Medium combination for domestication and production of pleurotus ostreatus and application of medium combination
A technology of mother seed culture medium and original seed culture medium is applied in the field of culture medium combination for the domestication and production of Pleurotus ostreatus, which can solve the problems of low economic benefit, low biotransformation rate, and low survival rate of strains, and achieve good quality , mycelium is white and thick and uniform, and the harvest period is short
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Embodiment 1
[0033] Embodiment 1 Wild Pleurotus ostreatus mother kind substratum cultivation effect
[0034] 1. Materials
[0035] The material used in this test comes from a wild Pleurotus ostreatus strain collected from Sanqiao Town, Daozhen Autonomous County in August 2018. The local people call it "frozen fungus". After morphological observation, related fungal books and pictures, it was initially identified as Pleurotus ostreatus, namely Pleurotus ostreatus. . Through the separation of the fruiting body tissue of wild flat mushroom, the wild flat mushroom bacterial classification is cultivated (see figure 1 and figure 2 ,in figure 1 Field habitat for wild Pleurotus ostreatus; figure 2 It is the fruiting body of wild Pleurotus ostreatus).
[0036] 2. Cultivation effect of wild Pleurotus ostreatus parent species culture medium
[0037] 1) Refer to Table 1 for the formula of the medium for wild Pleurotus ostreatus parent species.
[0038] Table 1 Mother seed culture medium formu...
Embodiment 2
[0049] Embodiment 2 original seed culture medium culture effect
[0050] 1. Materials
[0051] The wild oyster mushroom female species that embodiment 1 formula 2 prepares.
[0052] 2. The cultivation effect of the original seed medium
[0053] 1) See Table 3 for the formulation of the original medium.
[0054] Table 3 original seed medium formula table
[0055]
[0056]
[0057] 2) Production and cultivation of original seed medium
[0058] The original seed medium was processed according to the 6 formulas in Table 3. Each formula was made into 10 bags, each bag was 900g, weighed according to each formula, the culture material was stirred evenly, and the water volume was adjusted to 60%. Fill in propylene plastic bags, sterilize at 121°C and 1.4MPa for 2 hours, and when cooled to room temperature, insert an equal amount of wild oyster mushroom mother species prepared in Formula 2 of Example 1 under aseptic conditions, and cultivate them at 25°C , Determining the my...
Embodiment 3
[0065] Embodiment 3 cultivar medium cultivation effect
[0066] 1. Materials
[0067] The wild oyster mushroom stock that embodiment 2 formula 4 prepares.
[0068] 2. Cultivation effect of cultivated medium
[0069] 1) Refer to Table 5 for cultivar culture medium formula.
[0070] Table 5 cultivar medium formula table
[0071]
[0072] 2) Production and cultivation of cultivar medium
[0073] The cultivar culture medium selects 8 formulas in Table 3, and each formula makes 15 bags, and each bag weighs 900g. Weigh the material according to the formula, stir the compost evenly, adjust the water volume to about 60%, fill it in a 17cm×33cm polypropylene plastic bag, sterilize it at 121℃1.4MPa for 2h, and when it cools down to room temperature, insert it under sterile conditions Equal amount of the wild oyster mushroom stock prepared by formula 4 of Example 2 was cultivated at 25° C., and the mycelium growth status of each formula was measured when bacteria emerged.
[007...
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