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Heating device and heating method of capillary tube chromatographic column

A capillary chromatographic column and heating device technology, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of high price, inaccurate temperature control, difficult loading and unloading, etc., and achieves the effects of simple production, simple production method and low cost.

Pending Publication Date: 2019-10-25
DONGZHIMEN HOSPITAL OF BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For proteomics research based on the bottom up strategy, the capillary tip column outside the electrospray ion source can be heated and depressurized by the commercial column thermostat that comes with the capillary chromatograph, but commercial heating devices exist Problems such as difficult loading and unloading, inaccurate temperature control, and high price

Method used

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  • Heating device and heating method of capillary tube chromatographic column
  • Heating device and heating method of capillary tube chromatographic column
  • Heating device and heating method of capillary tube chromatographic column

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Embodiment 1, the relationship between the temperature change of the capillary chromatographic column and its column pressure

[0043] Such as figure 2 As shown, a capillary chromatographic column 7 with a size of 30 cm×150 μm and a spray head is used. The inner diameter of the spray head of the capillary chromatographic column 7 used is 150 μm, the particle size of the fully porous reversed-phase chromatography packing is 3 μm, and the pore size is 10 nm. A 30cm×150μm capillary chromatographic column 7 with a spray head is fixed in the liquid phase four-way card slot 5 in the capillary column thermostat 1 through a four-way piece. Such as figure 2 As shown in a), the nozzle end of the capillary chromatographic column 7 is directly above the atmospheric pressure inlet of the mass spectrometer 10 at 2 mm, figure 2In b), the required temperature of the column thermostat 1 can be set in the power supply and temperature controller 11, and the flat end of the capillary ...

Embodiment 2

[0048] Example 2. Application of the liquid mass spectrometry system equipped with the heating device of the present invention in complex sample analysis

[0049] In order to further evaluate the performance of the heating device of the present invention, it is applied to the analysis of normal human urine proteome expression profile. On the LC-MS system equipped with the heating device of the present invention, perform the LC-MS analysis of the urine protease digest under the condition of 15cm and 30cm capillary chromatographic column length, and repeat the experiment once.

[0050] From image 3 As shown, due to the use of the ultra-long 150min chromatographic gradient, the separation efficiency is more obvious for the 30cm capillary column with a longer column length, and more peptide peaks can be separated after 100min, effectively increasing the number of spectral identifications. The data obtained by LC / MS analysis was searched by the maxquant search engine, and the ave...

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Abstract

The invention discloses a heating device and heating method of a capillary tube chromatographic column. The heating device comprises a column temperature box; a heating piece is arranged at the bottomof the column temperature box, and is connected with a temperature sensor; two ends of the heating piece are connected with a DC power supply; the temperature sensor is connected with a temperature controller; and a capillary tube chromatographic column fixing device is arranged above the heating piece in the column temperature box. By adopting a mode of serially connecting the temperature sensor, the temperature controller and a high-heating ceramic heating piece, two ends of the ceramic heating piece are connected with the corresponding interfaces of the DC power supply and the temperaturecontroller; the current flowing through the ceramic heating piece can be changed by arranging the temperature of the temperature controller, namely, the temperature in the capillary tube in the columntemperature box can be regulated; the temperature required by the capillary tube chromatographic column can be obtained according to an actual demand, thereby reducing the moving phase viscosity flowing through the capillary tube chromatographic column, and reducing the negative pressure of a capillary tube chromatograph.

Description

technical field [0001] The invention relates to a heating device and a heating method of a capillary chromatographic column, belonging to the fields of analytical chemistry, biological analysis and proteomics. Background technique [0002] Capillary high performance liquid chromatography-mass spectrometry is widely used in the field of qualitative and quantitative proteome analysis. However, due to the wide variety of proteins in biological samples, the large number of identified proteins, and the wide dynamic distribution range, it is difficult to achieve deep coverage of the proteome, especially for the commonly used capillary chromatography columns with a length of about 15 cm and filled with 3-5 μm particle fillers. For the qualitative and quantitative analysis of low-abundance proteins, the most important thing is that the diagnostic and therapeutic markers related to diseases are usually very low-abundance proteins, so the development of efficient chromatographic separ...

Claims

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Application Information

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IPC IPC(8): G01N30/54
CPCG01N30/54
Inventor 齐宝云
Owner DONGZHIMEN HOSPITAL OF BEIJING UNIV OF CHINESE MEDICINE
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