Efficient phosphorus-removing saccharomyces and application thereof in domestic sewage treatment

A technology of domestic sewage and yeast, applied in the field of environmental biology, can solve problems such as shortage of phosphorus resources, red tide, damage to water bodies and water ecological environment, etc., to improve treatment efficiency and stability, broaden application research ideas, and enhance practical application value Effect

Inactive Publication Date: 2019-11-12
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Phosphorus is a trace element that all life (animals, plants, microorganisms) depend on for survival, and is an important component of bacterial nucleoprotein, nucleic acid and other substances; Two-thirds of the phosphate rock is used for phosphate fertilizers, and is also used to make phosphoric acid, pesticides, washing powder, etc.; however, phosphorus is a one-way cycle of non-recyclable resources, and its consumption will cause serious losses, even worse than water and oil are more valuable; according to statistics, the annual production of phosphate rock in the world is about 100 million tons, and it will reach the peak of global phosphate rock output in 2035, after which it will face a shortage of phosphorus resources; at the same time, with the continuous development of industrial enterprises, A large amount of phosphorus-containing wastewater is discharged into natural water bodies, destroying the self-purification ability of water bodies, causing serious algal blooms, red tides and other phenomena, and destroying water bodies and water ecological environments; breakthrough
[0003] The biological phosphorus removal method has the advantages of low investment cost, no need to add chemicals, and no secondary pollution. It has become the most widely used wastewater phosphorus removal method; The principle of excessive phosphorus uptake by phosphorus bacteria under aerobic conditions is to achieve the purpose of phosphorus removal in wastewater by discharging excess sludge; however, the reaction conditions of phosphorus accumulating bacteria are relatively complicated, and it is necessary to strictly control the aerobic / anaerobic alternating environment

Method used

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  • Efficient phosphorus-removing saccharomyces and application thereof in domestic sewage treatment
  • Efficient phosphorus-removing saccharomyces and application thereof in domestic sewage treatment
  • Efficient phosphorus-removing saccharomyces and application thereof in domestic sewage treatment

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example 1

[0019] Example 1: Isolation, screening and identification of bacterial strains.

[0020] (1) Material preparation:

[0021] 1. Collect granular filter material from a well-run A / O alternate biofilm system, and make a bacterial suspension, and use the gradient dilution method to inoculate the diluted liquid onto the yeast screening medium for coating and separation of yeast; Use an inoculation loop to pick different types of yeast for streaking and purification; finally, the purified yeast is stored in a -86°C ultra-low temperature refrigerator;

[0022] 2. culture medium

[0023] Yeast screening medium (1L): 20 g peptone, 20 g glucose, 10 g yeast extract, 15~20 g agar powder, 15ug / ml  streptomycin, 60 mg / L chloramphenicol, sterilized at 121 °C 15 minutes;

[0024] YPD solid medium (1L): 20 g peptone, 20 g glucose, 10 g yeast extract, 15-20 g agar powder, sterilized at 121 °C for 15 min;

[0025] YPD liquid medium (1L): 20 g peptone, 20 g glucose, 10 g yeast extract, steri...

example 2

[0048] Example 2 Bacterial strains in the present invention Candida rugosa Culture of BL3.

[0049] (1) Optimizing the culture conditions of bacterial strains in the present invention

[0050] ①Different pH

[0051] Use hydrochloric acid and sodium hydroxide to adjust the pH value to 3, 4, 5, 6, 7, 8, and 9 (3 parallel samples) before the sterilization of the wastewater medium, take 16 mL of bacterial suspension to inoculate, and adjust the OD 600 =0.2±0.02, cultured on a shaker (28 ℃, 220 r / min) for 60 h, and measured OD 600 and PO in the supernatant 4 3- -P concentration, calculate the phosphorus removal rate, see the results figure 2 ;Depend on figure 2 It can be seen that the bacterial strain of the present invention has an OD within the range of 3 to 9 at pH 600 , The phosphorus removal rate increases gradually. When the pH value is 3, the phosphate removal rate of the strain BL3 is only 40.6%; when the pH value is between 5 and 7, the phosphate removal rate of ...

example 3

[0060] Example 3 Bacterial strains in the present invention Candida rugosa Application of BL3 in sewage treatment: Take sewage from the regulating tank of a reclaimed water station in a university in Shandong (the concentration of COD is 400mg / L, the concentration of phosphorus is 11mg / L, and the concentration of ammonia nitrogen is 44 mg / L), and the test of phosphorus removal in wastewater is carried out. The specific steps As follows: Take 350 ml of actual sewage (sterilized) in a 500 ml Erlenmeyer flask, adjust the OD 600 =0.2±0.02, under the conditions of pH 5 and temperature 25 ℃, aerobic culture on a shaking table with a shaking frequency of 220 r / min; , 48 h, and 60 h to take test water samples, take the supernatant after centrifugation, and measure the concentration changes of orthophosphorus, ammonia nitrogen, and COD in the supernatant, and the results are shown in Figure 6 ;Depend on Figure 6 It can be seen that within 12 hours during the aerobic cultivation pr...

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Abstract

The invention relates to efficient phosphorus-removing saccharomyces and application thereof in domestic sewage treatment and belongs to the field of environmental microbiology. The saccharomyces strain is named Candida rugosa BL3 and belongs to Candida rugosa, bacterial colonies are in a light yellow waxy shape and linked by milk white filaments in the middle, and single colonies are milk white round, 2-3 mm, smooth and waxy on surface, not transparent, viscous and easy to prick up, have a strong wine aroma and can form round ascospores, pseudohypha and fungal hypha. The strain has an efficient phosphorus-removing function, requires no strict control on the dissolved oxygen environment, still has no phosphorus release phenomenon after being cultured for 60 h, and can be applied to biological sewage treatment processes. The strain has the optimum pH value range of 5-7 and growth temperature of 20-35 DEG C and adopts a mixed source of glucose and sodium acetate as the best carbon source. Actual domestic sewage treatment finds that the strain has the removal rate of phosphorus in sewage being 84.9%, the removal rate of ammonia nitrogen being 39.5% and the removal rate of COD being 78.3% under an aerobic condition at the pH value of 5 and at the temperature of 25 DEG C.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a high-efficiency phosphorus-removing yeast and its application in domestic sewage treatment. Background technique [0002] Phosphorus is a trace element that all life (animals, plants, microorganisms) depend on for survival, and is an important component of bacterial nucleoprotein, nucleic acid and other substances; Two-thirds of the phosphate rock is used for phosphate fertilizers, and is also used to make phosphoric acid, pesticides, washing powder, etc.; however, phosphorus is a one-way cycle of non-recyclable resources, and its consumption will cause serious losses, even worse than water and oil are more valuable; according to statistics, the annual production of phosphate rock in the world is about 100 million tons, and it will reach the peak of global phosphate rock output in 2035, after which it will face a shortage of phosphorus resources; at th...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/02C02F3/34C12R1/72C02F101/16C02F101/30C02F101/10
CPCC12N1/02C02F3/347C02F2101/105C02F2101/16C02F2101/30C12R2001/72C12N1/165
Inventor 邱立平胡孟飞杨炎明谢康刘贵彩孙绍芳厉鹏远高明昌邱琪程仁振
Owner UNIV OF JINAN
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