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Biomass amorphous carbon having oxidase-like and peroxidase-like activity, and preparation method and application thereof

A peroxidase, amorphous carbon technology, applied in the fields of nanomaterials, catalysis and analytical chemistry, can solve the problems of unfavorable popularization and application, complicated operation, expensive detection equipment, etc., and achieves broad application prospects, simple preparation method, and synthetic method. simple effect

Inactive Publication Date: 2019-10-25
QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, methods for detecting ACP activity are generally fluorescence spectrophotometry, electrochemical biosensing, etc., but these methods are relatively complicated to operate, and some require relatively expensive detection equipment, which is not conducive to the popularization and application of actual detection.

Method used

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  • Biomass amorphous carbon having oxidase-like and peroxidase-like activity, and preparation method and application thereof
  • Biomass amorphous carbon having oxidase-like and peroxidase-like activity, and preparation method and application thereof
  • Biomass amorphous carbon having oxidase-like and peroxidase-like activity, and preparation method and application thereof

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preparation example Construction

[0035] In a specific embodiment of the present invention, a kind of preparation method of the biomass amorphous carbon with imitation oxidase and imitation peroxidase activity is provided, and described preparation method comprises:

[0036] S1, placing Enteromorpha in a decolorizing solution for decolorization until Enteromorpha turns white;

[0037] S2. Calcining the Enteromorpha treated in step S1 at a high temperature.

[0038] In yet another specific embodiment of the present invention, in the step S1,

[0039] The decolorizing solution is a mixed solution of sodium hypochlorite, dehydrated alcohol and water;

[0040] The dosage ratio of the sodium hypochlorite, absolute ethanol and water is 10-15g: 5-10mL: 1100-1300mL (preferably 12g: 9mL: 1200mL); by controlling the dosage of the three, it is beneficial to improve the decolorization efficiency of Enteromorpha enteromorpha;

[0041] During the decolorization process, it is preferably placed in a high-temperature enviro...

Embodiment 1

[0052] Preparation of biomass amorphous carbon:

[0053] Put Enteromorpha into a decolorizing solution containing absolute ethanol (9mL), sodium hypochlorite (12g), and water (1200mL), soak in a dry box at 75°C for one hour, then take it out and replace it with a new decolorizing solution to continue soaking, repeat the above Step until the enteromorpha turns white. Afterwards, the decolorized Enteromorpha is washed and put into a vacuum drying oven for drying. Put the dried Enteromorpha into a vacuum tube furnace and calcinate at 900°C for 2h.

Embodiment 2

[0055] Validation of Mimetic Oxidase Activity of Enteromorpha-based Biomass Amorphous Carbon:

[0056] Experimental system a: The catalytic reaction system is composed of acetate buffer (pH 3.3, 100mmol / L), biomass amorphous carbon (30μg / mL) obtained in the above example and organic chromogenic agent TMB (0.5 mmol / L) in React in a water bath at 37°C for 20 minutes, and record its visible absorption spectrum in the range of 400-800nm ​​with a microplate reader.

[0057] Another control experiment b included acetate buffer (pH 3.3, 100mmol / L) and biomass amorphous carbon (30μg / mL) obtained in the above example in the catalytic system, and the rest of the reaction conditions were the same as the above experimental system, 20 minutes Then measure its absorbance. Another control experiment c: the biomass amorphous carbon obtained in the above example is not added to the catalytic reaction system, and its absorbance value is detected after reacting for 20 minutes under the same con...

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Abstract

The invention provides a biomass amorphous carbon having oxidase-like and peroxidase-like activity, and a preparation method and application thereof. With enteromorpha as a raw material, simple high-temperature calcination is performed to obtain biomass amorphous carbon having oxidase-like and peroxidase-like activity simultaneously. According to the biomass amorphous carbon, acid phosphatase is detected qualitatively and quantitatively based on the oxidase-like property. The biomass material with the low cost is available; the preparation method is simple; and the green and environment-friendly effects are good. Therefore, the biomass amorphous carbon has the broad application prospects in analytical chemistry, catalysis, biomedicine and environmental engineering fields.

Description

technical field [0001] The invention belongs to the technical fields of nanomaterials, catalysis and analytical chemistry, and in particular relates to a biomass amorphous carbon with oxidase-like and peroxidase-like activities and a preparation method and application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] In the past few years, extensive studies have been conducted on nanomaterial-based enzyme mimics (nanozymes), which have superior properties compared with natural enzymes, such as low cost, high stability, and easy preparation and It is easy to modify and can replace natural enzymes for a series of catalytic functions. The reported mimetic enzymes gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78C01B32/05
CPCC01B32/05G01N21/78
Inventor 刘爱骅任涵
Owner QINGDAO UNIV
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