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Secalonic acid L derived from Penicillium oxalicum and application in inhibiting human cancer cell proliferation

A cancer cell and compound technology, applied in the field of biomedicine, can solve problems such as drugs that have not yet been seen, and achieve the effect of significantly inhibiting the proliferation of cancer cells

Active Publication Date: 2019-11-05
福建省肿瘤医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the indicated ryronic acid compound has anti-human cancer activity, and there is no report on the anti-proliferation activity of this compound on human cancer cells, so there is no drug related to it in the market.

Method used

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  • Secalonic acid L derived from Penicillium oxalicum and application in inhibiting human cancer cell proliferation
  • Secalonic acid L derived from Penicillium oxalicum and application in inhibiting human cancer cell proliferation
  • Secalonic acid L derived from Penicillium oxalicum and application in inhibiting human cancer cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Fermentative production and separation and purification of the compound of embodiment 1

[0020] 1 Fermentation production

[0021] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Penicillium oxalicum ( Penicillium oxalicum ) IBPT-6 (preserved in China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number is: CCTCC NO: M 2013714) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 2 to 3 days.

[0022] Penicillium oxalicum ( Penicillium oxalicum ) Appropriate amount of IBPT-6, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, NaCl 15.0, constant volume] in a 1000mL Erlenmeyer flask, after static culture at 28°C for 30 days, mycelium and fermentation broth were obtaine...

Embodiment 2

[0032] Example 2 Test of anti-tumor activity in vitro

[0033] 1 Experimental samples and experimental methods

[0034] Preparation of test sample solution The test sample is the pure product of the compound isolated and refined in the above-mentioned Example 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0035] The subculture of cell lines and cells adopts tumor cell lines, and the tumor cells are treated with DMEM medium containing 10% FBS at 37°C with 5% CO 2 Subculture in an incubator.

[0036] Cell Proliferation Inhibitory Activity Test Method

[0037] Tetrazolium salt (MTT) method: Take tumor cells in the logarithmic growth phase and adjust the cell density to 2×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2for 4 hours in an incubator. Add 2 microliters of sample solution or blank sol...

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Abstract

The invention relates to secalonic acid L derived from Penicillium oxalicum and application in inhibiting human cancer cell proliferation. The compound has a function of inhibiting human cancer cell proliferation. The compound has a structural formula shown in the specification. Through fermentation culture on Penicillium oxalicum IBPT-6, a fermentation product is obtained, and the compound is separated and purified from the fermentation product. Tests show that the compound has good anti-tumor activity upon multiple human cancer cells. The compound can be applied to preparation of cancer cellproliferation inhibition medicines for research on anti-tumor medicines.

Description

technical field [0001] The invention relates to a ryeconic acid compound Secalonic acid L derived from Penicillium oxalicum and its application in inhibiting the proliferation of human cancer cells, belonging to the field of biomedicine. Background technique [0002] Secalonic acids belong to the secondary metabolites of Ergochrome and are xanthone dimers. Since Stoll et al. isolated Secalonic acid A (Secalonic acid A) from fungi in 1952, this series of compounds Secalonic acid (A-I) has been continuously discovered and studied. Secalonic acid compounds have various physiological activities. Taking Secalonic acid D (Secalonic acid D, SAD) as an example, 5 mg / ml of SAD is added to normal saline, within the range of 5-20 mg, That is, it can treat early bladder cancer, within the range of 50-100 mg, it has curative effect on more serious bladder cancer and has no side effects. It has been found through research that some marine fungi can produce ryryronic acid compounds with ...

Claims

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Application Information

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IPC IPC(8): C07D311/86A61P35/00C12P17/16C12R1/80
CPCC07D311/86A61P35/00C12P17/162
Inventor 刘沁颖郑秋红许扬梅陈立鲁志浩
Owner 福建省肿瘤医院
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