Three-hole integrated 3D biological printing nozzle and method for preparing multi-layer gel

Abstract

The invention discloses a three-hole integrated 3D biological printing nozzle and a method for preparing a multi-layer gel. The three-hole integrated 3D biological printing nozzle comprises a square nozzle at the bottom and three ink bins, wherein the upper part of the square nozzle respectively communicates with the three ink bins; the three ink bins comprises a superficial layer material bin, amiddle layer material bin and a deep layer material bin and are used for storing three composite ink materials; the vertically arranged square nozzle is sleeved with a vertical sleeve; the superficiallayer material bin and the deep layer material bin are sequentially and annularly connected to the sleeve in a sleeving mode; and the lower parts of the three ink bins penetrate through an ink discharging pipe to communicate with the square nozzle at the bottom. According to the three-hole integrated 3D biological printing nozzle and the method for preparing the multi-layer gel, a bionic tissue structure of a three-layer gel material directly printed at one time is similar to the natural tissue in terms of components and structures, the binding capability is good between layers, the 3D biological printing process for directly constructing the multi-layer gel material by one-step method is achieved, the preparation rate of a bionic material is increased, the waiting time of a patient is shortened, and the success rate and the recovery rate of the operation are increased.

Description

technical field [0001] The invention belongs to the technical field of biomimetic materials, and in particular relates to a preparation process of a multilayer gel biomimetic material, in particular to a three-hole integrated 3D bioprinting nozzle and a method for preparing multilayer gel in one step, specifically a method based on Preparation process of multi-layer gel biomimetic material for "three holes in one" 3D bioprinting nozzle. Background technique [0002] Most of the tissues and organs of the human body have a complex multi-layered structure, each performing a specific physiological function. For example, human articular cartilage is a highly hydrated translucent elastic connective tissue, mainly composed of extracellular matrix, chondrocytes and water, about 2 to 4 mm thick, and can be divided into three layers from the surface to the inside: superficial layer and middle layer and deep. Among them, the shallow surface layer accounts for about 10% to 20% of the ...

AI Technical Summary

Problems solved by technology

However, most of the biomimetic tissues prepared by 3D bioprinting are homogeneous and isotropic at present, and cannot truly mimic the complex multi-layered structures of tissues such as natural cartilage and natural skin.

In addition, even if the construction of multi-layer tissue is achieved through 3D bioprinting technology combined with multiple ink cartridge nozzles, the preparation process is relatively complicated.

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