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Method for fermentation production of adenine

A technology of adenine and fermentation broth, applied in the field of biopharmaceuticals, can solve the problems of high safety hazard, high production cost, long process route, etc., and achieve the effects of small safety hazard, less equipment investment and wide source of raw materials.

Pending Publication Date: 2019-11-05
CHIFENG BROAD BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two methods have long process routes and use a large amount of organic solvents, which have the problems of high manufacturing costs and large safety hazards.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Inoculate the seeds of adenine into the following medium, glucose 15g / L, corn steep liquor 100g / L, soybean powder 10g / L, yeast powder 5g / L, plant polypeptide powder 10g / L, soybean meal powder 25g / L L, urea 1g / L, glycine 5g / L, alanine 2g / L, ferrous sulfate 1.5g / L, magnesium sulfate 0.5g / L, potassium dihydrogen phosphate 5g / L, diammonium hydrogen phosphate 8g / L, Calcium chloride 2g / L, sodium glutamate 10g / L, methionine 1.5g / L, histidine 0.5g / L, defoamer 0.4ml / L, culture temperature 34°C, air flow 0.3-0.8VVM, fermentation The cycle was 45 hours, and the adenine content was detected to be 12.5g / L.

Embodiment 2

[0028] Embodiment 2: Inoculate the seeds of adenine into the following medium, glucose 30g / L, corn steep liquor 45g / L, soybean powder 5g / L, plant polypeptide powder 10g / L, soybean peptone 15g / L, soybean meal powder 20g / L L, urea 4g / L, glycine 2g / L, alanine 5g / L, ferrous sulfate 0.5g / L, sodium molybdate 0.5g / L, magnesium sulfate 0.5g / L, potassium dihydrogen phosphate 2g / L, Diammonium hydrogen phosphate 2g / L, calcium chloride 5g / L, sodium glutamate 3g / L, methionine 5g / L, histidine 5g / L, defoamer 0.3ml / L, culture temperature 34°C, air flow 0.3-0.8VVM, the fermentation period is 48 hours, and the detected adenine content is 14.3g / L.

Embodiment 3

[0029] Example 3: Inoculate the seeds of adenine into the following medium, glucose 10g / L, corn steep liquor 25g / L, soybean powder 20g / L, yeast powder 15g / L, soybean peptone 15g / L, soybean meal powder 20g / L , urea 3g / L, glycine 3 / L, alanine 3g / L, sodium molybdate 0.5g / L, magnesium sulfate 0.5g / L, potassium dihydrogen phosphate 8g / L, diammonium hydrogen phosphate 6g / L, chlorine Calcium chloride 2g / L, sodium glutamate 3g / L, methionine 2g / L, histidine 2g / L, defoamer 0.25ml / L, culture temperature 34°C, air flow 0.3-0.8VVM, fermentation cycle 46 hours , the detected adenine content was 13.8g / L.

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Abstract

The invention discloses a method for fermentation production of adenine. The method comprises the steps of fermentation cultivation, crude product extraction and fine product extraction in sequence, specifically, fermentation cultivation comprises the steps that adenine seeds are inoculated onto a culture medium, and after fermentation, an adenine fermentation broth containing the adenine is obtained; crude product extraction comprises the steps that the adenine fermentation broth is subjected to the steps of fermentation broth inactivation, solid-liquid separation, filtrate concentration, adsorption elution, eluent concentration, temperature-decreasing crystallization, and an adenine crude product is obtained; and fine product extraction comprises the steps that the adenine crude productis added with purified water to be dissolved and put into activated carbon for decolorization, and crystallization and drying are performed to obtain an adenine fine product. According to the method,raw materials are wide in source, the production cost is low, a process technology is mature, environmental protection is achieved, the product purity is high, and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, in particular to a method for producing adenine by fermentation. Background technique [0002] Adenine, whose chemical name is 6-aminopurine, is an important component of nucleic acid and a key intermediate of many pharmaceutical raw materials. It is currently mainly used in the production of anti-AIDS drug tenofovir disoproxil and anti-hepatitis B drug adefovir dipivoxil, etc. . [0003] At present, the main production methods of adenine include total chemical synthesis, and adenine produced by fermentation combined with chemical synthesis and cleavage as raw materials. These two methods have long process routes and use a large amount of organic solvents, which have the problems of high manufacturing costs and great potential safety hazards. Contents of the invention [0004] The object of the present invention is to provide a method for producing adenine by fermentation. The meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12N1/18C07D473/34C12R1/865
CPCC12P17/182C12N1/18C07D473/34
Inventor 张国银何永进
Owner CHIFENG BROAD BIOLOGICAL TECH CO LTD
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