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Anaerobic electrogenesis strain capable of degrading phenol and application thereof

A strain and phenol technology, applied in bacteria, biochemical fuel cells, microbial-based methods, etc., can solve the problem of low Coulombic efficiency of MFC

Active Publication Date: 2019-10-11
HARBIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem that the existing phenol-degrading strains are applied to MFC, and the coulombic efficiency of MFC is low, and provides an anaerobic electricity-producing strain capable of degrading phenol and its application

Method used

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  • Anaerobic electrogenesis strain capable of degrading phenol and application thereof
  • Anaerobic electrogenesis strain capable of degrading phenol and application thereof
  • Anaerobic electrogenesis strain capable of degrading phenol and application thereof

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Experimental program
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Effect test

specific Embodiment approach 1

[0037] Specific embodiment 1: The anaerobic electrogenic strain that can degrade phenol in this embodiment is Enterobacter sp.A4, which is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation address is Wuhan University, Wuhan, and the preservation date is April 2019 On March 11, the deposit number is CCTCC No: M 2019249.

[0038] The Enterobacter sp.A4 of this embodiment is a Gram-negative bacterium, straight rod-shaped, with a cell size of (1.1-1.5 μm)×(2.0-6.0 μm), single or in pairs, moving with perinatal flagella or Non-motile, non-spore-free, non-capsulated, facultatively anaerobic, forming larger red colonies on MacConkey plates.

[0039] Enterobacter sp.A4 of this embodiment can decompose glucose and lactose to produce acid and gas, and acetate can be used as the only carbon source, but citrate cannot be used. h 2 The S test was negative, the indole test was negative, the methyl red test was negative, the V-P test was positive, and ...

specific Embodiment approach 2

[0041] Specific embodiment two: the screening method of Enterobacter sp.A4 of the present embodiment is:

[0042] The microbial fuel cell is selected as a double-chamber MFC, and the volume of the two chambers is 600mL. The anode electrode is made of ferric chloride modified carbon felt (5cm×5cm, effective area 12.56cm 2 ), the cathode electrode uses a carbon brush, and the carbon brush is soaked with 1mol / L HCl and 1mol / L NaCl for 24 hours before use. The cathode chamber and the anode chamber are connected by a salt bridge, and the interface is insulated. The external rheostat box (0-9999Ω) in the circuit adopts a resistance of 1000Ω. The voltage generated during the experiment is directly recorded by the dual-channel voltage acquisition system (PISO813), and the data is measured every 60s.

[0043]Into 20min N before starting the battery 2 , to ensure anaerobic conditions. First, the sewage from the aeration tank and the sewage from the anaerobic tank are mixed at a vol...

specific Embodiment approach 3

[0051] Specific implementation mode 3: Optimization of growth of phenol-degradable anaerobic electrogenic bacteria and phenol degradation conditions

[0052] On the anaerobic operation platform, inoculate the strain on a shaker of separation medium at 30°C and 160r / min for 48h, take 3mL of the culture solution and put it in a centrifuge tube for 3 times of centrifugation (8000r / min), and the bacteria Put into 50mL physiological saline to make bacterial suspension. The bacterial suspension was inoculated into inorganic salt medium and cultured for 48 hours to study the effects of different biological characteristics (nitrogen source, carbon source, pH value, inoculum size, initial phenol concentration, temperature and metal ions) on the growth of the strain and the degradation of phenol . Do 2 repetitions when the strains are inserted into the shake flask, and 3 repetitions when measuring the growth amount and phenol degradation rate, and then take the average value.

[0053]...

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Abstract

The invention relates to an anaerobic electrogenesis strain capable of degrading phenol and application thereof. The problem is solved that by applying an existing phenol degrading strain to MFC, thecoulombic efficiency of the MFC is low. The strain is enteric bacilli Enterobacter sp.A4, is preserved in the China Center For Type Culture Collection and has the preservation date of April 11th, 2019and the preservation number of CCTCC No:M 2019249. The strain is used for establishing a microorganism fuel cell (MFC) for degrading the phenol and is used for improving the coulombic efficiency of the MFC. The strain is used for degrading phenol pollutants in wastewater.

Description

technical field [0001] The invention relates to an anaerobic electricity-producing bacterial strain and application thereof. Background technique [0002] Phenol is a highly toxic aromatic compound in protoplasm, and is often used as a raw material and reaction intermediate in the chemical industry. The chemical structure of phenols is stable and difficult to degrade, and direct discharge will cause serious harm to organisms and the environment. At present, there are physical methods (adsorption method, solvent extraction method), chemical methods (oxidation method, precipitation method and photocatalysis method), biological methods (activated sludge method, enzyme treatment method) and so on to treat phenolic wastewater. Physical and chemical methods are suitable for high-concentration phenol-containing wastewater, and have the disadvantages of high investment and large loss, while biological treatment of phenol-containing wastewater is a low-cost, environmentally friendly...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34H01M8/16C02F101/34C12R1/01
CPCC02F3/34H01M8/16C02F2101/345C12R2001/01C12N1/205Y02E60/50
Inventor 燕红李金达郭改娟
Owner HARBIN UNIV OF SCI & TECH
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