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Application of AMPH (amphiphilic protein) cytokine to preparation of hepatic failure treatment drug

A liver failure and cytokine technology, applied in the fields of clinical medicine, molecular medicine and biomedicine, can solve the problems of death of patients and shortage of donor livers

Inactive Publication Date: 2019-09-27
杭州笙源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But due to a severe shortage of donor livers, a large number of patients died while waiting for a liver transplant

Method used

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  • Application of AMPH (amphiphilic protein) cytokine to preparation of hepatic failure treatment drug
  • Application of AMPH (amphiphilic protein) cytokine to preparation of hepatic failure treatment drug
  • Application of AMPH (amphiphilic protein) cytokine to preparation of hepatic failure treatment drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Injection containing AMPH cytokines treats a large animal (young pig) model of liver failure

[0043] Animal model: 30 male Chinese young pigs (8-10 kg) were randomly divided into two groups, 15 in each group. Each young pig was injected with D-gal 1.5g / kg in the jugular vein to create a liver failure model.

[0044] Test group: Multiple intravenous injections of AMPH injection at fixed time points after D-gal injection, dose: 10ml / kg, twice a day.

[0045] Control group: inject the same amount of normal saline without AMPH.

[0046] Neither the control group nor the experimental group received other drug treatment.

[0047] __ figure 1 It is a schematic diagram of the survival time curves of the young pigs of the experimental group and the control group, showing the survival rate of the young pigs of the experimental group and the control group. The results showed that the 3-day survival rate of young pigs in the treatment group containing AMPH cytoki...

Embodiment 2

[0048] Embodiment 2: The lyophilized powder injection containing AMPH cytokine treats the small animal (rat) model of liver failure

[0049] Animal model: 100 male rats (200-250 g) were randomly divided into two groups, 50 in each group. Each rat was intraperitoneally injected with D-gal 1.5g / kg to make a liver failure model. The AMPH lyophilized powder and water for injection are prepared into a suspension.

[0050] Experimental group: 4 ml of AMPH lyophilized powder suspension was injected intraperitoneally at a fixed time point after D-gal injection, twice a day.

[0051] Control group: inject the same amount of normal saline without AMPH.

[0052] Both the control group and the experimental group received no other drug treatment.

[0053] figure 2 Schematic diagram of the survival time curves of rats in the experimental group and the control group, figure 2The survival rates of rats in the experimental group and the control group are shown: the results show that the...

Embodiment 3

[0054] Embodiment 3: The suspension containing AMPH cytokine treats the rabbit model of hepatic failure

[0055] Animal model: 40 adult male rabbits (2000-2500 g) were randomly divided into two groups, 20 in each group. Each rabbit was intramuscularly injected with D-gal 1.5g / kg to make a liver failure model.

[0056] Test group: Intramuscular injection of AMPH suspension 20ml at a fixed time point after D-gal injection, twice a day.

[0057] Control group: inject the same amount of normal saline without AMPH.

[0058] Both the control group and the experimental group received no other drug treatment.

[0059] image 3 It is a schematic diagram of the survival time curves of rabbits in the experimental group and the control group, image 3 The survival rate of the rabbits in the experimental group and the control group is shown: the results show that the survival rate of the rabbits in the AMPH treatment group is 88% at 1 day and 80% at 7 days, while the survival rate of t...

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Abstract

The invention discloses an application of an AMPH (amphiphilic protein) cytokine to preparation of a hepatic failure treatment drug. The drug comprises a pharmaceutically acceptable excipient, antioxidant and carrier of the AMPH cytokine. On the basis of multi-center and large prospective cohort populations and samples, cross validation is executed from different angles with multiple techniques including transcriptome sequencing, multi-omics association analysis, qRT-PCR (quantitative reverse transcription-polymerase chain reaction), in-vivo validation in animal experiments and the like, and it is indicated that the AMPH cytokine can be applied to effective preparation of the hepatic failure treatment drug and the AMPH cytokine can cooperate with different solvents and stabilizers for producing drugs of different forms and dosages. Selectable administration modes in the using process comprise intravenous injection, intramuscular injection, hypodermic injection and oral administration. AMPH JNK (Jun N-terminal kinase) is one of MAPK family members, and a JNK pathway can inhibit hepatocyte apoptosis by regulating and controlling expression of AMPH.

Description

technical field [0001] The invention belongs to the fields of clinical medicine, molecular medicine and biomedicine, and is a new technology for treating liver failure with AMPH cytokine series drugs, specifically, the application of AMPH cytokines in the preparation of medicaments for treating liver failure. Background technique [0002] Liver failure is a kind of disease caused by extensive necrosis of the liver caused by various reasons. Except for orthotopic liver transplantation, there is currently no specific treatment. However, due to the severe shortage of donor livers, a large number of patients died while waiting for liver transplantation. Clarifying the pathogenesis of liver failure and early treatment targeting the mechanism can effectively block the progression of the disease and reduce the mortality rate, which is of great significance to the treatment of liver failure. [0003] Amphiphilic protein (AMPH) is a protein rich in nerve endings. It is a 128kD prot...

Claims

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Application Information

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IPC IPC(8): A61K38/19A61P1/16
CPCA61K38/19A61P1/16
Inventor 李君陈新李江辛娇娇
Owner 杭州笙源生物科技有限公司
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