Fermentation medium, application thereof and method for preparing lycopene through fermentation of Blakeslea trispora

Abstract

The invention belongs to the field of production of lycopene and discloses a fermentation medium, the application thereof and a method for preparing lycopene through fermentation of Blakeslea trispora. The fermentation medium comprises the following ingredients: a starch component, a bean flour component, fish meal, vegetable oil, copper sulfate, magnesium sulfate, monopotassium phosphate, vitamin E and an emulsifying agent, wherein the starch component comprises starch and / or starch hydrolysate; the bean flour component comprises bean flour and / or bean flour hydrolysate; and at least one of the starch component and the bean flour component is the hydrolysate of the starch or bean flour. With the adoption of the fermentation medium provided by the invention, the concentration of dissolved oxygen can be improved, and the dissolved oxygen can be reasonably and efficiently utilized. Therefore, the yield of lycopene is improved.

Description

technical field [0001] The invention belongs to the field of lycopene production, and in particular relates to a fermentation medium and its application and a method for preparing lycopene by fermentation of B. trispora. Background technique [0002] Carotenoids are important pigments that widely exist in nature. They are compounds with similar structures derived from isoprene as the molecular center through terminal cyclization, oxygen addition, or bond rotation and isomerization. Lycopene is one of the more important carotenoids. Lycopene is a fat-soluble antioxidant that can be synthesized by many plants and microorganisms, but not by animals and humans. It is also called lycopene because it was first found in tomatoes, and it is also found in various tissues and organs of the human body. Multi-distribution, the unsaturated structure contained in its molecule makes it have strong antioxidant activity and the ability to scavenge free radicals, which can effectively preven...

AI Technical Summary

Problems solved by technology

[0006] The fermentation medium of B. trispora mostly uses starch and soybean powder as carbon and nitrogen sources, but this fermentation medium has a great influence on the transfer of oxygen, and in the early stage of fermentation, the rapid growth of the bacteria makes it The demand for dissolved oxygen and nutrients is relatively high, and dissolved oxygen becomes a key factor that limits the growth of bacteria and product synthesis, and even causes metabolic abnormalities, affecting the output of target products

[0007] Therefore, in the fermentation process of producing lycopene with B. trispora as the strain, researchers often screen excellent strains through mutagenesis or change the stirring form of fermentation and the way of dissolved oxygen supply, and add oxygen carrier or Adding surfactants that can increase the permeability of bacterial cells to increase the oxygen concentration and oxygen utilization in the fermentation broth has a certain effect on the improvement of the fermentation process, but there are also shortcomings of one kind or another, or The final yield is low, or the added substances have certain damage to the bacteria itself, or the cost is high due to the complexity of the equipment, or it is only for one aspect without comprehensive consideration of the fermentation process

Disclosed in CN1331343A is a kind of technology that the form of agitating blade is changed, adopts the upper middle layer to be the wide-leaf propelling type, the lower floor is the combined agitating blade of turbine type, although can improve the mass transfer performance of fermented liquid like this, but change The stirring form can only be suitable for the production characteristics of the strain, which is not conducive to the comprehensive utilization of the fermenter

CN101555490A adds active carbon to fermentation broth to increase dissolved oxygen content, but this method has problems such as increasing equipment loss and maintenance costs, and complex extraction process

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