistent with the process in "1.3. Detection" of Example 1.

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Abstract

The invention relates to a parathyroid hormone kit for detecting peripheral blood, and a preparation method and an application thereof. The parathyroid hormone kit comprises a parathyroid hormone immunochromatographic detection card and a release agent, wherein the parathyroid hormone immunochromatographic detection card comprises a test strip, the test strip comprises a detection line and a quality control line, the detection line is coated with a mouse-anti-human parathyroid hormone monoclonal antibody, and the quality control line is coated with a goat-anti-rabbit polyclonal antibody. The parathyroid hormone kit provided by the invention is high in sensitivity, high in stability and wide in linear range, and has excellent accuracy and precision.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and relates to a parathyroid hormone kit for detecting peripheral blood, a preparation method and application thereof. Background technique [0002] Parathyroid hormone (Parathyoidhormone, PTH) is synthesized in the parathyroid gland and secreted into the blood. The complete PTH is a single chain polypeptide containing 84 amino acids with a molecular weight of 9500 Daltons. PTH has a half-life of only a few minutes before being broken into individual fragments and cleared from the blood circulation at an extremely rapid rate. The main function of PTH is to increase blood calcium, which is mainly achieved by promoting the release of calcium in the bones and the absorption of calcium in the kidneys of the distal small audience. [0003] The interaction of PTH and calcitonin ensures the stabilization of blood calcium concentration. High blood calcium concentration inhibits PTH secretion, and low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/533G01N33/543
CPCG01N33/533G01N33/54313G01N33/558
Inventor 林斯肖燚胡琛光焦阁
Owner BEIJING ELCOTEQ BIO TECH
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