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Preparation method and application of electrochemical catalysis assisted self-enhanced photoelectrochemical immunosensor for detecting procalcitonin

An immune sensor and procalcitonin technology, applied in the field of scientific immune sensors, can solve the problems of difficulty in realizing full automation, narrow linear range, and low sensitivity, and achieve the effects of promoting electron transfer, increasing photoelectric signal intensity, and increasing immobilization capacity

Active Publication Date: 2019-10-01
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many detection methods for sepsis markers, such as enzyme-linked immunoassay, electrochemiluminescence analysis, etc., but enzyme-linked immunoassay has methodological limitations such as low sensitivity and narrow linear range; although electrochemiluminescence analysis The detection linear range is wide and the operation is simple, but it is not easy to realize full automation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 A method for preparing a self-enhanced photoelectrochemical immunosensor for the detection of procalcitonin electrochemically catalyzed

[0036] 1) Add 4 μL, 0.1% (w / v) chitosan solution containing 1% acetic acid dropwise to the porous nanoarray BiVO 4 / CuS electrode surface, continue to drop 4 μL, 2.5% (v / v) glutaraldehyde solution on the modified electrode surface, dry at room temperature, and rinse with ultrapure water;

[0037] 2) Add 8 μL of capture antibody Procalcitonin Ab dropwise 1 Put the solution on the surface of the modified electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0038] 3) Add 3 μL, 1% bovine serum albumin solution dropwise to the surface of the modified electrode, dry it in a refrigerator at 4°C, and rinse with ultrapure water;

[0039] 4) Add 8 μL, 50 fg / mL ~ 100 ng / mL procalcitonin antigen standard solution dropwise on the surface of the modified electrode, dry it in a refrigerator at 4°C, and rinse w...

Embodiment 2

[0041] Example 2 A method for preparing a self-enhanced photoelectrochemical immunosensor for the detection of procalcitonin electrochemically catalyzed

[0042] 1) Add 5 μL, 0.1% (w / v) chitosan solution containing 1% acetic acid dropwise to the porous nanoarray BiVO 4 / CuS electrode surface, continue to drop 5 μL, 2.5% (v / v) glutaraldehyde solution on the modified electrode surface, dry at room temperature, and rinse with ultrapure water;

[0043] 2) Add 10 μL of capture antibody Procalcitonin Ab dropwise 1 Put the solution on the surface of the modified electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0044] 3) Add 4 μL, 1% bovine serum albumin solution dropwise to the surface of the modified electrode, dry it in a refrigerator at 4°C, and rinse with ultrapure water;

[0045] 4) Add 10 μL, 50 fg / mL ~ 100 ng / mL procalcitonin antigen standard solution dropwise on the surface of the modified electrode, dry it in a refrigerator at 4°C, and rinse...

Embodiment 3

[0047] Example 3 A method for preparing a self-enhanced photoelectrochemical immunosensor for the detection of procalcitonin electrochemically catalyzed

[0048] 1) Add 6 μL, 0.1% (w / v) chitosan solution containing 1% acetic acid dropwise to the porous nanoarray BiVO 4 / CuS electrode surface, continue to drop 6 μL, 2.5% (v / v) glutaraldehyde solution on the modified electrode surface, dry at room temperature, and rinse with ultrapure water;

[0049] 2) Add 12 μL of capture antibody Procalcitonin Ab dropwise 1 Put the solution on the surface of the modified electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0050] 3) Add 5 μL, 1% bovine serum albumin solution dropwise on the surface of the modified electrode, dry it in a refrigerator at 4°C, and rinse with ultrapure water;

[0051] 4) Add 12 μL, 50 fg / mL ~ 100 ng / mL procalcitonin antigen standard solution dropwise on the surface of the modified electrode, dry it in a refrigerator at 4°C, and rinse...

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Abstract

The invention relates to a preparation method and application of an electrochemical catalysis assisted self-enhanced photoelectrochemical immunosensor for detecting procalcitonin. According to the preparation method provided by the invention, a porous nano-array BiVO4 / CuS is used as a substrate material, and electrochemical catalysis assisted self-enhanced photocurrent is obtained under visible light irradiation and an anode bias voltage. The two components of the substrate material have good energy band matching, which is beneficial to the separation of electron hole pairs; the photoexcited holes can oxidize water to generate H2O2 under the anode bias voltage, the hole-excited H2O2 can be catalytically reduced by CuS, thereby further effectively suppressing the separation of the electronhole pairs, and improving the intensity of photocurrent. Polystyrene microspheres are used as secondary antibody markers to significantly improve the sensitivity of the sensor, the amounts of combinedsecondary antibody markers are different due to different amounts of procalcitonin to be detected, such that the photocurrent signal response degrees are different. The constructed sensor achieves sensitive detection of the procalcitonin at a detection limit of 17.8 fg / mL.

Description

technical field [0001] The invention relates to the preparation method and application of a self-enhanced photoelectrochemical immunosensor assisted by electrochemical catalysis, specifically using porous nano-array BiVO 4 / CuS as the substrate material and polystyrene microspheres as the secondary antibody marker to prepare a self-enhanced photoelectrochemical immunosensor for sensitive detection of procalcitonin, which belongs to the field of new functional materials and biosensing detection technology. Background technique [0002] Sepsis refers to the acute systemic infection caused by pathogenic bacteria or opportunistic pathogenic bacteria invading the blood circulation, growing and multiplying in the blood, and producing toxins. Sepsis is divided into bacteremia without obvious symptoms of toxemia and sepsis with multiple abscesses. If sepsis is not quickly controlled, it can lead to tissue damage, organ dysfunction and even death. Therefore, early detection and earl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/30G01N27/3278
Inventor 冯金慧魏琴张诺任祥魏东冯锐胡丽华
Owner UNIV OF JINAN
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