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Spherical nano hydroxyapatite/natural polymer biomimetic scaffold and preparation method thereof

A nano-hydroxyapatite, natural polymer technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of uneven distribution, uncontrollable hydroxyapatite particle morphology, size and content, etc. The method is simple, conducive to migration and growth, and the effect of adjustable degradation rate

Active Publication Date: 2019-10-18
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem solved by the present invention is: to provide a preparation method of spherical nano-hydroxyapatite / natural polymer bionic scaffold, to solve the uncontrollable shape, size and content of hydroxyapatite particles and uneven distribution in the prior art question

Method used

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  • Spherical nano hydroxyapatite/natural polymer biomimetic scaffold and preparation method thereof
  • Spherical nano hydroxyapatite/natural polymer biomimetic scaffold and preparation method thereof
  • Spherical nano hydroxyapatite/natural polymer biomimetic scaffold and preparation method thereof

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Embodiment 1

[0031] This example discloses the preparation method of the spherical nano-hydroxyapatite / natural polymer biomimetic scaffold of the present invention. Gelatin is selected as the natural polymer raw material, the soluble calcium salt is calcium chloride, and a glutaraldehyde solution with a mass concentration of 0.25% For cross-linking agent, the implementation steps are as follows:

[0032] (1) After mixing 1 g of gelatin and 18 mL of deionized water, heat and stir in a 60° C. water bath for 30 minutes to obtain a transparent and uniform gelatin solution with a mass fraction of 5%.

[0033] (2) Add 2 mL of glutaraldehyde solution with a mass fraction of 2.5% to the gelatin solution (final concentration of glutaraldehyde is 0.25%), mix well, pour the solution into a plastic petri dish, and place it in a refrigerator at 4°C for 12 hours Make it a gel. After adding 3.6mL calcium chloride solution dropwise to cover the surface of the gel, place it in a refrigerator at 4°C to dif...

Embodiment 2

[0036] Select gelatin as the natural polymer raw material, the soluble calcium salt is calcium nitrate, and 0.1% glutaraldehyde is the cross-linking agent. The implementation steps are as follows:

[0037] (1) After mixing 1 g of gelatin and 18 mL of deionized water, place it in a water bath at 60° C. and heat and stir for 30 minutes to obtain a transparent and uniform gelatin solution with a mass fraction of 5%.

[0038] (2) Add 2 mL of glutaraldehyde solution with a mass fraction of 1% into the gelatin solution (final concentration of glutaraldehyde is 0.1%), mix well, pour the solution into a plastic petri dish, and place it in a refrigerator at 4°C for 12 hours. It becomes a gel. After dripping 6mL of calcium nitrate solution to cover the surface of the gel, place it in a refrigerator at 4°C to diffuse for 12 hours, then add 6mL of phosphoric acid solution to cover the surface of the gel, and place it in a refrigerator at 4°C Continue to diffuse for 12 hours. Among them, t...

Embodiment 3

[0041]Select gelatin as the natural polymer raw material, the soluble calcium salt is calcium chloride, and 0.25% glutaraldehyde is the cross-linking agent. The implementation steps are as follows:

[0042] (1) After mixing 1 g of gelatin and 18 mL of deionized water, place it in a water bath at 60° C. and stir for 30 minutes to obtain a transparent and uniform gelatin solution with a mass fraction of 5%.

[0043] (2) Pour the solution into a plastic petri dish, place it in a refrigerator at 4° C. for 2 hours to solidify the solution into a colloid, take out the colloid in the petri dish and soak it in 20 mL of 0.25% glutaraldehyde solution for 12 hours. Then add 6mL calcium chloride solution dropwise to cover the surface of the gel, place it in a refrigerator at 4°C to diffuse for 6 hours, then add 6mL phosphoric acid solution dropwise to cover the surface of the gel, and continue to spread in a refrigerator at 4°C for 6 hours. Hour. Wherein, the concentration of calcium chl...

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Abstract

The invention discloses a spherical nano hydroxyapatite / natural polymer bionic scaffold and a preparation method thereof. The problems are solved that in the prior art, the particle morphology, size and content of hydroxyapatite are uncontrollable and the distribution is uniform. The preparation method of the spherical nano hydroxyapatite / natural polymer bionic scaffold includes the following steps that a natural polymer is dissolved into deionized water to form a polymer solution; the polymer solution is cross-linked to form polymer gel; a soluble calcium salt solution is dropwise added on the surface of the gel for diffusion; then a soluble phosphorus-containing solution is dropwise added onto the surface of the gel for further diffusion; after diffusion, the solution left on the gel isremoved, the gel is soaked in an alkaline solution, then the gel is washed in sequence with a phosphate buffer solution (PBS) and deionized water, and after freeze drying, the three-dimensional porousbionic scaffold is obtained. The design is scientific, the method is simple and operation is simple and convenient.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a spherical nano-hydroxyapatite / natural polymer bionic scaffold and a preparation method. Background technique [0002] Due to trauma, tumors, population aging and other reasons, there are more and more patients with bone defects, and bone defect diseases have become a major disease that threatens human health. The bone matrix is ​​mainly composed of organic matter and inorganic matter. The organic matter is mainly composed of collagen fibers, and the main component of inorganic matter is hydroxylimestone. Nano-hydroxyapatite crystals are evenly and orderly arranged in the long axis direction of collagen fibers. In order to imitate the composition and structure of natural bone, it is of great significance to combine natural organic polymers (such as collagen, gelatin, etc.) and inorganic hydroxyapatite particles to biomimetically construct bone defect repair materials. [000...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/52A61L27/46A61L27/58
CPCA61L27/46A61L27/52A61L27/56A61L27/58A61L2300/604A61L2430/02
Inventor 李向锋肖玉梅宋滔陈雪宁张兴栋
Owner SICHUAN UNIV
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