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Method for separating and quantitatively analyzing food-borne nanoparticles

A technology for quantitative analysis of nanoparticles, applied in particle and sedimentation analysis, particle size analysis, analysis of materials, etc., can solve the problems of inability to quantitatively analyze and elute food-borne nanoparticles, achieve low detection limit, high sensitivity, Effect of small injection volume

Pending Publication Date: 2019-09-27
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still deficiencies in the above separation methods. The separation methods used are medium separation, and there is a problem that some samples are adsorbed in the column packing and cannot be eluted. Therefore, the above separation methods cannot accurately detect the separated food-borne nanoparticles. quantitative analysis

Method used

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  • Method for separating and quantitatively analyzing food-borne nanoparticles
  • Method for separating and quantitatively analyzing food-borne nanoparticles
  • Method for separating and quantitatively analyzing food-borne nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Separation and collection of river clam soup nanoparticles by capillary electrophoresis

[0042] Take 1 mg of clam soup freeze-dried powder and prepare a solution with a concentration of 1 mg / mL with 1 mL of pure water, collect it by capillary electrophoresis, set the program according to the peak time of nanoparticles, repeat the experiment for 30 rounds, and collect it in a sample bottle. First, the collected samples were taken with a transmission electron microscope, and the results were as follows: figure 1 (Ⅰ). The collected samples were then measured using a Malvern laser particle size analyzer, and the results were as follows Figure 2-8 (II).

[0043] The results showed that the average particle size of the collected samples was 68.4nm, which was consistent with the particle size of nanoparticles separated by ultrafiltration and dialysis. Therefore, it was confirmed that capillary electrophoresis can separate the nanoparticles of clam soup.

Embodiment 2

[0044] Example 2: Quantitative analysis of functional nanoparticles in river clam soup

[0045] Including the following steps:

[0046] (1) Thermal processing, centrifugation, and freeze-drying to obtain functional nano-particle freeze-dried powder in river clam soup

[0047] Wash the clam with distilled water and rest for more than 5 hours, weigh the fresh individual, add distilled water according to the ratio of solid to liquid 1:2kg / L and boil for 1 hour, then cool to room temperature, and finally filter with gauze to obtain the clam soup.

[0048] The clam soup is then centrifuged at a speed of 8000r·min -1 , centrifuged for 15 min to obtain the supernatant.

[0049] Finally, the supernatant was pre-frozen at -80°C for 0.5-3h, and then transferred to a vacuum freeze dryer for 2-3d. Freeze-dry the supernatant to obtain freeze-dried powder.

[0050] (2) Preparation of samples and separation of functional nanoparticles by capillary electrophoresis

[0051] The lyophilized ...

Embodiment 3

[0055] Example 3: Detecting the Effect of Ultraviolet Wavelength

[0056] Take one portion each of 1mg clam original soup freeze-dried powder, ultrafiltration cut-off particle freeze-dried powder and dialysis particle freeze-dried powder, respectively prepare a solution with a concentration of 1mg / mL with 1mL pure water, place it in a quartz cuvette, and at the same time , take 1mL pure water as a blank control. Measure the absorbance of the sample solution at 190-900nm to determine the wavelength corresponding to the maximum absorption peak of the original soup and its separated nanomicelle. The results are as follows figure 2 show.

[0057] The results show that when the absorption wavelength is 214nm, the absorbance value of the sample solution is the largest, except that the original solution of river clam soup has two larger absorbance values, and the prepared nanoparticles have no interference from other impurities. Therefore, the determination of nanomicelle The sele...

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Abstract

The invention discloses a method for separating and quantitatively analyzing food-borne nanoparticles. The method comprises the following steps of: (1) performing Corbicula fluminea hot processing to obtain Corbicula fluminea soup, centrifuging the Corbicula fluminea soup to obtain a supernatant, and performing freeze-drying treatment of the supernatant to obtain freeze-dried powder; (2) separating the functional nano particles in the freeze-dried powder solution by using capillary electrophoresis; and (3) calculating the concentration or number per volume of functional nanoparticles obtained in the step (2) by a linear regression equation. The method has the advantages of rapid separation, the good separation effect, the high sensitivity, the good reproducibility, the low detection limit, the small sample injection amount, capability of performing quantitative analysis on the functional nanoparticles and the like.

Description

technical field [0001] The invention belongs to the field of food and nanometer material analysis, in particular to a method for separation and quantitative analysis of foodborne nanometer particles. Background technique [0002] Capillary Electrophoresis (Capillary Electrophoresis, CE), also known as High Performance Capillary Electrophoresis (HPCE), the separation site is a capillary (inner diameter 10-200nm), the driving force is a high-voltage electric field, and the charged particles are different according to their mobility and distribution coefficient Electrophoresis technology for rapid separation. Based on the following advantages, capillary electrophoresis has been widely used in separation and analysis: (1) high separation efficiency, column efficiency can reach 10 7 / m or more; (2) fast speed, the detection time is usually more than ten seconds to ten minutes; (3) wide range of analysis, from small molecular substances to macromolecular polymers, used in life sc...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N15/02G01N27/447G01N1/40
CPCG01N1/40G01N15/0205G01N21/33G01N27/447G01N2001/4038
Inventor 杨多佳余兆硕柯李晶高观祯汪惠勤周建武饶平凡邹坚桥彭彰文
Owner ZHEJIANG GONGSHANG UNIVERSITY
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