Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of he dyed slice

A production method and sectioning technology, which is applied in the field of HE stained sections, can solve the problems of reducing HE production quality, affecting pathological diagnosis, tissue cracks, etc., to increase intercellular adhesion, clear tissue shape, reduce or tissue crack effect

Active Publication Date: 2021-02-02
CENT SOUTH UNIV
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Through clinical and pathological quality control, it is found that the current HE production mainly has the following problems: (1) tissue cracks; (2) cell overlap, extrusion, and poor staining
Such defects reduce the quality of HE production and seriously affect the pathological diagnosis
At present, the above phenomenon exists widely in the pathology departments of hospitals at all levels, and there is no good solution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of he dyed slice
  • A kind of preparation method of he dyed slice
  • A kind of preparation method of he dyed slice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A method for making HE stained slices, comprising the steps of:

[0051] (1) Use a brand-new microtome to cut tissue thin slices (lesioned tissue clamped under the colonoscope) to avoid tissue knife marks, and mount the slides with anti-off slides to obtain white slices;

[0052] (2) Bake the slices in an oven at 65°C for 1 hour to fully adhere the tissue slices to the glass slides and prevent slices from falling off.

[0053] (3) Put the white flakes in turpentine for full dewaxing for 5-10 minutes, and wash them successively with 100%, 95%, and 70% ethanol for 5 minutes each, and then clean the turpentine;

[0054] (4) Put the white tablet in distilled water for 1-2 minutes to hydrate the tissue and restore the pH value of the tissue to neutral;

[0055] (5) Put the white tablet in the EDTA antigen restoration solution diluted 50 times with distilled water, and heat it in a water bath at 60°C for 6 minutes; the above-mentioned EDTA antigen restoration solution is a p...

Embodiment 2

[0070] The preparation method of the HE-stained section in this example is the same as that in Example 1.

[0071] The white flakes obtained in this example come from the breast biopsy tissue after intraoperative quick frozen section examination (this tissue is confirmed by immunohistochemistry to be non-special type invasive carcinoma of the breast).

Embodiment 3

[0078] The preparation method of the HE-stained section in this example is the same as that in Example 1.

[0079] The white flakes obtained in this example are from breast tissue (this tissue was confirmed as invasive micropapillary carcinoma of the breast by immunohistochemistry).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a production method of an HE-stained slice. The production method comprises the steps that a white slice is roasted, dewaxed, hydrated, HE-stained and packaged, wherein the step of treating the white slice, obtained after hydrating treatment, with a metal ion complexant solution is further included between hydrating and HE-staining. According to the production method, whenthe HE-stained slice is produced, treatment with the metal ion complexant solution is added between the hydrating step and the HE-staining step, and a metal ion complexant can effectively reduce or eliminate tissue cracks, so that tissue morphology is clearer; and cell pyknosis and overlapping can be effectively reduced, so that the accuracy and reliability of tissue and cell interpretation are greatly enhanced.

Description

technical field [0001] The invention belongs to the technical field of preparation of pathological tissue slices, in particular to a method for making HE stained slices. Background technique [0002] The premise of precise treatment is precise diagnosis, and the most important thing is precise pathological diagnosis. High-quality HE-stained slides are the basis of clinical pathological diagnosis, and the quality of HE slides will largely affect pathologists' accurate pathological diagnosis. [0003] Through clinical and pathological quality control, it is found that the current HE production mainly has the following problems: (1) tissue cracks; (2) cell overlap, extrusion, and poor staining. Such defects reduce the quality of HE production and seriously affect the pathological diagnosis. At present, the above-mentioned phenomenon exists widely in the pathology departments of hospitals at all levels, and there is no better solution. Contents of the invention [0004] The...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30G01N1/36
CPCG01N1/30G01N1/36G01N2001/302
Inventor 王宽松粟诗童傅春燕谢斌周建华周训检禹灿平马苛文戚嘉琳胡祯敏
Owner CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com